A three-dimensional multi-electrode array for multi-site stimulation and recording in acute brain slices

Heuschkel, Marc Olivier; Fejtl, Michael; Raggenbass, Mario; Bertrand, Daniel; Renaud, Philippe

doi:10.1016/s0165-0270(01)00514-3

Cited by 258 publications

(210 citation statements)

References 11 publications

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“…This method resulted in a much higher recorded impedance than that of the gold traces themselves, due to the gold/HBSS and HBSS/wire interfacial resistance; regardless, this was an effective method for evaluating conductivity without damaging electrode exposures through rough contact with spectrum analyzer leads. Results for impedance testing of the electrode contacts and insulated wire traces are given in Figure 5, and show that the electrode impedances are uniform across electrodes and compare favorably to properties of rigid-MEA electrodes (Heuschkel et al, 2002;Oka et al, 1999). Impedance measurement results also indicate that the PDMS insulating layer acts well as an insulator ( Figure 5A).…”

Section: Electro-mechanical Testingmentioning

confidence: 86%

A lithographically-patterned, elastic multi-electrode array for surface stimulation of the spinal cord

Meacham

Giuly

Guo

et al. 2007

Biomed Microdevices

101

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A new, scalable process for microfabrication of a silicone-based, elastic multi-electrode array (MEA) is presented. The device is constructed by spinning poly(dimethylsiloxane) (PDMS) silicone elastomer onto a glass slide, depositing and patterning gold to construct wires and electrodes, spinning on a second PDMS layer, and then micropatterning the second PDMS layer to expose electrode contacts. The micropatterning of PDMS involves a custom reactive ion etch (RIE) process that preserves the underlying gold thin film. Once completed, the device can be removed from the glass slide for conformal interfacing with neural tissue. Prototype MEAs feature electrodes smaller than those known to be reported on silicone substrate (60 μm diameter exposed electrode area) and were capable of selectively stimulating the surface of the in vitro isolated spinal cord of the juvenile rat. Stretchable serpentine traces were also incorporated into the functional PDMS-based MEA, and their implementation and testing is described.

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Section: Electro-mechanical Testingmentioning

confidence: 86%

A lithographically-patterned, elastic multi-electrode array for surface stimulation of the spinal cord

Meacham

Giuly

Guo

et al. 2007

Biomed Microdevices

101

View full text Add to dashboard Cite

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“…However, the latter becomes more useful in studies on synaptic plasticity due to the following advan- [24] . Heuschkel et al [25] later applied this 3D MEA to acute hippocampal slices and obtained better …”

Section: Stable Field Excitatory Postsynaptic Potentials (Fepsps)mentioning

confidence: 99%

Use of multi-electrode array recordings in studies of network synaptic plasticity in both time and space

Liu

Chen

et al. 2012

Neurosci. Bull.

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Simultaneous multisite recording using multi-electrode arrays (MEAs) in cultured and acutely-dissociated brain slices and other tissues is an emerging technique in the field of network electrophysiology. Over the past 40 years, great efforts have been made by both scientists and commercial concerns, to advance this technique. The MEA technique has been widely applied to many regions of the brain, retina, heart and smooth muscle in various studies at the network level.The present review starts from the development of MEA techniques and their uses in brain preparations, and then specifically concentrates on the use of MEA recordings in studies of synaptic plasticity at the network level in both the temporal and spatial domains. Because the MEA technique helps bridge the gap between single-cell recordings and behavioral assays, its wide application will undoubtedly shed light on the mechanisms underlying brain functions and dysfunctions at the network level that remained largely unknown due to the technical difficulties before it matured.

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“…However, the extracellular stimulation from the array suffers from poor spatial localization (Heuschkel et al, 2002) and stimulation artifacts (Wagenaar and Potter, 2004). These stimulation issues have been circumvented by using optical stimulation of caged glutamate to stimulate neurons, while recording exracellular signals via the MEA (Ghezzi et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Laser-evoked synaptic transmission in cultured hippocampal neurons expressing channelrhodopsin-2 delivered by adeno-associated virus

Wang¹,

Hasan²,

Seung³

2009

Journal of Neuroscience Methods

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We present a method for studying synaptic transmission in mass cultures of dissociated hippocampal neurons based on patch clamp recording combined with laser stimulation of neurons expressing Channelrhodopsin-2 (ChR2). Our goal was to use the high spatial resolution of laser illumination to come as close as possible to the ideal of identifying monosynaptically coupled pairs of neurons, which is conventionally done using microisland rather than mass cultures. Using recombinant adenoassociated virus (rAAV) to deliver the ChR2 gene, we focused on the time period between 14 and 20 days in vitro, during which expression levels are high, and spontaneous bursting activity has not yet started. Stimulation by wide-field illumination is sufficient to make the majority of ChR2-expressing neurons spike. Stimulation with a laser spot at least 10 μm in diameter also produces action potentials, but in a reduced fraction of neurons. We studied synaptic transmission by voltageclamping a neuron with low expression of ChR2 and scanning a 40 μm laser spot at surrounding locations. Responses were observed to stimulation at a subset of locations in the culture, indicating spatial localization of stimulation. Pharmacological means were used to identify responses that were synaptic. Many responses were of smaller amplitude than those typically found in microisland cultures. We were unable to find an entirely reliable criterion for distinguishing between monosynaptic and polysynaptic responses. However, we propose that postsynaptic currents with Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. HHMI Author ManuscriptHHMI Author Manuscript HHMI Author Manuscript small amplitudes, simple shapes, and latencies not much greater than 8 msec are reasonable candidates for monosynaptic interactions.

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A three-dimensional multi-electrode array for multi-site stimulation and recording in acute brain slices

Cited by 258 publications

References 11 publications

A lithographically-patterned, elastic multi-electrode array for surface stimulation of the spinal cord

A lithographically-patterned, elastic multi-electrode array for surface stimulation of the spinal cord

Use of multi-electrode array recordings in studies of network synaptic plasticity in both time and space

Laser-evoked synaptic transmission in cultured hippocampal neurons expressing channelrhodopsin-2 delivered by adeno-associated virus

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