A ‘tissue model’ to study the barrier effects of living tissues on the reactive species generated by surface air discharge

et al. 2018

Self Cite

The transportation of reactive oxygen species (ROS) to lesion site through human tissue is crucial for plasma medicine. For this reason, the spatial‐temporal distributions of ROS in model tissues irradiated by a He + O2 plasma jet were investigated in this paper. It was found that the ROS formed a ring‐shaped surface pattern on the model tissue, of which the diameter increased with the gas flow rate but remained unchanged with the plasma treatment time. The surface pattern changed significantly with the inclination angle of plasma irradiation, suggesting that the ROS dosage is difficult to precisely control for clinical applications. Moreover, the penetration depth of O3 increased linearly with the plasma treatment time or the water content of the model tissue.

Section: Resultssupporting

confidence: 90%

Section: Experimental Setup and Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 92%

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Spatial‐temporal distributions of ROS in model tissues treated by a He+O₂ plasma jet

et al. 2018

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“…Here, we replaced the diffusivity of species in the 3D tumor spheroids with the values in water. In fact, the diffusivity of species in tissues was lower than that in water, which may result in a higher penetration depth. The initial concentrations of H 2 O 2 , NO 2 − , NO 3 − , O 2 − , and OH• on the surface of the 3D tumor spheroids were replaced by the average concentrations in the cell‐culture solution (Figure ).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the anticancer effects induced by cold atmospheric plasma in 2D and 3D cell‐culture models

Zhang

et al. 2019

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The biological characteristics of three‐dimensional (3D) tumors cannot be adequately represented through two‐dimensional (2D) cell cultures. Here, to acquire more precise information about plasma–tumor interactions, the plasma‐induced cytotoxicity was tested in both 2D cell culture and 3D tumor‐spheroid culture. The results indicated that the toxic effects were markedly reduced in 3D cancer cells compared with 2D cells. We found that reactive oxygen and nitrogen species can penetrate through the entire depth of 3D tumor spheroid, but local apoptosis was observed only on the outermost layer. The permeation depth of plasma‐generated reactive species was then tracked in 3D tumor spheroids. We hypothesized that plasma‐induced apoptosis within a tumor depends on the “deep” delivery of H2O2, NO2−, and NO3− rather than O2−, OH•, and ONOOH/ONOO−.

“…(f) Confocal microscopy images of the synthetic biological sensor after perpendicular CAP jet treatment on gelatin matrix for 300 s. [ 129 ] (g) The small electric fields enhanced the permeability of RONS in gelatin gel. [ 130 ] (h) Agarose gel after CAP treatment with H 2 O 2 fluorescent dye. [ 131 ] (i) The CAP jet propagated inside of a T‐shaped agarose tube.…”

Section: Transport Of Reactive Agentsmentioning

confidence: 99%

Cold atmospheric pressure plasmas in dermatology: Sources, reactive agents, and therapeutic effects

Zhang

et al. 2020

Recently, cold atmospheric pressure plasmas (CAPs) have provided many new opportunities in dermatology by providing a multimodal action of reactive agents (RA). This review critically examines the generation, transport, and physical effects induced by CAPs in dermatologic treatments. We introduce the most suitable plasma sources, which provide a multitude of physical effects on the skin without any electric or thermal shocks. The mechanisms of generation and transport of the key reactive species are introduced and examined from the viewpoint of their applications in dermatology. Special attention is paid to study the “plasmaporation” effect, which enables RA penetration through healthy and damaged skin. Plausible physical mechanisms involved in the CAP treatment of some of the most common skin diseases are analyzed.