A three-dimensional (3D) printed biomimetic hierarchical scaffold with a covalent modular release system for osteogenesis

Chen, Guanghua; Sun, Yi; Lu, F. Z.; Jiang, Aiting; Subedi, Dipendra; Kong, Pengyu; Wang, Xiaoyan; Yu, Tailong; Chi, Hui; Song, Chengyi; Liu, Kunyu; Qi, Pengfei; Yan, Jinglong; Ji, Yiqin

doi:10.1016/j.msec.2019.109842

Cited by 34 publications

(23 citation statements)

References 42 publications

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“…Furthermore, in vivo experimental studies have proven that 3Dprinted scaffolds made of HAp and its composites could act as excellent bone replacements, thanks to proper osteoconductivity (Cho et al, 2019b;Sha et al, 2019). It should be mentioned that 3D-printed HAp scaffolds are also found as competent vehicles to deliver osteogenic growth factors like bone morphogenetic protein-2 for improving in vivo bone regeneration (Chen G. et al, 2019).…”

Section: Potential and Significance In Hard Tissue Regeneration And Dmentioning

confidence: 99%

“…For example, macroporous HAp was used as a suitable carrier for the oral delivery of carvedilol, a poorly water-soluble drug (Zhao et al, 2011). Over time and along with the advent of 3D printing technology, controlled release of various bioactive molecules (e.g., rhBMP-2) from 3D-printed porous HAp scaffolds was highlighted as a novel DDS for bone regeneration (Wang et al, 2016;Chen G. et al, 2019).…”

Section: Potential and Significance In Hard Tissue Regeneration And Dmentioning

confidence: 99%

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Additive Manufacturing Methods for Producing Hydroxyapatite and Hydroxyapatite-Based Composite Scaffolds: A Review

et al. 2019

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Hydroxyapatite (HAp) has been considered for decades an ideal biomaterial for bone repair due to its compositional and crystallographic similarity to bioapatites in hard tissues. However, fabrication of porous HAp acting as a template (scaffold) for supporting bone regeneration and growth has been a challenge to biomaterials scientists. The introduction of additive manufacturing technologies, which provide the advantages of a relatively fast, precise, controllable, and potentially scalable fabrication process, has opened new horizons in the field of bioceramic scaffolds. This review focuses on three-dimensional-printed HAp scaffolds and related composite systems, where the calcium phosphate phase is combined with other ceramics or polymers improving the mechanical properties and/or imparting special extra-functionalities. The main applications of three-dimensional-printed HAp scaffolds in bone tissue engineering are presented and discussed; furthermore, this review also emphasizes the most recent achievements toward the development and testing of multifunctional HAp-based systems combining multiple properties for advanced therapy (e.g., bone regeneration, antibacterial effect, angiogenesis, and cancer treatment).

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Section: Potential and Significance In Hard Tissue Regeneration And Dmentioning

confidence: 99%

Section: Potential and Significance In Hard Tissue Regeneration And Dmentioning

confidence: 99%

Additive Manufacturing Methods for Producing Hydroxyapatite and Hydroxyapatite-Based Composite Scaffolds: A Review

et al. 2019

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“… 34 , 35 , 68 − 72 Advances in scaffold design methodologies have led to state-of-the-art 3D printing technologies that allow for the precise control over pore size, geometry, and distribution, permitting the design of interconnected porous networks that facilitate cell attachment and increase mass transport of oxygen and nutrients throughout the construct. 6 , 18 , 30 , 45 , 68 , 73 − 79 In particular, stereolithography (SLA) has been used to produce ceramic bone substitutes; however, this process requires the use of support features for overhanging or fragile parts and removal of these structures can introduce fracture tips and microcracks, which can propagate and weaken the construct. 72 , 80 − 84 In overcoming these challenges, SEPS, an advanced SLA printing technique, has been developed to produce complex ceramic scaffolds with increased resolution, higher densities, and greater geometric fidelity.…”

Section: Discussionmentioning

confidence: 99%

Rat Calvarial Bone Regeneration by 3D-Printed β-Tricalcium Phosphate Incorporating MicroRNA-200c

Remy

Akkouch

et al. 2021

ACS Biomater. Sci. Eng.

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Advanced fabrication methods for bone grafts designed to match defect sites that combine biodegradable, osteoconductive materials with potent, osteoinductive biologics would significantly impact the clinical treatment of large bone defects. In this study, we engineered synthetic bone grafts using a hybrid approach that combined three-dimensional (3D-)printed biodegradable, osteoconductive β-tricalcium phosphate (β-TCP) with osteoinductive microRNA(miR)-200c. 3D-printed β-TCP scaffolds were fabricated utilizing a suspension-enclosing projection-stereolithography (SEPS) process to produce constructs with reproducible microarchitectures that enhanced the osteoconductive properties of β-TCP. Collagen coating on 3D-printed β-TCP scaffolds slowed the release of plasmid DNA encoding miR-200c compared to noncoated constructs. 3D-printed β-TCP scaffolds coated with miR-200c -incorporated collagen increased the transfection efficiency of miR-200c of both rat and human BMSCs and additionally increased osteogenic differentiation of hBMSCs in vitro . Furthermore, miR-200c -incorporated scaffolds significantly enhanced bone regeneration in critical-sized rat calvarial defects. These results strongly indicate that bone grafts combining SEPS 3D-printed osteoconductive biomaterial-based scaffolds with osteoinductive miR-200c can be used as superior bone substitutes for the clinical treatment of large bone defects.

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“…Other recent examples have demonstrated, in vivo, successful calvarial bone regeneration using printed scaffolds made of hydroxyapatite (HA) or PCL/PLGA/HA composite, respectively [111,112]. Furthermore, the advantage of printing techniques to process multiple bioinks in a single scaffold was used to bioactivate the scaffold with BMP-2 peptide or µ-RiboNucleic Acid (µ-RNA) conjugates to enhance stem cells’ osteoinduction to stimulate in vivo bone formation.…”

Section: Layer-by-layer Approaches For Scaffolds’ Fabricationmentioning

confidence: 99%

Modular Strategies to Build Cell-Free and Cell-Laden Scaffolds towards Bioengineered Tissues and Organs

Salerno

Cesarelli

Pedram

et al. 2019

JCM

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Engineering three-dimensional (3D) scaffolds for functional tissue and organ regeneration is a major challenge of the tissue engineering (TE) community. Great progress has been made in developing scaffolds to support cells in 3D, and to date, several implantable scaffolds are available for treating damaged and dysfunctional tissues, such as bone, osteochondral, cardiac and nerve. However, recapitulating the complex extracellular matrix (ECM) functions of native tissues is far from being achieved in synthetic scaffolds. Modular TE is an intriguing approach that aims to design and fabricate ECM-mimicking scaffolds by the bottom-up assembly of building blocks with specific composition, morphology and structural properties. This review provides an overview of the main strategies to build synthetic TE scaffolds through bioactive modules assembly and classifies them into two distinct schemes based on microparticles (µPs) or patterned layers. The µPs-based processes section starts describing novel techniques for creating polymeric µPs with desired composition, morphology, size and shape. Later, the discussion focuses on µPs-based scaffolds design principles and processes. In particular, starting from random µPs assembly, we will move to advanced µPs structuring processes, focusing our attention on technological and engineering aspects related to cell-free and cell-laden strategies. The second part of this review article illustrates layer-by-layer modular scaffolds fabrication based on discontinuous, where layers’ fabrication and assembly are split, and continuous processes.

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A three-dimensional (3D) printed biomimetic hierarchical scaffold with a covalent modular release system for osteogenesis

Cited by 34 publications

References 42 publications

Additive Manufacturing Methods for Producing Hydroxyapatite and Hydroxyapatite-Based Composite Scaffolds: A Review

Additive Manufacturing Methods for Producing Hydroxyapatite and Hydroxyapatite-Based Composite Scaffolds: A Review

Rat Calvarial Bone Regeneration by 3D-Printed β-Tricalcium Phosphate Incorporating MicroRNA-200c

Modular Strategies to Build Cell-Free and Cell-Laden Scaffolds towards Bioengineered Tissues and Organs

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