A three-dimensional model of fluid–structural interactions for quantifying the contractile force for cardiomyocytes on hybrid biopolymer microcantilever

Park, Jungyul; Ryu, Suk Kyu; Kim, Jinseok; Cha, Junghun; Baek, Jeongeun; Park, Sukho; Kim, Byung Kyu; Lee, Sang Ho

doi:10.1016/j.jbiomech.2007.03.026

Cited by 8 publications

(6 citation statements)

References 27 publications

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“…In addition, because there exist different effects of cell adhesion and locomotion compared with a typical flat surface, the reliability of the obtained experimental result is insufficient together with a relatively small displacement from a single-cell level. 15) In contrast, the cantilever method measures a macroscopic bending of the microcantilever, which is caused by a contractile behavior of the CMs in three-dimensional structure with reconstituted cell-to-cell connections and synchronized tissue-like behavior. 16,17) In this manner, it can usually yield a larger displacement with an improved ease of use.…”

Section: Introductionmentioning

confidence: 99%

Numerical investigation of perforated polymer microcantilever sensor for contractile behavior of cardiomyocytes

Nguyen

Lee

2017

Jpn. J. Appl. Phys.

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In this study, a numerical investigation of microcantilever sensors for detecting the contractile behavior of cardiomyocytes (CMs) was performed. Recently, a novel surface-patterned perforated SU-8 microcantilever sensor has been developed for the preliminary screening of cardiac toxicity. From the contractile motion of the CMs cultured on the microcantilever surface, a macroscopic bending of the microcantilever was obtained, which is considered to reflect a physiological change. As a continuation of the previous research, a novel numerical method based on a surface traction model was proposed and verified to further understand the bending behavior of the microcantilevers. Effects of various factors, including surface traction magnitude, focal area of CMs, and stiffness of microcantilever, on the bending displacement were investigated. From static and transient analyses, the focal area was found to be the most crucial factor. In addition, the current result can provide a design guideline for various micromechanical devices based on the same principle.

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Section: Introductionmentioning

confidence: 99%

Numerical investigation of perforated polymer microcantilever sensor for contractile behavior of cardiomyocytes

Nguyen

Lee

2017

Jpn. J. Appl. Phys.

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“…The integration of muscle cells on grooved structured cantilever were found to generate more bending than on the flat surfaces as shown in Fig. (10) [70][71][72]. The forces generated by the living cells on the microcantilever structures have been harnessed to create cell-powered mechanical motors [73].…”

Section: Biosensing Applicationsmentioning

confidence: 99%

Microcantilever Biosensors: Probing Biomolecular Interactions at the Nanoscale

Li¹,

Shu

2011

COC

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Microcantilever based biosensors have attracted much attention as a label-free, real-time and highly sensitive approach to the detection of biomolecules. When specific biomolecular interactions occur between a receptor immobilized on one side of a cantilever and a target analyte in solution, a mechanical bending of the cantilever results due to a change in surface stress, thereby translating biochemical interactions into a concentration-dependent nanomechanical response of the microcantilevers. In this review, we present a general overview of the operation principles, fabrication and preparation of microcantilever biosensors and discuss their uses for a wide range of biosensing applications. The focus of the review is given to probing the biomolecular interactions at the solid-liquid interface by measuring the cantilever bending. In addition to the label-free detection of DNA, protein and cells, the emphasis is also made to discuss about the new development of microcantilever biosensors for label-free probing nanoscale conformational changes of DNA, protein and polymers, and real-time detection of nanomechanical forces from living cells. Finally, the outlook for future development work and challenges is also discussed.

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“…As a consequence, a change in contractility of the CMs can produce macroscopic bending behavior in the microcantilever [7][8][9]. Compared with the developed micropillar structure [10][11][12], the microcantilever approach does not need an optical microscopy image analysis and can easily be achieved by real-time analysis [13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

“…Nguyen et al reported a perforated microcantilever that could decrease the microcantilever's stiffness using a finite element method (FEM) simulation [18]. While some attempts have been made by researchers [11][12][13][14][15][16][17][18][19][20][21], the relationship between the contractile force generated by the CMs and the subsequent macroscopic behavior of a micro-mechanical device has not been investigated. Thus, further research needs to be performed on the influence of the structural parameters (such as porosity factor) on the bending effect of a perforated microcantilever.…”

Section: Introductionmentioning

confidence: 99%

A Numerical Model of a Perforated Microcantilever Covered with Cardiomyocytes to Improve the Performance of the Microcantilever Sensor

Qiu

et al. 2020

Materials

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A few simple polymeric microsystems, such as microcantilever sensors, have recently been developed for the preliminary screening of cardiac toxicity. The microcantilever deflection produced by a change in the cardiomyocyte (CM) contraction force is important for understanding the mechanism of heart failure. In this study, a new numerical model is proposed to analyze the contractile behavior of CMs cultured on a perforated microcantilever surface for improving the performance of the microcantilever sensor. First, the surface traction model is used to investigate the bending displacement of the plain microcantilever. In order to improve the bending effect, a new numerical model is developed to analyze the bending behavior of the perforated microcantilever covered with CMs. Compared with the designed molds, the latter yields better results. Finally, a simulation analysis is proposed based on a finite element method to verify the presence of a preformed mold. Moreover, the effects of various factors on the bending displacement, including microcantilever size, Young’s modulus, and porosity factor, are investigated. Both the simulation and numerical results have good consistency, and the maximum error between the numerical and simulation results is not more than 3.4%, even though the porosity factor reaches 0.147. The results show that the developed mold opens new avenues for CM microcantilever sensors to detect cardiac toxicity.

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A three-dimensional model of fluid–structural interactions for quantifying the contractile force for cardiomyocytes on hybrid biopolymer microcantilever

Cited by 8 publications

References 27 publications

Numerical investigation of perforated polymer microcantilever sensor for contractile behavior of cardiomyocytes

Numerical investigation of perforated polymer microcantilever sensor for contractile behavior of cardiomyocytes

Microcantilever Biosensors: Probing Biomolecular Interactions at the Nanoscale

A Numerical Model of a Perforated Microcantilever Covered with Cardiomyocytes to Improve the Performance of the Microcantilever Sensor

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