A theoretical study of multicomponent radial flow chromatography

Gu, Tingyue; Tsai, Guo-Jane; Tsao, George T.

doi:10.1016/0009-2509(91)85055-3

Cited by 30 publications

(10 citation statements)

References 22 publications

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“…Consequently the solute will be concentrated [4]. It was proved to be a promising alternative to conventional axial flow chromatography (AFC) [5]. Its relatively large flow area and short flow path allow fast separations and low pressure drops [6,7] and it has been used for a wide range of separations [8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Study on the purification of polysaccharides from Noscoc flagelliforme with radial flow chromatography

Dai

Wang

Jia

et al. 2009

Biotechnol Bioproc E

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^Äëíê~Åí= The isolation and purification of polysaccharide from kçëÅçÅ=Ñä~ÖÉääáÑçêãÉ by radial flow chromatography were studied. The column (7.7 cm of bed length and 229.6 cm 3 of bed volume) was packed with DEAE-01 anion ion-exchange resin and gradient eluted with NaCl solutions. The content of the polysaccharide was determined with the phenol-sulfuric acid method. The effects of sampling weight, elution velocity, and elution concentration gradient on the separation efficiency were examined and three isolated peaks were obtained. The optimal separation conditions are 10 mg of the sampling weight (sampling volume is 20 mL), 1.0 mL/min of the elution velocity, and 1.00 mol/L 2 of NaCl gradient elution. The adjacent peak resolutions among the three main components (1, 2, and 3 according to their elution order) are 0.660 (o 12 ) and 0.786 (o 23 ), respectively. It is deduced that 39.8 cm of the bed length is required for the fully separation of the three polysaccharides. © KSBB hÉóïçêÇëW=ê~Çá~ä=Ñäçï=ÅÜêçã~íçÖê~éÜóI=éìêáÑáÅ~íáçåI=Noscoc flagelliformeI=éçäóë~ÅÅÜ~êáÇÉ

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Section: Introductionmentioning

confidence: 99%

Study on the purification of polysaccharides from Noscoc flagelliforme with radial flow chromatography

Dai

Wang

Jia

et al. 2009

Biotechnol Bioproc E

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“…This velocity affects axial dispersion coefficient D b and film mass transfer coefficient k values. Meanwhile, the intraparticle diffusivities D p can be regarded as independent of v [4]. If molecular diffusivity contribution to axial dispersion is negligible, which is often true, Eq.…”

Section: Effect Of Flow Ratementioning

confidence: 99%

Mass Transfer Effects in Liquid Chromatography Simulation

2015

Mathematical Modeling and Scale-Up of Liquid Chromatography

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For analytical and some small preparative columns in LC, mass transfer resistance is usually negligible and the equilibrium theory suffices [1]. However, for preparative columns with smaller plate numbers and large-scale columns, mass transfer effects are often significant and usually cannot be neglected. The study of mass transfer effects for single-component systems has been carried out by many researchers [2]. For example, Lee et al. [3] studied the mass transfer effects in nonlinear multicomponent elution ion-exchange chromatography. They discussed the differences between a general rate model and the equilibrium theory under various mass transfer conditions.

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“…where C A is solute concentration in the resin interstices, t is time, 1 R is external void fraction of the chromatographic media, C RA is the overall concentration of solute in the resin, r is radius, D Ar and D Au are the radial and angular dispersion coefficients respectively, u is angle in radians, Q is flowrate in the radial direction, and A r is radial column area perpendicular to flow direction (Gu et al, 1991;Tsaur and Shallcross, 1997). Assumptions implicit in this equation are that the annulus is uniformly packed and liquid and solid phases are incompressible, velocity is constant with height (axial) and angular position, concentration is constant with height so axial dispersion is negligible, and temperature is constant throughout.…”

Section: Theorymentioning

confidence: 99%

“…Assumptions implicit in this equation are that the annulus is uniformly packed and liquid and solid phases are incompressible, velocity is constant with height (axial) and angular position, concentration is constant with height so axial dispersion is negligible, and temperature is constant throughout. Relations for D Ar and D Au can be found in Gu et al (1991) and Thiele et al (2001). When modelling rotating axial flow annular beds, continuity equations for axial flow columns are adapted using the relation from Wankat (1977),…”

Section: Theorymentioning

confidence: 99%

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Continuous Radial Flow Chromatography of Proteins

Lay

Fee

Swan

2006

Food and Bioproducts Processing

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A continuous radial flow chromatography (CRFC) system consisting of an annular packed bed between two rotating concentric porous stainless steel cylinders was constructed and tested. The protein feed, wash buffer and elution buffer are applied simultaneously at different fixed angular positions at the periphery of the rotating bed and flow radially inwards. The target protein is bound in the feed zone and eluted in the elution zone while unbound protein flows through the feed zone. Continuity equations for fixed bed radial flow chromatography were extended to model the CRFC by including angular displacement terms, and solved using finite difference methods. Film diffusion at the resin particle boundary and pore diffusion within the resin particle was described by an overall mass transfer coefficient and a multicomponent Langmuir isotherm was used to describe protein absorption onto the resin particle. To verify the model, a solution containing 0.53 mg ml 21 lactoferrin and 1.6 mg ml 21 bovine serum albumin (BSA) was applied at 5 ml min 21 to a 220-ml CRFC system packed with DEAE Sepharose Fast Flow ion exchange resin, with abed speed of 0.02 rpm. The proteins were separated into lactoferrin of 68% purity (74% recovery) and BSA of 94% purity (85% recovery). Experimental data were used to find values for the parameters in the model proposed.

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A theoretical study of multicomponent radial flow chromatography

Cited by 30 publications

References 22 publications

Study on the purification of polysaccharides from Noscoc flagelliforme with radial flow chromatography

Study on the purification of polysaccharides from Noscoc flagelliforme with radial flow chromatography

Mass Transfer Effects in Liquid Chromatography Simulation

Continuous Radial Flow Chromatography of Proteins

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