A tetramerization domain in prokaryotic and eukaryotic transcription regulators homologous to p53

Bernardo, Nerea; Cuppari, Anna; Meijer, Wilfried J. J.; Boer, Roeland

doi:10.1107/s2059798323001298

Cited by 3 publications

(1 citation statement)

References 41 publications

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“…In parallel, an anti-repressor, termed Rap, is held inactive through binding to a short quorum-sensing signaling peptide, Phr, encoded by the plasmid. The transition to an "ON" state is facilitated by a decrease in Phr concentration, and the consequent liberation of Rap, which counteracts Rco, thus promoting conjugation [23][24][25][26] .…”

Section: Introductionmentioning

confidence: 99%

A conjugative plasmid exploits flagella rotation as a cue to facilitate its transfer

Bhattacharya,

Bejerano-Sagie,

Ravins

et al. 2024

Preprint

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Conjugation-mediated DNA delivery is the primary mode for antibiotic resistance spread; yet, molecular mechanisms regulating the process remain largely unexplored. While conjugative plasmids typically rely on solid surfaces to facilitate donor-to-recipient proximity, the pLS20 conjugative plasmid, prevalent among Gram-positiveBacillusspp., uniquely requires fluid environments to motivate its transfer. Here we unveiled that pLS20, carried byB. subtilis, induces adhesin-promoted multicellular clustering, which can accommodate various species, offering a stable platform for DNA delivery in liquid milieu. We further discovered that induction of pLS20 promoters, governing crucial conjugative genes, hinges on the presence of donor cell flagella, the major bacterial motility organelle. Moreover, pLS20 regulatory circuit is strategically integrated into a mechanosensing signal transduction pathway responsive to flagella rotation, harnessing propelled flagella to activate conjugation genes exclusively during the host motile phase. This flagella-conjugation coupling strategy, provides the plasmid with the benefit of disseminating into remote destinations, infiltrating new niches.

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Section: Introductionmentioning

confidence: 99%

A conjugative plasmid exploits flagella rotation as a cue to facilitate its transfer

Bhattacharya,

Bejerano-Sagie,

Ravins

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

Flagellar rotation facilitates the transfer of a bacterial conjugative plasmid

Bhattacharya,

Bejerano-Sagie,

Ravins

et al. 2024

EMBO J

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Conjugation-mediated DNA delivery is the primary mode for antibiotic resistance spread in bacteria; yet, molecular mechanisms regulating the conjugation process remain largely unexplored. While conjugative plasmids typically require bacterial attachment to solid surfaces for facilitation of donor-to-recipient proximity, the pLS20 conjugative plasmid, prevalent among Gram-positive Bacillus spp., uniquely requires fluid environments to enhance its transfer. Here, we show that pLS20, carried by Bacillus subtilis, induces multicellular clustering, which can accommodate various species, hence offering a stable platform for DNA delivery in a liquid milieu. We further discovered that induction of pLS20 promoters, governing crucial conjugative genes, is dependent on the presence of donor cell flagella, the major bacterial motility organelle. Moreover, the pLS20 regulatory circuit is controlled by a mechanosensing signal transduction pathway responsive to flagella rotation, thus activating conjugation gene expression exclusively during the host motile phase. This flagella-conjugation coupling strategy may allow the dissemination of the plasmid to remote destinations, allowing infiltration into new niches.

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Evidences of an inhibitory effect and other functions of human P53 inEscherichia coli

Ben Abid,

Yacoubi,

Djemal

et al. 2024

Preprint

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The human tumor suppressor P53 has so far been shown to inhibit growth in the yeast model as well as in other eukaryotic contexts. Despite a considerable number of sudies involving p53 in bacteria, the question of effects on cell growth and viability has never been explored. In this work, we report similar negative effect on cell viability of the protein expressed inEscherichia colistrain BL21(DE3). This inhibition still needs to be characterized in lights of the distinction between yeast and other organisms with different P53-caused deaths and pathways. Primary tests leaned towards an active p53 in this bacterial context, both under normal and stresseful conditions. Special effects were noticed either with phage infection or antibiotic treatment, using a GST-p53 fusion form. These results open the way for further investigations involving P53 in prokaryote systems.

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A tetramerization domain in prokaryotic and eukaryotic transcription regulators homologous to p53

Cited by 3 publications

References 41 publications

A conjugative plasmid exploits flagella rotation as a cue to facilitate its transfer

A conjugative plasmid exploits flagella rotation as a cue to facilitate its transfer

Flagellar rotation facilitates the transfer of a bacterial conjugative plasmid

Evidences of an inhibitory effect and other functions of human P53 inEscherichia coli

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