A thermostable humic acid peroxidase from Streptomyces sp. strain AH4: Purification and biochemical characterization

Purification and biochemical characterization of two detergent-stable serine alkaline proteases from Streptomyces sp. strain AH4

“…Nineteen actinomycete strains, which were previously isolated for their ability to degrade soil humic acids (Badis et al 2010;Fodil et al 2012), and ten newly isolated strains were determined to be protease producers, forming clear zones on casein-containing medium at pH 9.0. The ratio of the diameter of the clear zone and that of the colony served as an index for the selection of strains with high protease production.…”

“…In the present study, two extracellular proteases produced by Streptomyces sp. strain AH4, which was previously isolated from the surface soils of the Mitidja plain, Southwest of Algeria (Badis et al 2010;Fodil et al 2012), were screened for their ability to hydrolyze casein onto skimmed milk agar plates. The culture supernatant containing extracellular proteases was used as the crude enzyme preparation.…”

“…Based on physiological and biochemical assays and 16S rRNA gene sequencing, the AH4 strain was previous identified in a recent study by the authors as Streptomyces sp. (GenBank accession number: GU434674), (Badis et al 2010;Fodil et al 2012).…”

“…strain AH4, which was previously isolated from the surface soils of the Mitidja plain (Algeria) and reported to be an attractive producer of an extracellular thermostable humic acid peroxidase (Badis et al 2010;Fodil et al 2012). Both proteases were also assessed for their potential use as bioadditives in detergent formulations.…”

Characterization of a purified decolorizing detergent-stable peroxidase from Streptomyces griseosporeus SN9

“…Commercially enzymes are often reported to http://dx.doi.org/10.1016/j.ijbiomac.2014. 11.021 0141-8130/© 2014 Elsevier B.V. All rights reserved. be stable at conditions of elevated temperatures and pH.…”