A three-locus model for the chicken major histocompatibility complex

Pink, J. R. L.; Droege, W.; Hála, K; Miggiano, V.; Ziegler, Andreas

doi:10.1007/bf01570477

Cited by 206 publications

(92 citation statements)

References 27 publications

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“…chicken MHC is simpler and smaller, with the genes arranged differently (1)(2)(3)(4)(5). Moreover, the chicken MHC can determine decisive resistance and susceptibility to several infectious pathogens, as well as response to both live and killed commercial vaccines (6)(7)(8)(9).…”

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confidence: 99%

Peptide motifs of the single dominantly expressed class I molecule explain the striking MHC-determined response to Rous sarcoma virus in chickens

Wallny

Ávila

Hunt

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

172

243

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Compared with the MHC of typical mammals, the chicken MHC is smaller and simpler, with only two class I genes found in the B12 haplotype. We make five points to show that there is a singledominantly expressed class I molecule that can have a strong effect on MHC function. First, we find only one cDNA for two MHC haplotypes (B14 and B15) and cDNAs corresponding to two genes for the other six (B2, B4, B6, B12, B19, and B21). Second, we find, for the B4, B12, and B15 haplotypes, that one cDNA is at least 10-fold more abundant than the other. Third, we use 2D gel electrophoresis of class I molecules from pulse-labeled cells to show that there is only one heavy chain spot for the B4 and B15 haplotypes, and one major spot for the B12 haplotype. Fourth, we determine the peptide motifs for B4, B12, and B15 cells in detail, including pool sequences and individual peptides, and show that the motifs are consistent with the peptides binding to models of the class I molecule encoded by the abundant cDNA. Finally, having shown for three haplotypes that there is a single dominantly expressed class I molecule at the level of RNA, protein, and antigenic peptide, we show that the motifs can explain the striking MHC-determined resistance and susceptibility to Rous sarcoma virus. These results are consistent with the concept of a ''minimal essential MHC'' for chickens, in strong contrast to typical mammals.antigen presentation ͉ avian ͉ essential ͉ evolution ͉ minimal

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mentioning

confidence: 99%

Peptide motifs of the single dominantly expressed class I molecule explain the striking MHC-determined response to Rous sarcoma virus in chickens

Wallny

Ávila

Hunt

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

172

243

View full text Add to dashboard Cite

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“…Among the genetic loci potentially involved in the immune response, the chicken MHC can determine decisive resistance and susceptibility to several infectious pathogens, as well as the response to both live and killed commercial vaccines (1)(2)(3)(4). Compared with typical mammals such as humans and mice, the chicken MHC is simpler, smaller, and rearranged (5)(6)(7)(8). Based on these data, we developed the concept of a "minimal essential MHC" to give a molecular basis to the striking functional properties of the chicken MHC (7)(8)(9).…”

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confidence: 99%

Different Evolutionary Histories of the Two Classical Class I Genes BF1 and BF2 Illustrate Drift and Selection within the Stable MHC Haplotypes of Chickens

Shaw

Powell

Marston

et al. 2007

The Journal of Immunology

108

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Compared with the MHC of typical mammals, the chicken MHC (BF/BL region) of the B12 haplotype is smaller, simpler, and rearranged, with two classical class I genes of which only one is highly expressed. In this study, we describe the development of long-distance PCR to amplify some or all of each class I gene separately, allowing us to make the following points. First, six other haplotypes have the same genomic organization as B12, with a poorly expressed (minor) BF1 gene between DMB2 and TAP2 and a well-expressed (major) BF2 gene between TAP2 and C4. Second, the expression of the BF1 gene is crippled in three different ways in these haplotypes: enhancer A deletion (B12, B19), enhancer A divergence and transcription start site deletion (B2, B4, B21), and insertion/rearrangement leading to pseudogenes (B14, B15). Third, the three kinds of alterations in the BF1 gene correspond to dendrograms of the BF1 and poorly expressed class II B (BLB1) genes reflecting mostly neutral changes, while the dendrograms of the BF2 and well-expressed class II (BLB2) genes each have completely different topologies reflecting selection. The common pattern for the poorly expressed genes reflects the fact the BF/BL region undergoes little recombination and allows us to propose a pattern of descent for these chicken MHC haplotypes from a common ancestor. Taken together, these data explain how stable MHC haplotypes predominantly express a single class I molecule, which in turn leads to striking associations of the chicken MHC with resistance to infectious pathogens and response to vaccines.

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“…The difference in the L blood group antigens found between the GSP (B 21 ) and GSN/1 (B 21 ) inbred lines did not affect the shared homogeneity in relation to the B alleles because it was not associated with the histocompatibility complex of the chicken [32]. The B-G antigens have no function in alloreaction such as skingraft, graft versus host reaction (GVHR) or MLR [28,31,36]. Nevertheless, the B-G region is considered to be included in the chicken MHC because of its close structural linkage to the B-L/B-F regions in the MHC (Fig.…”

Section: Discussionmentioning

confidence: 99%

Histocompatible Chicken Inbred Lines: Homogeneities in the Major Histocompatibility Complex Antigens of the GSP, GSN/1, PNP/DO and BM-C Inbred Lines Assessed by Hemagglutination, Mixed Lymphocyte Reaction and Skin Transplantation

et al. 2007

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A three-locus model for the chicken major histocompatibility complex

Cited by 206 publications

References 27 publications

Peptide motifs of the single dominantly expressed class I molecule explain the striking MHC-determined response to Rous sarcoma virus in chickens

Peptide motifs of the single dominantly expressed class I molecule explain the striking MHC-determined response to Rous sarcoma virus in chickens

Different Evolutionary Histories of the Two Classical Class I Genes BF1 and BF2 Illustrate Drift and Selection within the Stable MHC Haplotypes of Chickens

Histocompatible Chicken Inbred Lines: Homogeneities in the Major Histocompatibility Complex Antigens of the GSP, GSN/1, PNP/DO and BM-C Inbred Lines Assessed by Hemagglutination, Mixed Lymphocyte Reaction and Skin Transplantation

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