A tabulated review of capillary electrophoresis of carbohydrates

Abstract

This review summarizes publications on capillary electrophoresis (CE) of carbohydrates, covering almost all hitherto published papers on this topic. It is designed to be a convenient tool for the literature search by providing a comprehensive table. Since CE analysis of carbohydrates is generally complicated due to the structural diversity of carbohydrate species, an attempt is made in this table to supply detailed information on the analyzed form (underivatized or derivatized, type of derivative) and analytical conditions (capillary size, state of the inner wall, composition of the electrophoretic solution, applied voltage, detection method, etc.), for each combination of carbohydrate species to be analyzed. In addition, a brief overview is presented to help in the literature search.

Recent Developments in Liquid Chromatography and Capillary Electrophoresis for the Analysis of Glycoprotein Glycans

“…MEKC using SDS/phosphate/borate, diaminobutane/borate, diaminobutane/phosphate, polyamine/borate, and quaternary alkylammonium/borate was previously reported for the glycoform separation of glycoproteins. The addition of Ni 2+ or Mg 2+ to the electrophoresis buffer has also been applied to the separation of proteins (Review 168 ). Recently, a method of derivatization through the thiol groups of glycoproteins, using either 5-(iodoacetamide)fluorescein (5-IAF) or BODIPY iodoacetamide, has been used to produce a model protein of AGP that was separated into more than 10 peaks by CE using 10 mM tricine/10 mM sodium acetate/10 mM NaCl/7M urea/4.5 mM putresceine, pH 4.5 with acetic acid.…”

Specific Extraction of Sialic-acid-containing Glycans and Glycopeptides Using Serotonin-bonded Silica

“…[1][2][3][4] Therefore, the monitoring of glycan structures accompanying biological processes must be important for understanding the function of glycans in cell biology and pathology. [5][6][7][8][9] Investigation of the role of carbohydrates in the biological function of glycoproteins is often hindered because the low-level analysis of these molecules is considerably more difficult than that for other biopolymers-particularly nucleic acids and proteins. Various analytical methods met difficulty in accurate and precise determination of glycans because the samples often fall below the calibration range.…”

Overview on advances in capillary electrophoresis‐mass spectrometry of carbohydrates: A tabulated review

“…Moreover, CE gives the possibility to analyze, together with carbohydrates, a wide range of nonsaccharidic compounds that might be present in the sample [19,20]. Such features make CE very suitable for the analysis of saccharides, as summarized in various reviews (see for instance [11,[21][22][23]). In the effort to increase as much as possible the amount of information obtainable from a small amount of sample, the coupling of CE with MS for carbohydrate analysis is a really promising approach, due to the informative potential originated by a selective separative method and a highly informative detection technique [24].…”