Histocompatible Chicken Inbred Lines: Homogeneities in the Major Histocompatibility Complex Antigens of the GSP, GSN/1, PNP/DO and BM-C Inbred Lines Assessed by Hemagglutination, Mixed Lymphocyte Reaction and Skin Transplantation

Valdez, Marcos B.; Mizutani, Makoto; Fujiwara, Akira; Yazawa, Hajime; Yamagata, T.; Shimada, Kiyoshi; Namikawa, Takao

doi:10.1538/expanim.56.329

Cited by 6 publications

(9 citation statements)

References 38 publications

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“…1). Thus, it is important to note that the typing of RBC antigens remains a major tool and marker in assessing the homogeniety of the chicken major histocompatibility complex (MHC) because it is encoded by a locus/loci that has close structural linkage related to the regions that control the MHC [13,17].…”

Section: Discussionmentioning

confidence: 99%

“…Antisera were prepared by a previously reported method using RBCs of a red jungle fowl against randomly selected chickens from the respective closed colonies [17]. Intact antisera were mixed with a 3% RBCs suspension washed with saline, and agglutination was determined after 60 min of incubation at room temperature.…”

Section: Preparation Of Antisera For Hemagglutination Testmentioning

confidence: 99%

“…The skin transplantation technique was based on a previous method [17]. Survival of the skingrafts was monitored for 3 weeks of post-transplantation to assess both the major (B system) and minor (C system) histocompatibility antigens of the different closed colonies of chicken.…”

Section: Skin Transplantation and Isohemagglutinin Formation Testmentioning

confidence: 99%

“…html) [17], we have maintained several chicken closed colonies for decades. The closed colonies of chicken used in this experiment have been studied for certain genetic characteristics such as mutations and blood-types (Table 1).…”

Section: Introductionmentioning

confidence: 99%

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Histocompatibility Assessment in the Chicken Colonies of the RIR-Y8/NU, YL, WL-G, and BL-E Closed for 28-48 Years

et al. 2009

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Histocompatibility was assessed in the RIR-Y8/NU, BL-E, YL, and WL-G chicken closed colonies by hemagglutination test using anti-red blood cell (RBC) antibodies (HT), skin transplantation test (STT), and formation of isohemagglutinins (FIHs) during STT. The YL individuals all showed the survival of skingrafts for more than 17 days with no FIHs in STT and no RBC antigenic variations in HT, indicating a histocompatible nature together with high homogeneity at serological loci. The BL-E as well as WL-G closed colonies were also found to be histocompatible in the STT with no FIHs, although the HT showed heterogeneities at serological locus/loci other than the B and C blood group loci which have significant effects on histocompatibility or FIHs in chicken. In the RIR-Y8/NU closed colonies, one individual in 6 reciprocal combinations of the STT showed early skingraft rejection with positive FIHs caused by different B locus alleles, and the HT suggested relatively high heterogeneities at the other serological loci too. The closed colonies of YL, BL-E, and WL-G will be useful avian materials for transplantation or related experiments, but RIR-Y8/NU needs further pedigree selection for serological homogeneity.

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Section: Discussionmentioning

confidence: 99%

Section: Preparation Of Antisera For Hemagglutination Testmentioning

confidence: 99%

Section: Skin Transplantation and Isohemagglutinin Formation Testmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Histocompatibility Assessment in the Chicken Colonies of the RIR-Y8/NU, YL, WL-G, and BL-E Closed for 28-48 Years

et al. 2009

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“…These lines are highly inbred and histocompatible [10]. Both lines were derived from the Fayoumi breed and differentiated on the basis of the red blood cell type antigen carried by each of the inbred lines (GSP -B 21 and PNP/DO -B 1 ) [10][11][12].…”

Section: Chicken Inbred Linesmentioning

confidence: 99%

Differential Development of Sex-related Characters of Chickens from the GSP and PNP/DO Inbred Lines after Left Ovariectomy

Valdez

Mizutani

Kinoshita

et al. 2010

Journal of Reproduction and Development

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Abstract. To elucidate strain differences in the sex reversal of genetic females to phenotypic males, GSP and PNP/DO females were left ovariectomized (ovx) between one to three days after hatching, and the degree of masculinization based on sex-related characters, histological analysis of the right gonad and hormone assay were assessed at one year of age. The GSP and PNP/DO inbred lines were both derived from the Fayoumi breed and are only differentiated based on the red blood cell antigen type carried by each inbred line. Combs and wattles were found to be significantly bigger (P<0.05) in the GSP ovx compared with the PNP/DO ovx chickens, although male plumage patterns were more pronounced in the PNP/DO ovx. Spurs were observed both in the GSP and PNP/DO ovx chickens with no significant difference (P>0.05) in length compared with the respective male controls, and body weight was not significantly different (P>0.05) compared with the female controls. The weight of the right gonad was significantly heavier (P<0.05) in the GSP ovx than in the PNP/DO ovx. Positive correlations were found in the sex-related characters as well as the plasma testosterone level and right gonad weight in both the GSP and PNP/DO ovx chickens, but not in the spur length, which showed a negative correlation in the PNP/DO ovx chickens. Histological analysis revealed that the right gonads of the PNP/DO ovx chickens were morphologically developed compared with the GSP ovx chickens, which showed more advance stages of spermatogenesis. It could be inferred that PNP/DO females that exhibit a hereditary persistent right oviduct are more responsive to the masculinizing effect of ovariectomy compared with GSP females, suggesting that genetic background may have a possible contribution to the degree of masculinization and subsequent development of sex related characters.

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Combined deletions of IHH and NHEJ1 cause chondrodystrophy and embryonic lethality in the Creeper chicken

et al. 2020

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The Creeper (Cp) chicken is characterized by chondrodystrophy in Cp/+ heterozygotes and embryonic lethality in Cp/Cp homozygotes. However, the genes underlying the phenotypes have not been fully known. Here, we show that a 25 kb deletion on chromosome 7, which contains the Indian hedgehog (IHH) and non-homologous end-joining factor 1 (NHEJ1) genes, is responsible for the Cp trait in Japanese bantam chickens. IHH is essential for chondrocyte maturation and is downregulated in the Cp/+ embryos and completely lost in the Cp/Cp embryos. This indicates that chondrodystrophy is caused by the loss of IHH and that chondrocyte maturation is delayed in Cp/+ heterozygotes. The Cp/Cp homozygotes exhibit impaired DNA double-strand break (DSB) repair due to the loss of NHEJ1, resulting in DSB accumulation in the vascular and nervous systems, which leads to apoptosis and early embryonic death.

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Histocompatible Chicken Inbred Lines: Homogeneities in the Major Histocompatibility Complex Antigens of the GSP, GSN/1, PNP/DO and BM-C Inbred Lines Assessed by Hemagglutination, Mixed Lymphocyte Reaction and Skin Transplantation

Abstract: Chicken inbred lines of the GSP, GSN/1, PNP/DO and BM-C have been established by selection of a specific allele at the B blood group locus (MHC

Cited by 6 publications

References 38 publications

Histocompatibility Assessment in the Chicken Colonies of the RIR-Y8/NU, YL, WL-G, and BL-E Closed for 28-48 Years

Histocompatibility Assessment in the Chicken Colonies of the RIR-Y8/NU, YL, WL-G, and BL-E Closed for 28-48 Years

Differential Development of Sex-related Characters of Chickens from the GSP and PNP/DO Inbred Lines after Left Ovariectomy

Combined deletions of IHH and NHEJ1 cause chondrodystrophy and embryonic lethality in the Creeper chicken

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