A two-center international evaluation of the Immulite 2000 automated serum gastrin assay

Eastvold, Mary L.; Wallinder, H; Preissner, Carol M.; Singh, Ravinder J.; Löwbeer, Christian; Grebe, Stefan K.

doi:10.1016/j.clinbiochem.2006.01.004

Cited by 4 publications

(4 citation statements)

References 4 publications

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“… AUROC area under the ROC curve, CE chronic enteropathy, H2A histamine type-2 receptor antagonist, PPI proton pump inhibitor, URL upper reference limit a historical positive controls [ 4 , 21 – 30 ] …”

Section: Resultsmentioning

confidence: 99%

“…Serum gastrin concentration was measured following routine laboratory procedures (i.e., within 24 h after samples were received at the laboratory) using an automated commercially available chemiluminometric assay 4 , 5 [ 21 ]. The lower limit of detection of the assay is 9.9 ng/L, and the intra- and inter-assay variability of the assay are <8% [ 21 ]. The upper reference limit (URL) of the assay has been previously established as ≤27.8 ng/L using serum samples from 41 healthy dogs 5 .…”

Section: Methodsmentioning

confidence: 99%

“…Intra- and inter-individual biological variation of gastrin concentrations in serum were assessed using serial samples from 45 dogs with CE (Fig. 1 ), with the analytical variation (CV A ) set at 8% based on previous findings [ 21 ]. Following tests for outliers at 2 levels (i.e., intra- and inter-individual variation), a nested ANOVA model served to calculate the biologic coefficients of variation (CV): intra-individual (CV I ), inter-individual (CV G ), and total variation (CV T ) [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

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Effect of gastric acid-suppressive therapy and biological variation of serum gastrin concentrations in dogs with chronic enteropathies

et al. 2017

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BackgroundSerum gastrin concentration can help diagnose gastrinomas in dogs if >3–10× the upper reference limit (URL), but antisecretory therapy and other conditions can also cause hypergastrinemia. Effects of antisecretory therapy (famotidine or ranitidine, omeprazole) on serum gastrin concentration in dogs with chronic enteropathy (CE) and its biological variation (BV) are unknown. Aim of the study was to evaluate serum gastrin in acid-suppressant-treated or -naïve CE dogs; test the association between serum gastrin and histopathologic findings in acid-suppressant-naïve CE dogs; and evaluate the BV of serum gastrin in dogs not receiving any gastric acid suppressive therapy. Samples from 231 dogs were used and serum gastrin was measured by chemiluminescence assay. Gastric and duodenal histologic lesions were evaluated and graded. BV of serum gastrin was evaluated in serial samples.ResultsSerum gastrin concentrations were significantly higher in acid-suppressant-treated than acid-suppressant-naïve dogs (P = 0.0245), with significantly higher concentrations in proton pump inhibitor (PPI)- than H2-antihistamine-treated patients (P = 0.0053). More PPI- than H2-antihistamine-treated dogs had gastrin concentrations above URL (P = 0.0205), but not >3× nor >10× the URL. Serum gastrin concentrations correlated with the severity of gastric antral epithelial injury (P = 0.0069) but not with any other lesions or the presence/numbers of spiral bacteria in gastric biopsies. Intra- and inter-individual BV were 43.4 and 21.6%, respectively, in acid-suppressant-naïve dogs, with a reciprocal individuality index of 0.49 and a critical difference of ≥29.5 ng/L.ConclusionsAntisecretory (particularly PPI) treatment leads to hypergastrinemia in CE dogs, but the concentrations seen in this study are unlikely to compromise a diagnosis of gastrinoma. Use of a population-based URL for canine serum gastrin and a URL of ≤27.8 ng/L are appropriate.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Effect of gastric acid-suppressive therapy and biological variation of serum gastrin concentrations in dogs with chronic enteropathies

et al. 2017

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“…Based on other studies, accuracy or spiking recovery of the cobalamin immunoassay was determined by calculating O/E ratios for 5 different porcine serum samples that had been spiked with each other in a 1:1 dilution. Spiking recovery for the folate immunoassay was evaluated by using 5 different porcine serum samples that had been spiked with each other in a 1:3 dilution so that the concentrations were in the assay working range.…”

Section: Methodsmentioning

confidence: 99%

Analytic validation of commercially available immunoassays for the measurement of serum cobalamin and folate concentrations in pigs

Grützner

Knabe

Lawhorn

et al. 2016

Veterinary Clinical Pathol

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Estabilidad de las concentraciones de factor de crecimiento similar a la insulina tipo 1, gastrina y androstendiona en suero

Castillo

Osaba

Macias

et al. 2009

Revista del Laboratorio Clínico

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A two-center international evaluation of the Immulite 2000 automated serum gastrin assay

Cited by 4 publications

References 4 publications

Effect of gastric acid-suppressive therapy and biological variation of serum gastrin concentrations in dogs with chronic enteropathies

Effect of gastric acid-suppressive therapy and biological variation of serum gastrin concentrations in dogs with chronic enteropathies

Analytic validation of commercially available immunoassays for the measurement of serum cobalamin and folate concentrations in pigs

Estabilidad de las concentraciones de factor de crecimiento similar a la insulina tipo 1, gastrina y androstendiona en suero

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