A TEM-2 beta-lactamase encoded on an active Tn1-like transposon in the genome of a clinical isolate of Stenotrophomonas maltophilia

Avison, Matthew B.; Heldreich, Charlotte J. von; Higgins, Catherine S.; Bennett, Peter M.; Walsh, Timothy R.

doi:10.1093/jac/46.6.879

Cited by 71 publications

(33 citation statements)

References 17 publications

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“…S. maltophilia strain K279a (American Type Culture Collection [ATCC] strain BAA-2423) served as our wild-type strain (Table 1). K279a is a multidrug-resistant strain that was isolated from the blood of a cancer patient (18). Mutants of K279a that were used in this study are listed in Table 1.…”

Section: Methodsmentioning

confidence: 99%

Stenotrophomonas maltophilia Encodes a Type II Protein Secretion System That Promotes Detrimental Effects on Lung Epithelial Cells

2013

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The Gram-negative bacterium Stenotrophomonas maltophilia is increasingly identified as a multidrug-resistant pathogen, being associated with pneumonia, among other infections. Despite this increasing clinical problem, the genetic and molecular basis of S. maltophilia virulence is quite minimally defined. We now report that strain K279a, the first clinical isolate of S. maltophilia to be sequenced, encodes a functional type II protein secretion (T2S) system. Indeed, mutants of K279a that contain a mutation in the xps locus exhibit a loss of at least seven secreted proteins and three proteolytic activities. Unlike culture supernatants from the parental K279a, supernatants from multiple xps mutants also failed to induce the rounding, detachment, and death of A549 cells, a human lung epithelial cell line. Supernatants of the xps mutants were also unable to trigger a massive rearrangement in the host cell's actin cytoskeleton that was associated with K279a secretion. In all assays, a complemented xpsF mutant behaved as the wild type did, demonstrating that Xps T2S is required for optimal protein secretion and the detrimental effects on host cells. The activities that were defined as being Xps dependent in K279a were evident among other respiratory isolates of S. maltophilia. Utilizing a similar type of genetic analysis, we found that a second T2S system (Gsp) encoded by the K279a genome is cryptic under all of the conditions tested. Overall, this study represents the first examination of T2S in S. maltophilia, and the data obtained indicate that Xps T2S likely plays an important role in S. maltophilia pathogenesis.

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Section: Methodsmentioning

confidence: 99%

Stenotrophomonas maltophilia Encodes a Type II Protein Secretion System That Promotes Detrimental Effects on Lung Epithelial Cells

2013

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“…Strains were routinely cultured at 37°C in LB broth or on LB agar (Oxoid Ltd., Basingstoke, United Kingdom). M9 minimal salts medium was used in some cases and was prepared using a base of 6 g/liter Na 2 HPO 4 , 3 g/liter KH 2 PO 4 , 1 g/liter NH 4 Cl, and 0.5 g/liter NaCl in water. Liquid cultures were grown with vigorous aeration (150 rpm) in conical flasks with foam bungs, where the culture occupied one-fifth of the total flask volume.…”

Section: Methodsmentioning

confidence: 99%

YieJ (CbrC) Mediates CreBC-Dependent Colicin E2 Tolerance in Escherichia coli

et al. 2010

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Colicin E2-tolerant (known as Cet2) Escherichia coli K-12 mutants overproduce an inner membrane protein, CreD, which is believed to cause the Cet2 phenotype. Here, we show that overproduction of CreD in a Cet2 strain results from hyperactivation of the CreBC two-component regulator, but CreD overproduction is not responsible for the Cet2 phenotype. Through microarray analysis and gene knockout and overexpression studies, we show that overexpression of another CreBC-regulated gene, yieJ (also known as cbrC), causes the Cet2 phenotype.

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“…A TEM-2 ␤-lactamase on a Tn1-like transposon in the genome of S. maltophilia clinical isolate J675Ia was reported (18). The transposon was able to be mobilized onto the broad-host-range conjugative plasmid R388 and moved into E. coli UB1832, demonstrating the ability of S. maltophilia to harbor and exchange DNA with other bacteria.…”

Section: ])mentioning

confidence: 98%

“…maltophilia exhibits resistance to a broad array of antibiotics, including TMP-SMX, ␤-lactam antibiotics, macrolides, cephalosporins, fluoroquinolones, aminoglycosides, carbapenems, chloramphenicol, tetracyclines, and polymyxins. The low membrane permeability that contributes to resistance to ␤-lactams including cefepime, ticarcillin-clavulanate, ceftazidime, and piperacillintazobactam (5,68,229) and the presence of chromosomally encoded multidrug resistance efflux pumps (6,11,54,129,196,198,269,383), ␤-lactamases (9,17,18,227,295,296,352,353), and antibiotic-modifying enzymes (174,190,195) all contribute to the intrinsic antibiotic resistance of S. maltophilia (298). The intrinsic resistance of S. maltophilia was suggested to have been acquired in natural nonhuman environments and is not due solely to the use of antibiotics in medical/clinical settings (218,298).…”

Section: Emergence Of Antibiotic Resistancementioning

confidence: 99%

“…The drug resistance mechanisms are acquired by the horizontal transfer of antibiotic resistance through plasmids, transposons, integrons, integron-like elements, insertion element common region (ISCR) elements, and biofilms (17,18,27,146,198,328). The molecular mechanisms of antibiotic resistance in S. maltophilia are described in more detail below (see "Antibiotic Resistance").…”

Section: Emergence Of Antibiotic Resistancementioning

confidence: 99%

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Stenotrophomonas maltophilia: an Emerging Global Opportunistic Pathogen

2012

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SUMMARY Stenotrophomonas maltophilia is an emerging multidrug-resistant global opportunistic pathogen. The increasing incidence of nosocomial and community-acquired S. maltophilia infections is of particular concern for immunocompromised individuals, as this bacterial pathogen is associated with a significant fatality/case ratio. S. maltophilia is an environmental bacterium found in aqueous habitats, including plant rhizospheres, animals, foods, and water sources. Infections of S. maltophilia can occur in a range of organs and tissues; the organism is commonly found in respiratory tract infections. This review summarizes the current literature and presents S. maltophilia as an organism with various molecular mechanisms used for colonization and infection. S. maltophilia can be recovered from polymicrobial infections, most notably from the respiratory tract of cystic fibrosis patients, as a cocolonizer with Pseudomonas aeruginosa . Recent evidence of cell-cell communication between these pathogens has implications for the development of novel pharmacological therapies. Animal models of S. maltophilia infection have provided useful information about the type of host immune response induced by this opportunistic pathogen. Current and emerging treatments for patients infected with S. maltophilia are discussed.

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A TEM-2 beta-lactamase encoded on an active Tn1-like transposon in the genome of a clinical isolate of Stenotrophomonas maltophilia

Cited by 71 publications

References 17 publications

Stenotrophomonas maltophilia Encodes a Type II Protein Secretion System That Promotes Detrimental Effects on Lung Epithelial Cells

Stenotrophomonas maltophilia Encodes a Type II Protein Secretion System That Promotes Detrimental Effects on Lung Epithelial Cells

YieJ (CbrC) Mediates CreBC-Dependent Colicin E2 Tolerance in Escherichia coli

Stenotrophomonas maltophilia: an Emerging Global Opportunistic Pathogen

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