A textile-reinforced composite vascular graft that modulates macrophage polarization and enhances endothelial cell migration, adhesion and proliferation <i>in vitro</i>

Zhang, Fan; Tao, Hui; Gluck, Jessica M.; Wang, Lu; Daneshmand, Mani A.; King, Martin W.

doi:10.1039/d2sm01190e

Cited by 5 publications

(2 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although the integrin binding sites within the GelMA hydrogel were observed to support M2 polarization, we found that GelMA hydrogel did not induce an M2 phenotype in 2D cell culture . A more potent induction method of M2 macrophages is by following the interleukin 4 (IL-4) signaling pathway .…”

Section: Introductionmentioning

confidence: 96%

“…6 Although the integrin binding sites within the GelMA hydrogel were observed to support M2 polarization, 5 we found that GelMA hydrogel did not induce an M2 phenotype in 2D cell culture . 7 A more potent induction method of M2 macrophages is by following the interleukin 4 (IL-4) signaling pathway. 8 Delivering IL-4 from a tissue engineering scaffold is a potential way to promote an M2 macrophage response from the scaffold material and to facilitate tissue regeneration.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Heparin Affinity-Based IL-4 Delivery to Modulate Macrophage Phenotype and Endothelial Cell Activity In Vitro

Zhang

Gluck

Brown

et al. 2023

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

Macrophages play a pivotal role in wound healing and tissue regeneration, as they are rapidly recruited to the site of injury or implanted foreign material. Depending on their interaction with the material, macrophages can develop different phenotypes, with the M1 pro-inflammatory and M2 pro-regenerative phenotypes being highly involved in tissue regeneration. M2 macrophages mitigate inflammation and promote tissue regeneration and extracellular matrix remodeling. In this study, we engineered a gelatin-heparin-methacrylate (GelMA-HepMA) hydrogel that gradually releases interleukin-4 (IL-4), a cytokine that modulates macrophages to adopt the M2 phenotype. Methacrylation of heparin improved the retention of both heparin and IL-4 within the hydrogel. The GelMA-HepMA hydrogel and IL-4 synergistically downregulated M1 gene expression and upregulated M2 gene expression in macrophages within 48 h of in vitro cell culture. However, the M2-like macrophage phenotype induced by the GelMA-HepMA-IL-4 hydrogel did not necessarily further improve endothelial cell proliferation and migration in vitro.

show abstract

Section: Introductionmentioning

confidence: 96%