A systematically optimized awake mouse fMRI paradigm

Xu, Wenjing; Pei, Mengchao; Zhang, Kaiwei; Tong, Chuanjun; Bo, Binshi; Feng, Jianfeng; Zhang, Xiao‐Yong; Liang, Zhifeng

doi:10.1101/2022.11.16.516376

Cited by 5 publications

(15 citation statements)

References 73 publications

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“…We combined Cre-dependent virus (AAV2/9-hSyn-DIO-ChrimsomR-mCheery-WPRE-pA) and VGLUT2-, ChAT-, PV-and SOM-Cre mice to express of ChrimsomR in glutamatergic, cholinergic, PV and SOM GABAergic neurons in BF (Figure 1A and S1). To accommodate the limited space in cryogenic coil, flexible PMMA optical fibers were used in combination with our previous awake mouse fMRI [26][27][28] to establish the awake mouse opto-fMRI setup (Figure 1A-D). The biband EPI pulse sequence 28 was employed to achieve faster acquisition (TR = 500 ms) while maintaining whole brain coverage (in-plane resolution = 150 μm 2 with 38 axial slices).…”

Section: Optogenetic Stimulations Of Bf Neurons Evoke Distinct Cell-t...mentioning

confidence: 99%

“…To accommodate the limited space in cryogenic coil, flexible PMMA optical fibers were used in combination with our previous awake mouse fMRI [26][27][28] to establish the awake mouse opto-fMRI setup (Figure 1A-D). The biband EPI pulse sequence 28 was employed to achieve faster acquisition (TR = 500 ms) while maintaining whole brain coverage (in-plane resolution = 150 μm 2 with 38 axial slices).…”

Section: Optogenetic Stimulations Of Bf Neurons Evoke Distinct Cell-t...mentioning

confidence: 99%

“…Using our optimized awake mouse opto-fMRI setup and standardized data processing protocol (Figure1A and Figure S2) [26][27][28][29] , we observed robust and cell-type specific large-scale BOLD fMRI activations (Figure 1E-F, Figure S3-6). Considering the high spatial similarities between 0.5-s duration and 2-s duration for four BF cell-types (Figure S3), we chose to pool EPI runs with 0.5-s and 2-s durations together, and only focused on the cell-type specific activations (Figure 1E…”

Section: Optogenetic Stimulations Of Bf Neurons Evoke Distinct Cell-t...mentioning

confidence: 99%

“…After 1-week recovery from the fiber and head holder implantation, mice were habituated for awake fMRI for seven days (Figure 1A). Mice were head-fixed on an animal bed with the recorded acoustic MRI scanning noise, following a previously described habituation paradigm 28 . The detailed schedule was listed in below table.…”

Section: Habituation For Awake Mouse Optogenetic Fmri (Opto-fmri)mentioning

confidence: 99%

“…A T2-weighted structural image was acquired for co-registration with following parameters: TR = 3400 ms, TE = 33 ms, field of view = 16 × 16 mm 2 , matrix size = 256 × 256, slice thickness = 0.5 mm, 30 axial slices, RARE factor = 8, and number of averages = 2. After field map based local shimming within the mouse brain, Biband-EPI was acquired using the multiband EPI sequence 28…”

Section: Optogenetic Fmri Acquisitionmentioning

confidence: 99%

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Cell-type specific optogenetic fMRI on basal forebrain reveals functional network basis of behavioral preference

Zou

Tong

Peng

et al. 2023

Preprint

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The basal forebrain (BF) is an anatomically and neurochemically complex structure that plays key roles in regulating various brain functions. Although BF is best known for cholinergic neurons that influence functional network dynamics and behaviors, non-cholinergic neurons are the dominant neuronal population in BF, and are found to contribute as well. However, it remains unclear how cholinergic and non-cholinergic BF neurons modulate large-scale functional networks and their relevance in intrinsic and extrinsic behaviors. With our optimized awake mouse optogenetic fMRI approach, we revealed optogenetic stimulations of four BF neuron types evoked distinct cell-type specific whole-brain BOLD activations, which could attribute to BF-originated low dimensional structural networks. Optogenetic activation of inhibitory (PV and SOM) or excitatory (VGLUT2 and ChAT) neurons in BF drove internally or externally oriented behavioral preference, respectively. Furthermore, we uncovered the functional network basis of the above BF-modulated behavioral preference, through a decoding model linking the BF-modulated BOLD activations, low dimensional structural networks, and behavioral preference. To summarize, we decoded a functional network basis of differential behavioral preference with cell-type specific optogenetic fMRI on BF, and provided an avenue for investigating mouse behaviors from a whole-brain view.

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Section: Optogenetic Stimulations Of Bf Neurons Evoke Distinct Cell-t...mentioning

confidence: 99%

Section: Optogenetic Stimulations Of Bf Neurons Evoke Distinct Cell-t...mentioning

confidence: 99%

Section: Optogenetic Stimulations Of Bf Neurons Evoke Distinct Cell-t...mentioning

confidence: 99%

Section: Habituation For Awake Mouse Optogenetic Fmri (Opto-fmri)mentioning

confidence: 99%

Section: Optogenetic Fmri Acquisitionmentioning

confidence: 99%

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Cell-type specific optogenetic fMRI on basal forebrain reveals functional network basis of behavioral preference

Zou

Tong

Peng

et al. 2023

Preprint

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Sleep fMRI with simultaneous electrophysiology at 9.4 T in male mice

Yang

Qiu

et al. 2023

Nat Commun

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Sleep is ubiquitous and essential, but its mechanisms remain unclear. Studies in animals and humans have provided insights of sleep at vastly different spatiotemporal scales. However, challenges remain to integrate local and global information of sleep. Therefore, we developed sleep fMRI based on simultaneous electrophysiology at 9.4 T in male mice. Optimized un-anesthetized mouse fMRI setup allowed manifestation of NREM and REM sleep, and a large sleep fMRI dataset was collected and openly accessible. State dependent global patterns were revealed, and state transitions were found to be global, asymmetrical and sequential, which can be predicted up to 17.8 s using LSTM models. Importantly, sleep fMRI with hippocampal recording revealed potentiated sharp-wave ripple triggered global patterns during NREM than awake state, potentially attributable to co-occurrence of spindle events. To conclude, we established mouse sleep fMRI with simultaneous electrophysiology, and demonstrated its capability by revealing global dynamics of state transitions and neural events.

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High-resolution awake mouse fMRI at 14 Tesla

Hike,

Liu,

Xie

et al. 2023

Preprint

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High-resolution awake mouse fMRI remains challenging despite extensive efforts to address motion-induced artifacts and stress. This study introduces an implantable radiofrequency (RF) surface coil design that minimizes image distortion caused by the air/tissue interface of mouse brains while simultaneously serving as a headpost for fixation during scanning. Using a 14T scanner, high-resolution fMRI enabled brain-wide functional mapping of visual and vibrissa stimulation at 100x100x200µm resolution with a 2s per frame sampling rate. Besides activated ascending visual and vibrissa pathways, robust BOLD responses were detected in the anterior cingulate cortex upon visual stimulation and spread through the ventral retrosplenial area (VRA) with vibrissa air-puff stimulation, demonstrating higher-order sensory processing in association cortices of awake mice. In particular, the rapid hemodynamic responses in VRA upon vibrissa stimulation showed a strong correlation with the hippocampus, thalamus, and prefrontal cortical areas. Cross-correlation analysis with designated VRA responses revealed early positive BOLD signals at the contralateral barrel cortex (BC) occurring 2 seconds prior to the air-puff in awake mice with repetitive stimulation, which was not detectable with the randomized stimulation paradigm. This early BC activation indicated learned anticipation through the vibrissa system and association cortices in awake mice under continuous training of repetitive air-puff stimulation. This work establishes a high-resolution awake mouse fMRI platform, enabling brain-wide functional mapping of sensory signal processing in higher association cortical areas.Significance StatementThis awake mouse fMRI platform was developed by implementing an advanced implantable radiofrequency (RF) coil scheme, which simultaneously served as a headpost to secure the mouse head during scanning. The ultra-high spatial resolution (100x100x200µm) BOLD fMRI enabled the brain-wide mapping of activated visual and vibrissa systems during sensory stimulation in awake mice, including association cortices, e.g. anterior cingulate cortex and retrosplenial cortex, for high order sensory processing. Also, the activation of barrel cortex at 2 s prior to the air-puff indicated a learned anticipation of awake mice under continuous training of the repetitive vibrissa stimulation.

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A systematically optimized awake mouse fMRI paradigm

Cited by 5 publications

References 73 publications

Cell-type specific optogenetic fMRI on basal forebrain reveals functional network basis of behavioral preference

Cell-type specific optogenetic fMRI on basal forebrain reveals functional network basis of behavioral preference

Sleep fMRI with simultaneous electrophysiology at 9.4 T in male mice

High-resolution awake mouse fMRI at 14 Tesla

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