A tethered bilayer sensor containing alamethicin channels and its detection of amiloride based inhibitors

Yin, Ping; Burns, Christopher J.; Osman, Péter; Cornell, Bruce

doi:10.1016/s0956-5663(02)00160-4

Cited by 60 publications

(39 citation statements)

References 27 publications

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“…Such cushions have consisted of hydrogels, polymeric tethers, polymer films and polyelectrolyte multilayers (PEMs) [18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37]. PEMs offer the following advantages [29][30][31][32][33][34]36]: (1) they are robust and easy to fabricate, (2) they can be deposited on virtually any surface, (3) they can provide a reservoir for electron transfer mediators and cofactors for sensor applications, and (4) their porosity and flexibility may allow the protein to exist in its natural conformation while bound to the BLM.…”

Section: Introductionmentioning

confidence: 99%

Arrays of lipid bilayers and liposomes on patterned polyelectrolyte templates

Kohli¹,

Vaidya²,

Ofoli³

et al. 2006

Journal of Colloid and Interface Science

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Section: Introductionmentioning

confidence: 99%

Arrays of lipid bilayers and liposomes on patterned polyelectrolyte templates

Kohli¹,

Vaidya²,

Ofoli³

et al. 2006

Journal of Colloid and Interface Science

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“…To date, only a few membrane proteins have been successfully incorporated into supported lipid bilayers for electrochemical measurements, and they are mainly limited to small ion channels that insert easily in native conformation into lipid bilayers also when there is only a small aqueous reservoir between the lipid bilayer and the support. Examples of such peptides are gramicidin, melittin, alamethicin, and valinomycin [271,272,273,274,275]. Larger proteins forming ion channels, like Outer membrane protein F (OmpF) from E. Coli, and other peptides have also been investigated [274,276,277,278,279].…”

Section: Supported Lipid Bilayer Sensor Architecturesmentioning

confidence: 99%

Electrochemical Biosensors - Sensor Principles and Architectures

Grieshaber

MacKenzie

Vörös

et al. 2008

Sensors

855

636

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Quantification of biological or biochemical processes are of utmost importance for medical, biological and biotechnological applications. However, converting the biological information to an easily processed electronic signal is challenging due to the complexity of connecting an electronic device directly to a biological environment. Electrochemical biosensors provide an attractive means to analyze the content of a biological sample due to the direct conversion of a biological event to an electronic signal. Over the past decades several sensing concepts and related devices have been developed. In this review, the most common traditional techniques, such as cyclic voltammetry, chronoamperometry, chronopotentiometry, impedance spectroscopy, and various field-effect transistor based methods are presented along with selected promising novel approaches, such as nanowire or magnetic nanoparticle-based biosensing. Additional measurement techniques, which have been shown useful in combination with electrochemical detection, are also summarized, such as the electrochemical versions of surface plasmon resonance, optical waveguide lightmode spectroscopy, ellipsometry, quartz crystal microbalance, and scanning probe microscopy. The signal transduction and the general performance of electrochemical sensors are often determined by the surface architectures that connect the sensing element to the biological sample at the nanometer scale. The most common surface modification techniques, the various electrochemical transduction mechanisms, and the choice of the recognition receptor molecules all influence the ultimate sensitivity of the sensor. New nanotechnology-based approaches, such as the use of engineered ion-channels in lipid bilayers, the encapsulation of enzymes into vesicles, polymersomes, or polyelectrolyte capsules provide additional possibilities for signal amplification. In particular, this review highlights the importance of the precise control over the delicate interplay between surface nano-architectures, surface functionalization and the chosen sensor transducer principle, as well as the usefulness of complementary characterization tools to interpret and to optimize the sensor response.

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“…In water solutions the thermodynamics drive these molecules to assemble into structures such as self-assembled monolayer membranes (SAMs) and self-assembled bilayer membranes (BLMs). SAMs offer promising possibilities in the control over the orientation of the molecule immobilised [48] and BLMs have recently been used in various experimental biosensors as models of actual living cell membranes [49,50]. Molecular self-assembly occurs in nature and produces nanosystems far more advanced than man-made systems.…”

Section: Self-assembled Biomembranesmentioning

confidence: 99%

Biosensors for environmental monitoring of endocrine disruptors: a review article

Rodríguez-Mozaz

Marco

Alda

et al. 2004

Analytical and Bioanalytical Chemistry

136

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This article provides an overview of the applications of biosensors in analysis and monitoring of endocrine-disrupting compounds (EDCs) in the environment. Special attention is devoted to the various types of physical-chemical signal transduction elements, biological mechanisms employed as sensing elements and techniques used for immobilisation of the bioreceptor molecules on the transducer surface. Two different classes of biosensors for EDCs are considered: biosensors that measure endocrine-disrupting effects, and biosensors that respond to the presence of a specific substance (or group of substances) based on the specific recognition of a biomolecule. Several examples of them are presented to illustrate the power of the biosensor technology for environmental applications. Future trends in the development of new, more advanced devices are also outlined.

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A tethered bilayer sensor containing alamethicin channels and its detection of amiloride based inhibitors

Cited by 60 publications

References 27 publications

Arrays of lipid bilayers and liposomes on patterned polyelectrolyte templates

Arrays of lipid bilayers and liposomes on patterned polyelectrolyte templates

Electrochemical Biosensors - Sensor Principles and Architectures

Biosensors for environmental monitoring of endocrine disruptors: a review article

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