A Thinopyrum intermedium chromosome in bread wheat cultivars as a source of genes conferring resistance to fungal diseases

Салина, Е. А.; Адонина, И. Г.; Бадаева, Е. Д.; Kroupin, Pavel Yu.; Stasyuk, A I; Леонова, И. Н.; Шишкіна, А. А.; Divashuk, Mikhail G.; Starikova, Elizaveta V.; Khuat, Thi Mai Luong; Syukov, V. V.; Карлов, Г. И.

doi:10.1007/s10681-014-1344-5

Cited by 55 publications

(46 citation statements)

References 25 publications

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“…The PLUG markers selected by us and allowing for amplifying the specific fragments can be used for assessment of segregating populations in order to find an alien introgression associated with plant resistance. Earlier, we demonstrated the efficiency of such an approach using PLUG markers in studying 6Ai#2(6D) substitution which determines resistance to leaf rust in the Tulaykovskaya 10 bread wheat variety [16].…”

Section: Variety Testingmentioning

confidence: 99%

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PLUG MARKERS TO DETECT ALIEN GENETIC MATERIAL IN BREAD WHEAT(Triticum aestivum L.) HYBRIDS DURING BREEDING

Sokolov¹,

Polkhovskiy²,

Kroupin³

et al. 2017

S-h. biol.

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A b s t r a c t Molecular markers make it possible to monitor the directional introgression of alien genetic material to the genome of wheat. Among the diverse molecular markers, the PLUG (PCR-based landmark unique gene) markers have the greatest advantage for analyzing distant hybrids due to the ability to amplify polymorphism fragments on closely related species and to detect simultaneously three genomes within one homoeological group in one PCR. A collection of 41 entries of different breeding stages developed in Russian State Agrarian University as a result of crossing of bread wheat (Triticum aestivum L.) and wheat T. timopheevii Zhuk. was studied. All hybrids were resistant to leaf rust and powedery mildew natural infection. Using this collection of distant hybrids, we demonstrated for the first time the possibilities of PLUG markers for identifying alien introgressions in the genome of bread wheat from T. timopheevii. In order to increase the degree of polymorphism between the entries, we additionally processed the products of amplification of PLUG markers with highfrequency restriction endonucleases TaqI and HaeIII. The entries were found to carry both single and multiple introgressions. The latter entries with introgressions in more than one homeological group are of particular interest. The heterogeneity of not only the late generations of entries, but also the entries from the nursery of the preliminary cultivar estimation, which may be associated with secondary chasmogamy, is shown. As a result of the studies, 14 PLUG markers for the long and short chromosome arm of each homeological group have been selected, which can be recommended for the detection of the genetic material of T. timopheevii in distant hybrids of T. timopheevii ½ T. aestivum. The advantages of PLUG markers as a tool for monitoring the transfer of alien hereditary material into the genome of bread wheat are discussed.Keywords: common wheat, Triticum aestivum, Triticum timopheevii, wide hybridization, molecular markers, PLUG, PCR The predominance of bread wheat (Triticum aestivum L., A u BD) over other wheat species is determined by ecological plasticity, resistance to low and high temperatures, excess and shortage of moisture, diseases and pests. Currently, this is the main food crop for about one third of the world's population [1,2]. However, in connection with impoverishment of genetic resources of bread wheat, evolution of pathogens, loss of efficiency of the present disease and pest resistance genes, a problem of search for new resistance genes for transfer into the T. aestivum genome at distant hybridization becomes important.As a source of such genes, relatives of bread wheat are used, in particular,

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Section: Variety Testingmentioning

confidence: 99%

“…Thinopyrum elongatum [15], Th. intermedium [16], Leymus mollis [17], Th. ponticum [18], in the distant hybrids of bread wheat.…”

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confidence: 99%

PLUG MARKERS TO DETECT ALIEN GENETIC MATERIAL IN BREAD WHEAT(Triticum aestivum L.) HYBRIDS DURING BREEDING

Sokolov¹,

Polkhovskiy²,

Kroupin³

et al. 2017

S-h. biol.

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“…С другой стороны, сорт Бийская озимая, не несущая транслокации 1RS.1BL, на обеих дендрограммах кластеризуется вместе с сор-том Филатовка с очень высоким значением бутстрепа. Сорта Тулайковская 100 и Омская 23 также группиру-ются вместе, несмотря на присутствие в геноме сорта Тулайковская 100 целой чужеродной хромосомы (6Ai от пырея), заместившей хромосому 6D (Salina et al, 2015). Даже сорт Фишт, характеризующийся множественными транслокациями от T. miguschovae, по результатам микро-сателлитного анализа, объединяется с высоким значени-ем бутстрепа с сортом Таня, у которого не обнаружено никаких значительных хромосомных перестроек, кроме транслокации 1RS.1BL.…”

Section: цитогенетический анализunclassified

Genotyping of hexaploid wheat varieties from different Russian regions

Адонина¹,

Леонова²,

Бадаева³

et al. 2016

Vestn. VOGiS

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We used molecular-genetic and molecular-cytology approaches to characterize the genomes of 20 varieties of wheat created in different regions of Russia. A molecular-genetic analysis was performed using 29 SSR-markers covering the entire genome, and 41 ISBP-markers localized on chromosome 5B. Analysis of genetic similarity based on the results of molecular genotyping showed that the winter wheat varieties form a common cluster, regardless of the origin or area of cultivation. This is primarily due to the fact that the varieties originating from the European part of Russia were used to establish winter wheat varieties for West Siberia. Comparative analysis of individual dendrograms constructed using 1-2 markers per chromosome, and with the involvement of a larger number of 5B-chromosome markers allowed us to identify varieties with rearrangements of this chromosome and to assess genetic diversity. We found that winter wheat Vassa and spring wheat Chelyaba 75 were clustered closely together. This is an indirect confi mation of the use of winter wheat varieties in the breeding to improve the productive potential of spring wheat. Molecular-cytology analysis by C-banding and fluo escence in situ hybridization (FISH) revealed various chromosomal rearrangements in 8 of 20 cultivars studied, including translocations from S. cereale, Ae. speltoides and Th. intermedium. Thus, a combination of the two approaches allowed us to better characterize genomes of wheat varieties of various origin.Key words: Triticum aestivum; winter and spring varieties; genotyping; SSR-and ISBP-markers; C-banding; fluo escence in situ hybridization (FISH).Для характеристики геномов 20 сортов мягкой пшеницы, созданных в различных регионах России, были использованы молекулярно-генетический и молекулярно-цитологический подходы. Молекулярно-генетический анализ проводился с применением 29 SSR-маркеров, охватывающих весь геном, и 41 ISBP-маркера, локализованного на хромосоме 5В. Анализ генетического сходства, проведенный на основании результатов молекулярного генотипирования, показал, что озимые пшеницы образуют общий кластер независимо от происхождения и зоны возделывания. Это, в первую очередь, объясняется тем, что при создании озимых сортов для Западно-Cибирского региона привлекались формы, происходящие из европейской части России. Сравнительный анализ индивидуальных дендрограмм, построенных на основании данных по одному-двум маркерам на каждую хромосому и с привлечением большего числа маркеров по хромосоме 5В, позволяет, помимо оценки генетического разнообразия, идентифицировать сорта, имеющие перестройки по изучаемой хромосоме. Показана кластеризация озимой пшеницы Васса с яровым сортом Челяба 75, что может быть косвенным подтверждением использования озимых форм в селекции для повышения потенциала продуктивности яровой пшеницы. В результате молекулярно-цитологического анализа методами C-бэндинга и FISH у 8 из 20 изученных сортов были выявлены различные хромосомные перестройки, в том числе интрогрессии, происходящие от S. cereale, Ae. speltoides и ...

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“…Spring forms containing alien genetic material with resistance genes inherited from Triticum timopheevii and Thinopyrum intermedium were donors [19,20]. Obtaining winter genotypes with molecular markers for identification of the genes controlling winter or spring mode of life and resistance to leaf rust diseases, and evaluating their winter hardiness and susceptibility to the pathogen have been described earlier [21].…”

Section: Introductionmentioning

confidence: 99%

“…timopheevii), and the soft wheat variety Tulaikovskaya 10 (T. aestivum/Th. Intermedium) with leaf rust resistance genes LrTt2 and Lr6Ai#2, respectively, were the donors [19,20]. Before the hybridization, the winter varieties were vernalized for 60 days at 3 С.…”

Section: Introductionmentioning

confidence: 99%

Variability of Agronomically Important Traits in Spring Wheat Hybrids Obtained by Marker-Assisted Selection From Crosses of Winter Wheat With Spring Wheat Donors of Resistance Genes

Stasyuk¹,

Леонова²,

Салина³

2017

S-h. biol.

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A b s t r a c tSpring wheat is the main cereal crop in the West Siberian region of Russia and occupies more than 40 % of the acreage. The widening of the genetic diversity of this culture and the creation of new varieties with high productivity and resistance to environmental factors have always been a key problem of breeding. Winter wheat varieties which are characterized by a wide diversity of useful characters such as tolerance to abiotic stressors, productive tillers and yield can be used as a promising source of agronomically important traits. Despite the fact that the methodological approach to the introgression of the gene pool of winter wheat into the genome of spring wheat has been in use for a long time to increase the yield of spring varieties, at the moment there is little available information concerning the manifestation of valuable traits in offspring obtained from the winter and spring wheat hybridization. However, such results are important for assessing the combining ability of winter and spring wheat varieties and for the selection of potential parents by a complex of characters. For the identification of the winter and spring genotypes in the offspring resulting from the hybridization of winter and spring parents with the help of convenient breeding methods, a large sample size populations and a long period of cultivation of plant material are required. The application of MAS technologies and markers linked to the target loci can significantly decrease the time of development of new breeding lines. In creation of breeding lines resistant to leaf rust, the winter wheat varieties Filatovka and Biyskaya ozymaya, adapted for cultivation in the West Siberian region, were involved in a scheme of hybridization. Spring wheat introgression line 5366-180 (Triticum aestivum/T. timopheevii) и spring wheat cultivar Tulaikovskaya 10, containing genetic material of Thinopyrum intermedium were the donors of effective leaf rust resistance genes LrTt2 and Lr6Ai#2. The aim of this work was to evaluate the diversity of spring wheat forms obtained from these crosses on economically important traits and analysis of the genotype influence on their manifestation. The plants differing in winter and spring habits were selected in F 2 progeny by means of allele-specific markers to the VRN-1 genes. To identify genotypes containing leaf rust resistance genes, a microsatellite marker Xbarc232 and primers MF2/MR1r2 and MF2/MR4 specific for T. timopheevii and Th. intermedium translocations, respectively, were used. As a result of hybridization of winter varieties with spring donors, 122 F 2 plants were obtained, of which 30 homozygous spring plants with different allelic composition of VRN-1 genes were selected. Of these, 12 plants according to molecular data with Lr-specific primers contained alien translocations. For the comparative analysis of agronomic traits, six F 2 plants were used which possessed the same allelic composition of VRN-1 genes as in the initial spring donors but differed in the presence or absence of Lr...

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A Thinopyrum intermedium chromosome in bread wheat cultivars as a source of genes conferring resistance to fungal diseases

Cited by 55 publications

References 25 publications

PLUG MARKERS TO DETECT ALIEN GENETIC MATERIAL IN BREAD WHEAT(Triticum aestivum L.) HYBRIDS DURING BREEDING

PLUG MARKERS TO DETECT ALIEN GENETIC MATERIAL IN BREAD WHEAT(Triticum aestivum L.) HYBRIDS DURING BREEDING

Genotyping of hexaploid wheat varieties from different Russian regions

Variability of Agronomically Important Traits in Spring Wheat Hybrids Obtained by Marker-Assisted Selection From Crosses of Winter Wheat With Spring Wheat Donors of Resistance Genes

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