A Triple Stain for Deoxyribonucleic Acid, Polysaccharides and Proteins

Structure and function of vulture pecten

“…Cornoy-fixed material was stained with methyl green-pyronin [Kt bnick, 1952] Mowry, 1958] for glycogen with controls run simultaneously after malt-diastase (1 % malt-diastase in 0.2 M phosphate buffer of pH 7.0) treatment for 4 h at 40 °C. Triple stain [Himes and Moriber, 1956] was employed on Zenker-fixed tissue sec tions for combined staining of carbohydrates, proteins and DNA. Thionin [Clarke and Sperry, 1945] was employed on Bouin-fixed sections for the study of Nissl substance.…”

The Contribution of the Bacterial Components of Adjuvant in the Induction of Aspermatogenesis and in the Sensitization of the Ilea of Guinea Pigs

“…Portions of the testes, seminal vesicle, vas deferens, epididymis, and ileum were placed in fixative, sectioned, and stained according to Heidenhain's azan method, as well as with a triple stain (Himes and Moriber, 1956) and for mast cells (Smith and Atkinson, 1956). When samples of the testicular tissues were examin ed histologically, the degree of spermatogenic tissue destruction was rated according to a scale previously described (Katsh and Bishop, 1958) which employed ascending numerals from 0 to 4 to indicate a range of effect from none to complete elimination of gametogenic elements.…”

Identification of the Mouse Submandibular Gland Cells in Situ and of Isolated Single Cells With a Triple Staining Method

“…With the present staining procedure, in contrast to the observations by Himes and Moriber (1956) with azur A-and basic fuchsin-Schiff reagents, the two Schiff-reagents could not be used in the reversed sequence. In our study, the cells stained with Feulgen reaction using pararosanilin-Schiff reagent remained unstained with the subsequent PAS reaction using azur B-Schiff reagent, whereas the nuclei stained first with Feulgen reaction using azur B-Schiff reagent were partially restained with PAS reaction using pararosanilin-Schiff reagent.…”