A tetrahedral DNA nanostructure-decorated electrochemical platform for simple and ultrasensitive EGFR genotyping of plasma ctDNA

Wang, Xuyao; Wu, Jianping; Mao, Weilin; He, Xiaoqing; Ruan, Li-Ming; Zhu, Junlan; Shu, Peng; Zhang, Zhenqi; Jiang, Bitao; Zhang, Xingguo

doi:10.1039/d0an00591f

Cited by 14 publications

(6 citation statements)

References 42 publications

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“…As reported in the literature, the DNA origami triangles, stored at 4 °C in low-salt buffers, were stable for 2 months . The tetrahedral DNA nanostructure-decorated electrochemical platform, stored at 4 °C, remained stable for 7 days . Positive attempts were made to improve the stability of the DNA nanostructures in some studies.…”

Section: Results and Discussionmentioning

confidence: 64%

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A Specific Nucleic Acid Microfluidic Capture Device Based on Stable DNA Nanostructure

Huang

Chen

et al. 2021

ACS Appl. Mater. Interfaces

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Benefiting from superior programmability and good biocompatibility, DNA nanomaterials have received considerable attention with promising prospects in biological detection applications. However, their poor stability and operability severely impede further development of the applications of DNA nanomaterials. Here, a thermally stable DNA nanomesh structure is integrated into a microfluidic chip. The specificity of the nucleic acid microfluidic capture device could reach the single-base mismatch level while capturing the ssDNA sample. The microfluidic chip provides a closed environment for the DNA nanomesh, giving the device excellent storage stability. After 6 months of storage at room temperature, the device still has a specific capture function on ssDNA samples with low concentration. The specific nucleic acid microfluidic capture device can be applied to the enrichment of ctDNA in the future and contribute to the early diagnosis of cancer.

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Section: Results and Discussionmentioning

confidence: 64%

“…16 The tetrahedral DNA nanostructure-decorated electrochemical platform, stored at 4 °C, remained stable for 7 days. 31 Positive attempts were made to improve the stability of the DNA nanostructures in some studies. The robust 6-helix DNA tile tube was built using a topological structure.…”

Section: Resultsmentioning

confidence: 99%

A Specific Nucleic Acid Microfluidic Capture Device Based on Stable DNA Nanostructure

Huang

Chen

et al. 2021

ACS Appl. Mater. Interfaces

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“…EC assays often involve PCR amplification of the DNA fragment where the mutation site is present, followed by a specific capture of the amplicons by a mutation-specific probe. This was shown, for instance, for point mutations in TP53 [75], KRAS [74], EGFR [69,70,105], or BRCA1 genes [68]. For example, Xu et al reported EC biosensor that analyzes in-frame deletions in exon 19 of an EGFR gene [69].…”

Section: Pcr-based Ec Biosensorsmentioning

confidence: 99%

Electrochemical biosensors for analysis of DNA point mutations in cancer research

et al. 2022

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Cancer is a genetic disease induced by mutations in DNA, in particular point mutations in important driver genes that lead to protein malfunctioning and ultimately to tumorigenesis. Screening for the most common DNA point mutations, especially in such genes as TP53, BRCA1 and BRCA2, EGFR, KRAS, or BRAF, is crucial to determine predisposition risk for cancer or to predict response to therapy. In this review, we briefly depict how these genes are involved in cancer, followed by a description of the most common techniques routinely applied for their analysis, including high-throughput next-generation sequencing technology and less expensive low-throughput options, such as real-time PCR, restriction fragment length polymorphism, or high resolution melting analysis. We then introduce benefits of electrochemical biosensors as interesting alternatives to the standard methods in terms of cost, speed, and simplicity. We describe most common strategies involved in electrochemical biosensing of point mutations, relying mostly on PCR or isothermal amplification techniques, and critically discuss major challenges and obstacles that, until now, prevented their more widespread application in clinical settings.

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“…Our previous work has also proposed an on-site electrochemical platform for the sensitive simultaneous genotyping of the two major EGFR mutations (19del and L858R) through plasma ctDNA based on TDN-decorated SPGE. 95 As shown in Fig. 5B, Linear-After-The-Exponential (LATE)-PCR combined with amplification refractory mutation system (ARMS) was adopted to produce abundant biotin-labeled single-stranded DNA with high amplification efficiency and specificity.…”

Section: Spe Modified With Functional Dna Nanostructuresmentioning

confidence: 99%

Screen-printed electrode-based biosensors modified with functional nucleic acid probes and their applications in this pandemic age: a review

Zhang

Liu

et al. 2022

Anal. Methods

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A tetrahedral DNA nanostructure-decorated electrochemical platform for simple and ultrasensitive EGFR genotyping of plasma ctDNA

Abstract: In this study, we propose an on-site electrochemical platform for sensitive simultaneous genotyping of the two major EGFR mutations (19del and L858R) through plasma ctDNA based on tetrahedral DNA nanostructure decorated screen-printed electrodes.

Cited by 14 publications

References 42 publications

A Specific Nucleic Acid Microfluidic Capture Device Based on Stable DNA Nanostructure

A Specific Nucleic Acid Microfluidic Capture Device Based on Stable DNA Nanostructure

Electrochemical biosensors for analysis of DNA point mutations in cancer research

Screen-printed electrode-based biosensors modified with functional nucleic acid probes and their applications in this pandemic age: a review

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