A TaqMan Real-Time PCR Assay for Quantifying Type III Hepatopancreatic Parvovirus Infections in Wild Broodstocks and Hatchery-Reared Postlarvae of Fenneropenaeus chinensis in Korea

Abstract

A highly sensitive and specific TaqMan real-time PCR was used to quantify hepatopancreatic parvovirus (HPV) type III infections in wild broodstocks and hatcheryreared postlarvae (PL) of Fenneropenaeus chinensis. Totals of 159 and 162 wild brooders from three locations were captured, and 140 and 180 PL were obtained from seven and six commercial hatcheries in 2007 and 2008, respectively. Among the three wild broodstock groups from 2007, only 1 group showed HPV infection and 3.2% of 159 brooders were positive for HPV infection. In 2008, HPV infections were observed from all three wild broodstock groups with 1.93×10 4 copies/mg tissue of pleopods. Of 162 brooders, 26.6% were positive for HPV infection. No PL from the two hatcheries collected in 2007 showed HPV infection, and PL from the rest of the five hatcheries had up to 1.74×10 6 copies/ng of DNA, and PL from three hatcheries showed HPV infections with over 1,000 copies/ng of DNA. The PL from all seven hatcheries collected in 2008 showed up to 2.10×10 5 HPV copies/ng of DNA. PL from two hatcheries showed less than 100 copies/ng of DNA, but PL from the rest of the hatcheries showed HPV infections with over 1,000 copies/ng of DNA. These results show that HPV type III is widely distributed in Korea in addition to previously reported HPV type I, and they can be effectively detected by type-specific realtime PCR.

Effect of biofloc on the survival and growth of the postlarvae of three penaeids (Litopenaeus vannamei, Fenneropenaeus chinensis, and Marsupenaeus japonicus) and their biofloc feeding efficiencies, as related to the morphological structure of the third maxilliped

“…In South Korea, two native penaeids, Fenneropenaeus chinensis (Osbeck, 1765) and Marsupenaeus japonicus (Bate, 1888), and an introduced species, Litopenaeus vannamei (Boone, 1931), are currently cultured, and the majority of the farmed production is derived from L. vannamei (Jang et al, 2009). Shrimp are one of the most economically important aquaculture species in Korea and worldwide.…”

“…Shrimp are one of the most economically important aquaculture species in Korea and worldwide. However, the farmed production of shrimp has been largely influenced by pathogens including white spot syndrome virus (WSSV) in Korea (Meng et al, 2010;Jang et al, 2011). For example, 32.9% of 468 shrimp farms in South Korea collapsed as a consequence of the WSSV outbreak in 2006, although production has recovered since then (Jang et al, 2009).…”

“…However, the farmed production of shrimp has been largely influenced by pathogens including white spot syndrome virus (WSSV) in Korea (Meng et al, 2010;Jang et al, 2011). For example, 32.9% of 468 shrimp farms in South Korea collapsed as a consequence of the WSSV outbreak in 2006, although production has recovered since then (Jang et al, 2009). Recently, culture methods using biofloc technology (BFT) have been proposed as a tool to reduce water exchange and to minimize the introduction of pathogens (Browdy et al, 2001;Burford et al, 2003;Samocha et al, 2007).…”

Development of Two Quantitative Real-Time PCR Diagnostic Kits for HPV Isolates from Korea

“…Despite its worldwide distribution, including the Asia-Pacific region, HPV has not been regarded as a major shrimp pathogen, probably because of heavy infection and severe economic losses caused by WSSV. However, recent real-time PCR surveys of shrimp tissue and seawater sampled from culture ponds of F. chinensis and L. vannamei showed high incidence rates of HPV in Korea [9][10][11].…”

“…Three genotypes of HPV have been reported to date, and in situ hybridization and nucleotide sequence analysis showed that the HPV strain from Korea belongs to genotype I [27,37]. However, recent PCR analysis of shrimp showing HPV symptoms failed to produce any amplification product using a type I-specific 1120F/1120R primer set [11,25]. HPV isolates from different hosts or geographical regions are known to show genetic differences [37].…”