A Two‐Channel Ratiometric Electrochemical Biosensor for In Vivo Monitoring of Copper Ions in a Rat Brain Using Gold Truncated Octahedral Microcages

Chai, Xiaolan; Zhou, Xin; Zhu, Anwei; Zhang, Limin; Qin, Yao; Shi, Guoyue; Tian, Yang

doi:10.1002/ange.201302958

Cited by 25 publications

(5 citation statements)

References 39 publications

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“…Au nanocages were obtained according to the recently reported 30 with some modication. To prepare the Au nanocages, Cu 2 O cube precursors were rst synthesized.…”

Section: Preparation Of the Au Nanocages And Ru(bpy)mentioning

confidence: 99%

A disposable paper-based electrochemiluminescence device for ultrasensitive monitoring of CEA based on Ru(bpy)₃²⁺@Au nanocages

Gao

Wang

et al. 2015

RSC Adv.

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“…Au nanocages were obtained according to the recently reported 30 with some modication. To prepare the Au nanocages, Cu 2 O cube precursors were rst synthesized.…”

Section: Preparation Of the Au Nanocages And Ru(bpy)mentioning

confidence: 99%

A disposable paper-based electrochemiluminescence device for ultrasensitive monitoring of CEA based on Ru(bpy)₃²⁺@Au nanocages

Gao

Wang

et al. 2015

RSC Adv.

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“…Ratiometric detection has extensively been developed in fluorescence and electrochemiluminescence analysis of biomolecules 42 43 44 . Recently this technology has been employed in voltammetric detection of metal ion using a two-channel biosensor array 45 . Similar to a recently reported sensing interface, which was structured using a methylene blue (MB)-labeled DNA modified gold electrode to hybridize ferrocene (Fc)-labeled aptamer probe for detection of ATP with the sum of peak current changes 46 , this work immobilized Fc-labeled DNA probe (Fc-P) and MB-labeled antibody-DNA (MB-DNA1-Ab1) at the same electrode.…”

mentioning

confidence: 99%

Ratiometric electrochemical proximity assay for sensitive one-step protein detection

Ren

Yan

et al. 2014

Sci Rep

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This work proposes the concept of ratiometric electrochemical proximity assay (REPA), which can be used for one-step, highly sensitive and selective detection of protein. The assay strategy was achieved on a sensing interface that was formed by hybridization of methylene blue (MB)-labeled antibody-DNA probe (MB-DNA1-Ab1) with ferrocene (Fc)-labeled DNA capture probe (Fc-P) modified gold electrode. On the interface the target protein could trigger the formation of immunocomplex between MB-DNA1-Ab1 and detection antibody-DNA probe (Ab2-DNA2) and subsequently the proximity hybridization of DNA1-DNA2, which led to the departure of MB-DNA1-Ab1 from the interface. The remained Fc-P could form a hairpin structure to take Fc group to electrode surface. Therefore, the recognition of target protein to Ab1 and Ab2 resulted in both the “signal-off” of MB and the “signal-on” of Fc for dual-signal electrochemical ratiometric readout. The proposed REPA could be carried out in one-step with 40-min duration and showed a wide detection range from 0.05 to 100 ng/mL with pg/mL limit of detection, displaying great potential for convenient point-of-care testing and commercial application.

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“…The dual-channel ratiometric electrochemical biosensor initially adopts two independent working electrodes, which are modified with specific recognition elements, respectively. 140,141 To reduce brain injury during operation and inaccuracy between two electrodes, recognition and reference molecules are integrated onto a single electrode, realizing in vivo qualitative measurement of ions such as H +142 and Cu 2+143 (Figure 3). Different from the ratiometric strategy that offers insights into design principles, ISE as one of the most welldeveloped potentiometric sensors is experiencing its rebirth in the field of in vivo electrochemical sensing.…”

Section: ■ Selectivitymentioning

confidence: 99%

In Vivo Electrochemical Sensors for Neurochemicals: Recent Update

2019

ACS Sens.

122

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In vivo electrochemical sensing based on implantable microelectrodes is a strong driving force of analytical neurochemistry in brain. The complex and dynamic neurochemical network sets stringent standards of in vivo electrochemical sensors including high spatiotemporal resolution, selectivity, sensitivity, and minimized disturbance on brain function. Although advanced materials and novel technologies have promoted the development of in vivo electrochemical sensors drastically, gaps with the goals still exist. This Review mainly focuses on recent attempts on the key issues of in vivo electrochemical sensors including selectivity, tissue response and sensing reliability, and compatibility with electrophysiological techniques. In vivo electrochemical methods with bare carbon fiber electrodes, of which the selectivity is achieved either with electrochemical techniques such as fast-scan cyclic voltammetry and differential pulse voltammetry or based on the physiological nature will not be reviewed. Following the elaboration of each issue involved in in vivo electrochemical sensors, possible solutions supported by the latest methodological progress will be discussed, aiming to provide inspiring and practical instructions for future research.

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A Two‐Channel Ratiometric Electrochemical Biosensor for In Vivo Monitoring of Copper Ions in a Rat Brain Using Gold Truncated Octahedral Microcages

Cited by 25 publications

References 39 publications

A disposable paper-based electrochemiluminescence device for ultrasensitive monitoring of CEA based on Ru(bpy)₃²⁺@Au nanocages

A disposable paper-based electrochemiluminescence device for ultrasensitive monitoring of CEA based on Ru(bpy)₃²⁺@Au nanocages

Ratiometric electrochemical proximity assay for sensitive one-step protein detection

In Vivo Electrochemical Sensors for Neurochemicals: Recent Update

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A Two‐Channel Ratiometric Electrochemical Biosensor for In Vivo Monitoring of Copper Ions in a Rat Brain Using Gold Truncated Octahedral Microcages

Cited by 25 publications

References 39 publications

A disposable paper-based electrochemiluminescence device for ultrasensitive monitoring of CEA based on Ru(bpy)32+@Au nanocages

A disposable paper-based electrochemiluminescence device for ultrasensitive monitoring of CEA based on Ru(bpy)32+@Au nanocages

Ratiometric electrochemical proximity assay for sensitive one-step protein detection

In Vivo Electrochemical Sensors for Neurochemicals: Recent Update

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A disposable paper-based electrochemiluminescence device for ultrasensitive monitoring of CEA based on Ru(bpy)₃²⁺@Au nanocages

A disposable paper-based electrochemiluminescence device for ultrasensitive monitoring of CEA based on Ru(bpy)₃²⁺@Au nanocages