The root canal and root morphologies of maxillary second molars might vary. This article details the endodontic treatment of two cases involving unusual root canal morphology in a maxillary second molar. The first case involved a single root and a single canal, whereas the second case had a single root with two separate canals. A literature review was conducted to clarify the prevalence of anatomical changes in the number of roots and canals in the maxillary second molar. The utilisation of a dental operating microscope facilitated the detection of the canal orifices in both cases. The objective of this study is to increase the knowledge of clinicians regarding the atypical structure of root canals by examining the complex differences found in maxillary second molars.