A Trypsin Inhibitor from Rambutan Seeds with Antitumor, Anti-HIV-1 Reverse Transcriptase, and Nitric Oxide-Inducing Properties

Fang, Evandro Fei; Ng, Tzi Bun

doi:10.1007/s12010-015-1550-1

Cited by 27 publications

(12 citation statements)

References 36 publications

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“…For instance, Fang et al [68] isolated an inhibitor from Korean large black soybeans (KBTI), which exerted a weak antiproliferative activity toward CNE-2 and HNE-2 nasopharyngeal cancer cells, MCF-7 breast cancer cells, and Hep G2 hepatoma cells. Moreover, Fang and Ng [69] isolated a trypsin inhibitor from Nephelium lappaceum L. seeds (NLTI) which inhibited many tumor cells. However, the trypsin inhibitor from Enterolobium contortisiliquum seeds (EcTI) showed no effect on the proliferation of gastric cancer cells but inhibited the adhesion, migration, and cell invasion of these tumor cells [70].…”

Section: Discussionmentioning

confidence: 99%

Exploiting the biological roles of the trypsin inhibitor from Inga vera seeds: A multifunctional Kunitz inhibitor

Bezerra

Oliveira

Machado

et al. 2016

Process Biochemistry

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Section: Discussionmentioning

confidence: 99%

Exploiting the biological roles of the trypsin inhibitor from Inga vera seeds: A multifunctional Kunitz inhibitor

Bezerra

Oliveira

Machado

et al. 2016

Process Biochemistry

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“…Trypsin inhibitors (TIs) are widely distributed in plants and animals, and certain types of TIs have exhibited antitumor activity in several types of tumor cells (3)(4)(5). Although no approved therapies directly targeting trypsin directly are currently available, development of novel and more specific TI therapies is underway (3)(4)(5).…”

Section: Introductionmentioning

confidence: 99%

“…Although no approved therapies directly targeting trypsin directly are currently available, development of novel and more specific TI therapies is underway (3)(4)(5). Sporamin, a sweet potato tuber storage protein, is a Kunitz-type TI that has been demonstrated to induce antitumor effects in vitro and in vivo (4,5).…”

Section: Introductionmentioning

confidence: 99%

Mitogen‑activated protein kinase inhibition enhances the antitumor effects of sporamin in human pancreatic cancer cells

Qian

Zhong

et al. 2018

Oncol Lett

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Abstract. Sporamin, a sweet potato tuber storage protein, is a Kunitz-type trypsin inhibitor (TI) that has exhibited antitumor activity through poorly defined mechanisms in a number of types of tumor cells. The present study aimed to analyze the combined effects of sporamin and three mitogen-activated protein kinase (MAPK) inhibitors, PD98059, SP600125 and SB203580, on the pancreatic cancer cell line, PANC-1. Cell proliferation activity was assessed using a 3 H-thymidine incorporation assay, and cell viability was analyzed using an MTT assay. Apoptosis was assayed by flow cytometry and fluorescence microscopy. Protein expression levels in PANC-1 cells were determined by western blotting. The results of this analysis demonstrated that sporamin induced a temporary increase in the phosphorylation of MAPKs, including phosphorylated extracellular signal regulated-kinase 1/2, phosphorylated c-Jun amino-terminal protein kinase 1/2 and phosphorylated p38-MAPK, in a concentration-dependent manner. However, treatment with MAPK inhibitors promoted the inhibition of cell proliferation and viability, and the induction of apoptosis in sporamin-treated PANC-1 cells.In conclusion, the present study demonstrated that MAPK inhibition enhanced the antitumor activity of sporamin in PANC-1 cells. IntroductionPancreatic cancer (PC) is among the most aggressive types of solid tumor, and has the highest rate of cancer-associated mortality worldwide (1). Despite advances in surgical techniques, chemotherapy and radiotherapy, the long-term survival rate for patients with PC has not significantly increased since 1977 (2). Therefore, further research into alternative therapies for PC, which has fewer associated toxicities, is urgently required.Trypsin inhibitors (TIs) are widely distributed in plants and animals, and certain types of TIs have exhibited antitumor activity in several types of tumor cells (3-5). Although no approved therapies directly targeting trypsin directly are currently available, development of novel and more specific TI therapies is underway (3-5). Sporamin, a sweet potato tuber storage protein, is a Kunitz-type TI that has been demonstrated to induce antitumor effects in vitro and in vivo (4,5). Although the role and mechanism of sporamin as an antitumor agent remain unclear, the previous research has indicated that sporamin can mediate the regulation of specific signaling pathways (5). For example, sporamin was demonstrated to inhibit cell growth and induce apoptosis of human tongue cancer cells by downregulating RAC serine/threonine-protein kinase/glycogen synthase kinase-3 signaling (5). However, to the best of our knowledge, the antitumor effects of sporamin in PC have not been previously investigated.Mitogen-activated protein kinases (MAPK) are critically involved in the signaling cascades that govern various critical processes, including cell proliferation, differentiation, apoptosis and survival (6). MAPKs are conserved enzymes that connect cell-surface receptors to their regulatory targets within cells (7). Th...

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“…3. Inhibiting proliferation via the activation of caspase-3 and caspase-9, up-regulation of the ratio of bax/bcl-2 protein expression in Ardisia brevicaulis [61] Artocarpus obtusus [50,62] Ardisia brecaulis [63], Carica papaya [64] Catharanthus roseus [118][119], Costus speciosus [121][122], Cucuma zedoaria [65], Dysoxylum cauliforum [66], Goniosthalamus macrophyllus [137][138], Gynura procumbens [139], Lawsonia inermis [56,[146][147], Leea indica [148][149], Nepthelium lappaceum [56,156] Pandanus amarylfolius [41], Phyla nodiflora [67], Physalis minima [52,78], Rhodiola rosea [68], Vernonia amygdalina [65] and Schima wallichi [38]. 4.…”

Section: Resultsmentioning

confidence: 99%

Malaysian Medicinal Plants’ Potential for Breast Cancer Therapy

Mainasara

Bakar

Linatoc

2018

Asian J Pharm Clin Res

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Objective: This review focused on Malaysian medicinal plants that have been evaluated and pose potentials to treat breast cancer. Results: A total of 105 plants species representing 54 different families and 79 genera were reviewed. 97% of the plants were tested using MCF-7 and MDA-231 breast cancer cell lines and exhibited most significant in vitro anticancer activity, and 3% were tested using another type of breast cancer cell lines. Most of the bioactive compounds of the medicinal plants that exhibited good activity (IC 50 values <120 µg/mL) are a group of phenols, alkaloids, flavonoids, terpenoids, and saponins. Induction of apoptosis was found to be the significant cell death pathway. Methods Conclusion:This article reviews the available literature concerning research on anti-breast cancer plants. Furthermore, identification and characterization of active components and toxicology evaluation also need to be studied in details and also point out their clinical trials.

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A Trypsin Inhibitor from Rambutan Seeds with Antitumor, Anti-HIV-1 Reverse Transcriptase, and Nitric Oxide-Inducing Properties

Cited by 27 publications

References 36 publications

Exploiting the biological roles of the trypsin inhibitor from Inga vera seeds: A multifunctional Kunitz inhibitor

Exploiting the biological roles of the trypsin inhibitor from Inga vera seeds: A multifunctional Kunitz inhibitor

Mitogen‑activated protein kinase inhibition enhances the antitumor effects of sporamin in human pancreatic cancer cells

Malaysian Medicinal Plants’ Potential for Breast Cancer Therapy

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