A Systematic Review and Meta-Analysis Study to Investigate the Prevalence of Helicobacter pylori and the Sensitivity of its Diagnostic Methods in Iran

Kiani

et al. 2015

J Infect Dev Ctries

Self Cite

The varieties of infections caused by Helicobacter pylori may be due to differences in bacterial genotypes and virulence factors as well as environmental and host-related factors. This study aimed to investigate the prevalence of cagA and vacA genes among H. pylori-infected patients in Iran and analyze their relevance to the disease status between two clinical groups via a meta-analysis method. Different databases including PubMed, ISI, Scopus, SID, Magiran, Science Direct, and Medlib were investigated, and 23 relevant articles from the period between 2001 and 2012 were finally analyzed. The relevant data obtained from these papers were analyzed by a randomeffects model. Data were analyzed using R software and STATA. The prevalence of cagA and vacA genes among H. pylori-infected patients was 70% (95% CI, 64-75) and 41% (95% CI, 24.3-57.7), respectively. The prevalence of duodenal ulcers, peptic ulcers, and gastritis among cagA+ individuals was 53% (95% CI, 20-86), 65% (95% CI, 34-97), and 71% (95% CI, 59-84), respectively. Odds ratio (OR) between cagA-positive compared with cagA-negative patients showed a 1.89 (95% CI, 1.38-2.57) risk of ulcers. In conclusion, the frequency of cagA gene among H. pylori strains is elevated in Iran and it seems to be more frequently associated with gastritis. Therefore, any information about cagA and vacA prevalence among different H. pylori-infected clinical groups in the country can help public health authorities to plan preventive policies to reduce the prevalence of diseases associated with H. pylori infection.

Section: Discussionmentioning

confidence: 99%

Prevalence of cagA and vacA among Helicobacter pylori-infected patients in Iran: a systematic review and meta-analysis

Kiani

et al. 2015

J Infect Dev Ctries

Self Cite

“…On the other hand, the prevalence among younger generations was low ( 4 ). A review and meta-analytic study showed that the mean prevalence of H. pylori was 50.7% during 1994- 2011 with the lowest and highest prevalence in Tehran (19.2%) and Mazandaran (74.27%) provinces, respectively ( 5 ). A survey in Tehran showed that 87.6% of patients with dyspepsia were infected with H. pylori ( 6 ).…”

Section: Introductionmentioning

confidence: 99%

Sero-prevalence of Helicobacter pylori Infection in Neyshabur, Iran, During 2010-2015

et al. 2017

Backgrounds & Objective: The Helicobacter pylori prevalence has continuously decreased during recent years in Iran. The current study aimed at determining H . pylori prevalence in Neyshabur city, Northeast Iran, during 2010-2015. Methods: The current epidemiologic survey was conducted in Neyshabur from 2010 to 2015 to determine the prevalence of H. pylori infection. A total of 11596 participants (3681 male with the mean age of 31.7±6.2 years and 7915 female with mean age of 68.3±4.7 years) were included. The enzyme-linked immunosorbent assay kits for the detection of H. pylori and Stat Fax 3200® Microplate Reader (USA) with a sensitivity of 95% and specificity of 98% were used. Titers above 12 units were considered positive for IgG, IgA, and IgM (negative <8, equivocal 8 to 12, and positive >12 U). The Chi-square t test and F test were used to analyze data. Results and Conclusion: The overall IgA, IgG, and IgM seropositive samples among the study participants were 852 (7.2%), 9000 (72.8%), and 1256 (5.2%), respectively. The IgA seropositivity was significantly high among the age group above 51 years, compared with the other age groups. Moreover, the IgG and IgM seropositivity were significantly high among the age groups 41 to 50 and 31 to 40 years respectively, compared with the other age groups. There was no significant difference between male and female cases regarding IgA and IgG seropositive samples, but IgM level was significantly higher among females, compared with that of the male cases. Furthermore, there was no significant alteration in IgA, IgG, and IgM seropositivity during 2010-2014 in Neyshabur. The prevalence of H. pylori in Neyshabur was high in the healthy population. Furthermore, the H. pylori prevalence did not change from 2010 to 2014 in the studied city. Effective approaches to improve health, educational, and socioeconomic status should be implemented to minimize and control H. pylori infection.

Jundishapur J Microbiol

“…Helicobacter pylori is a spiral-shaped, Gram-negative, microaerophilic and fastidious bacterium ( 1 , 2 ), and infects almost 50% of the world’s population ( 3 , 4 ). It is the main cause of chronic gastritis, gastric and duodenal ulcers, mucosa-associated lymphoid tissue lymphoma and gastric adenocarcinoma ( 2 , 5 ). In the international agency for research on cancer (IARC), H. pylori has introduced as a first class (definitive) carcinogen ( 6 ).…”

Section: Introductionmentioning

confidence: 99%

Development and Diagnostic Evaluation of Loop-Mediated Isothermal Amplification Using a New Gene Target for Rapid Detection of Helicobacter pylori

Bakhtiari¹,

Alvandi²,

Pajavand³

et al. 2016

BackgroundHelicobacter pylori cause chronic gastritis and subsequent diseases like gastric and duodenal ulcers and gastric adenocarcinoma. Current methods for detecting H. pylori have several disadvantages and it is of utmost importance to develop a simple, quick, accurate, and cost-effective diagnostic test.ObjectivesThe aim of this study was to set up and evaluate a diagnostic value of loop- mediated isothermal amplification (LAMP) for detecting H. pylori.Patients and MethodsThe analytical sensitivity values (limit of detection) of LAMP and polymerase chain reaction (PCR) were determined using serial dilutions of H. pylori DNA. Analytical specificity of the methods using new designed primers targeted ureC gene was also determined.ResultsThe detection limits of the LAMP and PCR assay were similar and were 10 fg of pure DNA of H. pylori, which is equal to 6 copy numbers of H. pylori genome. Analytical specificity of the tests was 100% because the tests were positive only with H. pylori DNA.ConclusionsThe analytical sensitivity of LAMP and PCR methods, using the designed primers, was 8 times more than any other reported methods. The designed methods are specific and sensitive for detection of H. pylori in different clinical and environmental samples.