A Tightly Regulated Genetic Selection System with Signaling-Active Alleles of Phytochrome B

Hu, Wei; Lagarias, J. Clark

doi:10.1104/pp.16.01345

Cited by 7 publications

(11 citation statements)

References 63 publications

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“…The artificial light environment became one of the core technologies for tissue culture after De Capite explored the effects of light intensity on the growth of Helianthus annuus, Parthenocissus tricuspidata, and Daucus carota [16]. Light is pivotal for the growth and development of plants as the energy source for photochemical reactions in photosynthesis and as the chief trigger signal that regulates diverse biological effects on plants including photomorphogenesis [17,18], photosynthesis [19,20], physiological-biochemical characteristics [19,21], and development of tissues or organs [22,23]. A broad range of the light spectrum from 260 nm to 780 nm modulates plant growth and development throughout their lifecycle.…”

Section: Introductionmentioning

confidence: 99%

Light Quality Affects Growth and Physiology of Carpesium triste Maxim. Cultured In Vitro

et al. 2020

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The objective of this study was to investigate the effect of light quality on the growth and contents of photosynthetic pigments and total flavonoids, the parameters of chlorophyll a fluorescence, antioxidant capacities, and enzyme activities. It was intended to preliminarily explore the internal mechanisms involved for the effect of light quality on the growth and physiology of Carpesium triste Maxim. Stem apex explants were cultured on the Murashige and Skoog (MS) medium under white (W), red (R), blue (B), or a 1:1 mixture of red and blue (RB) light-emitting diodes (LEDs) for four weeks. The RB induced sturdy plantlets and increased the contents of photosynthetic pigments and total flavonoids, photosynthetic electron transport and efficiency, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging capacity, and activities of antioxidant enzymes in the plantlets. Taken together, combinations of monochromatic red and blue LEDs could be used for the best production of high-quality C. triste plantlets in vitro.

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Section: Introductionmentioning

confidence: 99%

Light Quality Affects Growth and Physiology of Carpesium triste Maxim. Cultured In Vitro

et al. 2020

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“…Upon light exposure, the second‐leaf blades of WT promptly expanded and greened, while those of Ubi::OsYHB mostly remained yellowish with possible greening a few days later (Figure 3f, Figure d). It was known that dark‐grown AtYHB Arabidopsis seedlings (>3‐day‐old) are photobleached and die upon light exposure, due to At YHB‐mediated suppression of protochlorophyllide reductase A ( PORA ) expression and activation of tetrapyrrole biosynthetic pathway that collectively results in phototoxicity of the dark‐accumulated protochlorophyllide (Hu & Lagarias, 2017). For OsYHB rice plants, however, the third leaves green normally after light exposure.…”

Section: Resultsmentioning

confidence: 99%

“…For OsYHB rice plants, however, the third leaves green normally after light exposure. Such de‐etiolation differences likely arise from the mild twofold downregulation of OsPORA by OsYHB or red light in rice (Table , see below) compared with the ~30‐fold downregulation of AtPORA by AtYHB in dark‐grown Arabidopsis (Hu & Lagarias, 2017; Hu et al, 2009).…”

Section: Resultsmentioning

confidence: 99%

Regulation of monocot and dicot plant development with constitutively active alleles of phytochrome B

Figueroa‐Balderas

Chi-Ham

et al. 2020

Plant Direct

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This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. AbstractThe constitutively active missense allele of Arabidopsis phytochrome B, AtPHYB Y276H or AtYHB, encodes a polypeptide that adopts a light-insensitive, physiologically active conformation capable of sustaining photomorphogenesis in darkness. Here, we show that the orthologous OsYHB allele of rice phytochrome B (OsPHYB Y283H ) also encodes a dominant "constitutively active" photoreceptor through comparative phenotypic analyses of AtYHB and OsYHB transgenic lines of four eudicot species, Arabidopsis thaliana, Nicotiana tabacum (tobacco), Nicotiana sylvestris and Solanum lycopersicum cv. MicroTom (tomato), and of two monocot species, Oryza sativa ssp. japonica and Brachypodium distachyon. Reciprocal transformation experiments show that the gainof-function constitutive photomorphogenic (cop) phenotypes by YHB expression are stronger in host plants within the same class than across classes. Our studies also reveal additional YHB-dependent traits in adult plants, which include extreme shade tolerance, both early and late flowering behaviors, delayed leaf senescence, reduced tillering, and even viviparous seed germination. However, the strength of these gainof-function phenotypes depends on the specific combination of YHB allele and species/cultivar transformed. Flowering and tillering of OsYHB-and OsPHYB-expressing lines of rice Nipponbare and Kitaake cultivars were compared, also revealing differences in YHB/PHYB allele versus genotype interaction on the phenotypic behavior of the two rice cultivars. In view of recent evidence that the regulatory activity of AtYHB is not only light insensitive but also temperature insensitive, selective YHB expression is expected to yield improved agronomic performance of both dicot and monocot crop plant species not possible with wild-type PHYB alleles.

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“…Protein extraction and immunoblot assays were performed as previously described (Su and Lagarias 2007 ; Jones et al 2015 ; Hu and Lagarias 2017 ). Blot intensities were quantified using LI-COR Image StudioLite software (for phyB and tubulin) or ImageJ (for PIF3).…”

Section: Methodsmentioning

confidence: 99%

A cytosol-tethered YHB variant of phytochrome B retains photomorphogenic signaling activity

Hu,

Lagarias

2024

Plant Mol Biol

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The red and far-red light photoreceptor phytochrome B (phyB) transmits light signals following cytosol-to-nuclear translocation to regulate transcriptional networks therein. This necessitates changes in protein–protein interactions of phyB in the cytosol, about which little is presently known. Via introduction of a nucleus-excluding G767R mutation into the dominant, constitutively active phyBY276H (YHB) allele, we explore the functional consequences of expressing a cytosol-localized YHBG767R variant in transgenic Arabidopsis seedlings. We show that YHBG767R elicits selective constitutive photomorphogenic phenotypes in dark-grown phyABCDE null mutants, wild type and other phy-deficient genotypes. These responses include light-independent apical hook opening, cotyledon unfolding, seed germination and agravitropic hypocotyl growth with minimal suppression of hypocotyl elongation. Such phenotypes correlate with reduced PIF3 levels, which implicates cytosolic targeting of PIF3 turnover or PIF3 translational inhibition by YHBG767R. However, as expected for a cytoplasm-tethered phyB, YHBG767R elicits reduced light-mediated signaling activity compared with similarly expressed wild-type phyB in phyABCDE mutant backgrounds. YHBG767R also interferes with wild-type phyB light signaling, presumably by formation of cytosol-retained and/or otherwise inactivated heterodimers. Our results suggest that cytosolic interactions with PIFs play an important role in phyB signaling even under physiological conditions.

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A Tightly Regulated Genetic Selection System with Signaling-Active Alleles of Phytochrome B

Cited by 7 publications

References 63 publications

Light Quality Affects Growth and Physiology of Carpesium triste Maxim. Cultured In Vitro

Light Quality Affects Growth and Physiology of Carpesium triste Maxim. Cultured In Vitro

Regulation of monocot and dicot plant development with constitutively active alleles of phytochrome B

A cytosol-tethered YHB variant of phytochrome B retains photomorphogenic signaling activity

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