A systematic review of randomized controlled trials on sterilization methods of extracted human teeth

Abstract

Aim of this Study:The aim was to evaluate the efficiency of different sterilization methods on extracted human teeth (EHT) by a systematic review of in vitro randomized controlled trials.Methodology:An extensive electronic database literature search concerning the sterilization of EHT was conducted. The search terms used were “human teeth, sterilization, disinfection, randomized controlled trials, and infection control.” Randomized controlled trials which aim at comparing the efficiency of different methods of sterilization of EHT were all included in this systematic review.Results:Out of 1618 articles obtained, eight articles were selected for this systematic review. The sterilization methods reviewed were autoclaving, 10% formalin, 5.25% sodium hypochlorite, 3% hydrogen peroxide, 2% glutaraldehyde, 0.1% thymol, and boiling to 100°C. Data were extracted from the selected individual studies and their findings were summarized.Conclusion:Autoclaving and 10% formalin can be considered as 100% efficient and reliable methods. While the use of 5.25% sodium hypochlorite, 3% hydrogen peroxide, 2% glutaraldehyde, 0.1% thymol, and boiling to 100°C was inefficient and unreliable methods of sterilization of EHT.

Diş Hekimliği Klinik Öncesi Eğitiminin İlk İki Yılında Öğrenci Yaralanmalarının Araştırılması ve Yaralanma Sonrası Davranışlarının İncelenmesi

“…They also estimate that there is a 2% risk associated with Hepatitis C Virus (HCV) and 6-30 % (if the affected person is not vaccinated) from patients infected with Hepatitis B Virus (HBV). 25 Considering that many students have low HBV and Hepatitis A Virus (HAV) antibody values and that there is a risk of both direct transmission and cross-infection in their preclinical education 16,17 , the rates of not reporting injuries are worrying. In addition, almost all of the students, who had not yet been injured in preclinical courses, stated that they would report their injuries after possible accidents which they may experience in the future.…”

“…1,14,15 Students who receive preclinical education should take the same precaution in preclinical injuries, even if they are not in direct contact with infectious substances such as the patient's blood and saliva. 16 The Occupational Safety and Health Administration's (OSHA) Bloodborne Pathogens Standard recognizes human teeth as a potential source of blood-borne pathogens. 17 In a study evaluating sterilization methods applied to human extracted teeth, it was stated that commonly used sodium hypochlorite sodium hypochlorite solutions (5.25 %) were insufficient and unreliable.…”

“…This result indicates that more care should be taken in preventing infectious diseases due to injuries in preclinical trainings. 16 Another important issue is that students report their injuries during their preclinical education. When dentistry students do not report their occupational injuries that may be exposed to blood-borne pathogens, they may not be able to take necessary precautions and treatments on time as they should.…”

Effect of Er,Cr:YSGG on Remineralization Using CPP - ACPF (MI - Paste Plus) after Enamel Erosion Caused by Carbonated Soft Drink in Primary Teeth: In-Vitro Study

“…A review by Western and Dicksit concluded that autoclaving, and formalin could be efficiently used for sterilisation of extracted teeth. Their use was discouraged, as autoclaving disrupts organic matrix of the tooth and formalin possess a major health hazard as a potential carcinogen [29]. The authors of the current study aimed for intermediate disinfection time, as performed by a previously [19].…”

“…Thymol was also used to disinfect primary teeth in previous researches studying laser assisted remineralisation [22]. However, the authors of this study preferred the use of sodium hypochlorite due to its superior efficacy [29]. Teeth were air dried for only 3 sec before DD measurements as prolonged drying increases the fluorescence readings [21].…”