A transcriptional journey from sucrose to endosperm oil bodies in triple transgene oily wheat grain

Larkin, P. J.; Zhou, Xue-Rong; Liu, Q.; Reynolds, Kyle B.; Vanhercke, Thomas; Ral, Jean‐Philippe; Li, Z.; Wu, Xi; Yu, Ronald; Luo, Jixun; Newberry, Marcus; Howitt, Crispin A.

doi:10.1016/j.jcs.2021.103268

Cited by 6 publications

(4 citation statements)

References 46 publications

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“…Our previous analyses of AsWRI1 -wheat endosperms showed, in addition to increased levels of TAG and transcripts encoding functions in fatty acid synthesis and oleosins, increased levels of free fatty acids throughout grain development and transcripts coding for genes involved in the degradation of lipids and fatty acids [ 24 ]. This is in contrast to in the ‘push-pull-protect oil-wheat’ in which none of the genes encoding functions in lipid degradation were up-regulated [ 40 ]. Altogether, this indicates that the expression of the transcription factor WRI1 alone causes a carbon metabolism imbalance in the wheat endosperm such that the starch synthesis is reduced, but the TAG synthesis machinery is not able to utilize all available fatty acids from de novo synthesis.…”

Section: Discussionmentioning

confidence: 69%

“…In a recent study, a ‘push-pull-protect’ approach was used in wheat, by combining endosperm-specific expression of WRI1 with genes coding for an acyltransferase involved in TAG assembly and an oleosin important for proper packaging of oil. This resulted in a significantly increased oil content in the grains while the starch content and seed weight were retained [ 39 , 40 ]. A comparison between these previous results with ours allows for an increased understanding of how the metabolism of the wheat endosperm is affected by different biotechnological approaches to develop wheat grains with increased oil content.…”

Section: Discussionmentioning

confidence: 99%

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Characterisation of Grains and Flour Fractions from Field Grown Transgenic Oil-Accumulating Wheat Expressing Oat WRI1

Snell

Wilkinson

Taylor

et al. 2022

Plants

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Wheat (Triticum aestivum L.) is one of the major staple crops in the world and is used to prepare a range of foods. The development of new varieties with wider variation in grain composition could broaden their use. We characterized grains and flours from oil-accumulating transgenic wheat expressing the oat (Avena sativa L.) endosperm WRINKLED1 (AsWRI1) grown under field conditions. Lipid and starch accumulation was determined in developing caryopses of AsWRI1-wheat and X-ray microtomography was used to study grain morphology. The developing caryopses of AsWRI1-wheat grains had increased triacylglycerol content and decreased starch content compared to the control. Mature AsWRI1-wheat grains also had reduced weight, were wrinkled and had a shrunken endosperm and X-ray tomography revealed that the proportion of endosperm was decreased while that of the aleurone was increased. Grains were milled to produce two white flours and one bran fraction. Mineral and lipid analyses showed that the flour fractions from the AsWRI1-wheat were contaminated with bran, due to the effects of the changed morphology on milling. This study gives a detailed analysis of grains from field grown transgenic wheat that expresses a gene that plays a central regulatory role in carbon allocation and significantly affects grain composition.

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Section: Discussionmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Characterisation of Grains and Flour Fractions from Field Grown Transgenic Oil-Accumulating Wheat Expressing Oat WRI1

Snell

Wilkinson

Taylor

et al. 2022

Plants

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“…Soluble sugar components undergo a significant changing period in the process of oil crop seed development, which will directly or indirectly affect the oil accumulation of late-stage seeds [40]. The expression of the sucrose synthase gene is higher in the middle and later stages of seed development, which may indicate the transformation of sugars to oil [41]. According to the correlation analysis results of the current study, the oil content of grain was negatively correlated with SS and SUC but positively correlated with ST, indicating that the decrease in SS and SUC contents is likely because they are the main carbon source of oil synthesis.…”

Section: Discussionmentioning

confidence: 99%

Effect of Different Types of Organic Manure on Oil and Fatty Acid Accumulation and Desaturase Gene Expression of Oilseed Flax in the Dry Areas of the Loess Plateau of China

Ma,

Gao,

et al. 2024

Agronomy

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In order to understand the mechanism of action of oil and fatty acid accumulation and desaturase gene expression in how oilseed flax responds to different fertilization conditions, a three-factor split-plot experiment was conducted to investigate the accumulation trends of oil and fatty acids. The results revealed that soluble sugar (SS) and sucrose (SUC) contents decreased, and the starch (ST) content increased gradually with the grain development and maturity of oilseed flax. The application of sheep manure promoted the accumulation of nonstructural carbohydrates in the grains. Soluble sugar (SS) and sucrose (SUC) contents were negatively correlated with the oil content. Compared with chemical fertilizer, organic manure decreased the total saturated fatty acid but increased the unsaturated fatty acid. Organic manure significantly upregulated the expression of various genes, and fad2a gene expression was higher with the 5.8 t ha−1 chicken manure treatment. The 25 t ha−1 sheep manure treatment was more conducive to the expression of fad3a and fad3b genes and promoted the accumulation of linolenic acid (LIN), and the LIN content increased by 0.64–3.90% compared with other treatments. Consequently, an ongoing anthropogenic sheep manure input may impact the regulation of grain oil quality and yield per unit area.

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“…Anoxia-induced plant cell ATP levels decreased to reduce the activity of long-chain ACSL, leading to change in the acyl-CoA pool [ 87 ]. Transcriptomic analysis at different developmental stages indicated that upregulation of LACS8 promoted the transportation of fatty acid precursors to the endoplasmic reticulum in wheat [ 88 ]. Our results allowed us to assume that chilling-induced mobile Cmo mRNA of ACSL (CmoCh04G007350) may participate in accumulation of the Csa acyl-CoA pool, thereby regulating subsequent fatty acid elongation metabolism.…”

Section: Discussionmentioning

confidence: 99%

Rootstock–scion exchanging mRNAs participate in the pathways of amino acid and fatty acid metabolism in cucumber under early chilling stress

Liu

Wang

Zhang

et al. 2022

Horticulture Research

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Cucumber (Cucumis sativus L.) often experiences chilling stress that limits their growth and productivity. Grafting is widely used to improve abiotic stress resistance by alternating a vigorous root system, suggesting there exists systemic signals communication between distant organs. mRNAs are reported to be evolving in fortification strategies by long-distance signaling when plants suffering from chilling stress. However, the potential function of mobile mRNAs alleviating chilling stress in grafted cucumber is still unknown. Here, the physiological changes, mobile mRNAs profiling, transcriptomic and metabolomic changes in above- and underground tissues of all graft combinations of cucumber and pumpkin responding to chilling stress were established and analyzed comprehensively. The co-relationship between the cluster of chilling-induced pumpkin mobile mRNAs with Differentially Expressed Genes (DEGs) and Differentially Intensive Metabolites (DIMs) revealed that four key chilling-induced pumpkin mobile mRNAs were highly related to glycine, serine and threonine synthesis and fatty acid β-oxidative degradation metabolism in cucumber tissues of heterografts. The verification of mobile mRNAs, potential transport of metabolites and exogenous application of key metabolites of glycerophospholipid metabolism pathway in cucumber seedlings confirmed that the role of mobile mRNAs in regulating chilling responses in grafted cucumber. Our results build a link between the long-distance mRNAs of chilling-tolerant pumpkin and the fatty acid β-oxidative degradation metabolism of chilling-sensitive cucumber. It helps to uncover the mechanism of signaling interaction between scion and stock responding to chilling tolerant in grafted cucumber.

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A transcriptional journey from sucrose to endosperm oil bodies in triple transgene oily wheat grain

Cited by 6 publications

References 46 publications

Characterisation of Grains and Flour Fractions from Field Grown Transgenic Oil-Accumulating Wheat Expressing Oat WRI1

Characterisation of Grains and Flour Fractions from Field Grown Transgenic Oil-Accumulating Wheat Expressing Oat WRI1

Effect of Different Types of Organic Manure on Oil and Fatty Acid Accumulation and Desaturase Gene Expression of Oilseed Flax in the Dry Areas of the Loess Plateau of China

Rootstock–scion exchanging mRNAs participate in the pathways of amino acid and fatty acid metabolism in cucumber under early chilling stress

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