A three-dimensional and bevel-angled ultrahigh aspect ratio microneedle for minimally invasive and painless blood sampling

Lee, Dae Sik; Li, Cheng Guo; Ihm, Chunhwa; Jung, Hyungil

doi:10.1016/j.snb.2017.08.030

Cited by 35 publications

(17 citation statements)

References 28 publications

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“…Figure 1F is the optical microscopic image of the GelMA MN patch, which showed an 11 × 11 array of uniformly aligned MNs fixed on a 1 cm × 1 cm patch to support sufficient ISF extraction for later analysis. The most commonly used techniques to manufacture MNs with different shapes and sizes include micromolding,30 lithography,31 laser cutting,32 3D printing,33 and wet or dry etching 34. In the present work, we used a polydimethylsiloxane (PDMS) mold with a predesigned shape and size to manufacture the MN patches.…”

Section: Figurementioning

confidence: 99%

Gelatin Methacryloyl Microneedle Patches for Minimally Invasive Extraction of Skin Interstitial Fluid

Zhu

Zhou

Kim

et al. 2020

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The extraction of interstitial fluid (ISF) from skin using microneedles (MNs) has attracted growing interest in recent years due to its potential for minimally invasive diagnostics and biosensors. ISF collection by absorption into a hydrogel MN patch is a promising way that requires the materials to have outstanding swelling ability. Here, a gelatin methacryloyl (GelMA) patch is developed with an 11 × 11 array of MNs for minimally invasive sampling of ISF. The properties of the patch can be tuned by altering the concentration of the GelMA prepolymer and the crosslinking time; patches are created with swelling ratios between 293% and 423% and compressive moduli between 3.34 MPa and 7.23 MPa. The optimized GelMA MN patch demonstrates efficient extraction of ISF. Furthermore, it efficiently and quantitatively detects glucose and vancomycin in ISF in an in vivo study. This minimally invasive approach of extracting ISF with a GelMA MN patch has the potential to complement blood sampling for the monitoring of target molecules from patients.

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Section: Figurementioning

confidence: 99%

Gelatin Methacryloyl Microneedle Patches for Minimally Invasive Extraction of Skin Interstitial Fluid

Zhu

Zhou

Kim

et al. 2020

Small

141

130

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“…The hollow MNs extract ISF or blood into their bores by a capillary action or external negative pressure. As the hollow MNs were put to use as micro injection needles for localized drug delivery into the skin at the initial stage of the development of research in MNs [ 3 , 75 ], the methods of fabrication and theoretical analysis of the hollow MNs are well investigated [ 4 , 76 – 81 ]. As a result, the hollow MNs for fluid extraction are mainly made of Si or metals using MEMS-based micromachining technologies, including chemical etching or electroplating.…”

Section: Mns As a Biological Fluid Collectorsmentioning

confidence: 99%

Functionalized microneedles for continuous glucose monitoring

Takeuchi

Kim

2018

Nano Convergence

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Microneedles (MNs) have been established as promising medical devices as they are minimally invasive, cause less pain, and can be utilized for self-administration of drugs by patients. There has been rapid development in MNs for transdermal monitoring and diagnostic systems, following the active research on fabrication methods and applications for drug delivery. In this paper, recent investigations on bio-sensing using MNs are reviewed in terms of the applicability to continuous glucose monitoring system (CGMS), which is one of the main research focuses of medical engineering technologies. The trend of the functionalized MNs can be categorized as follows: (i) as a sensing probe, and (ii) as a biological fluid collector. MNs as in vivo sensors are mainly integrated or coated with conductive materials to have the function as electrodes. MNs as fluid collectors are given a certain geometrical design, such as a hollow and porous structure aided by a capillary action or negative pressure, to extract the interstitial fluids or blood for ex vivo analysis. For realization of CGMS with MNs, a long-term accurate measurement by the MN-based sensing probe or a fluidic connection between the MN-based fluid collector and the existing microfluidic measurement systems should be investigated.

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“…A synopsis is presented in Table S4 , with reference to geometries in MN studies presented in Tables S1–S3 . MN structures range over the following spatial configurations: Pointed tip with triangular slope [ 54 ], conical shape [ 2 , 4 , 14 , 55 , 56 ], cylindrical shape [ 57 ], cylindrical body and pointed head [ 45 , 52 , 58 ], tip-beveled triangular cylinder shape [ 34 ], tip-beveled cylinder shape [ 59 , 60 ], pyramidal shape [ 8 , 12 , 61 ], and tetrahedron-shaped [ 62 ]. Based on the studies, these different microneedle shapes and geometries have been shown to breach the stratum corneum layer of human and porcine skin.…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional (3D) Printed Microneedles for Microencapsulated Cell Extrusion

et al. 2018

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Cell-hydrogel based therapies offer great promise for wound healing. The specific aim of this study was to assess the viability of human hepatocellular carcinoma (HepG2) cells immobilized in atomized alginate capsules (3.5% (w/v) alginate, d = 225 µm ± 24.5 µm) post-extrusion through a three-dimensional (3D) printed methacrylate-based custom hollow microneedle assembly (circular array of 13 conical frusta) fabricated using stereolithography. With a jetting reliability of 80%, the solvent-sterilized device with a root mean square roughness of 158 nm at the extrusion nozzle tip (d = 325 μm) was operated at a flowrate of 12 mL/min. There was no significant difference between the viability of the sheared and control samples for extrusion times of 2 h (p = 0.14, α = 0.05) and 24 h (p = 0.5, α = 0.05) post-atomization. Factoring the increase in extrusion yield from 21.2% to 56.4% attributed to hydrogel bioerosion quantifiable by a loss in resilience from 5470 (J/m3) to 3250 (J/m3), there was no significant difference in percentage relative payload (p = 0.2628, α = 0.05) when extrusion occurred 24 h (12.2 ± 4.9%) when compared to 2 h (9.9 ± 2.8%) post-atomization. Results from this paper highlight the feasibility of encapsulated cell extrusion, specifically protection from shear, through a hollow microneedle assembly reported for the first time in literature.

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A three-dimensional and bevel-angled ultrahigh aspect ratio microneedle for minimally invasive and painless blood sampling

Cited by 35 publications

References 28 publications

Gelatin Methacryloyl Microneedle Patches for Minimally Invasive Extraction of Skin Interstitial Fluid

Gelatin Methacryloyl Microneedle Patches for Minimally Invasive Extraction of Skin Interstitial Fluid

Functionalized microneedles for continuous glucose monitoring

Three-Dimensional (3D) Printed Microneedles for Microencapsulated Cell Extrusion

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