A two-ply artificial blood vessel of polyurethane and poly(L-lactide)

Pennings, A. J.; Knol, Ke; Hoppen, Hj; Leenslag, Jw; Vanderlei, B

doi:10.1007/bf01410416

Cited by 28 publications

(18 citation statements)

References 17 publications

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“…Biodegradable synthetic materials have been widely studied because of their relative ease in processing and controllable mechanical properties [14–15], including polylactic acid (PLA)- or polyglycolic acid (PGA)-based scaffolds [14, 16–19]. However, poor cell adhesion, proliferation and matrix synthesis remain a challenge for these materials [20].…”

Section: Introductionmentioning

confidence: 99%

Dynamic culture conditions to generate silk-based tissue-engineered vascular grafts

Zhang¹,

Wang²,

Keshav³

et al. 2009

Biomaterials

144

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Tissue engineering is an alternative approach for the preparation of small-diameter (<6 mm) vascular grafts due to the potential to control thrombosis, anastomotic cellular hyperplasia and matrix production. This control also requires the maintenance of graft patency in vivo, appropriate mechanical properties and the formation of a functional endothelium. As a first step in generating such tissue-engineered vascular grafts (TEVG), our objective was to develop a tissue-engineered construct that mimicked the structure of blood vessels using tubular electrospun silk fibroin scaffolds (ESFS) with suitable mechanical properties. Human coronary artery smooth muscle cells (HCASMCs) and human aortic endothelial cells (HAECs) were sequentially seeded onto the luminal surface of the tubular scaffolds and cultivated under physiological pulsatile flow. The results demonstrated that TEVGs under dynamic flow conditions had better outcome than static culture controls in terms of cell proliferation and alignment, ECM production and cell phenotype based on transcript and protein level assessments. The metabolic activity of HCASMCs present in the TEGs indicated the advantage of dynamic flow over static culture in effective nutrient and oxygen distribution to the cells. A matrigel coating as a basement membrane mimic for ECM significantly improved endothelium coverage and retention under physiological shear forces. The results demonstrate the successful integration of vascular cells into silk electrospun tubular scaffolds as a step toward the development of a TEVG similar to native vessels in terms of vascular cell outcomes and mechanical properties.

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Section: Introductionmentioning

confidence: 99%

Dynamic culture conditions to generate silk-based tissue-engineered vascular grafts

Zhang¹,

Wang²,

Keshav³

et al. 2009

Biomaterials

144

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“…For blood contacting applications, the poly (d,l-lactide) would presumably hold an advantage over the more crystalline poly (1-lactide) and poly (glycolide) because amorphous polymers have been suggested to be less reactive to platelets than crystalline polymers [521. Poly (d,l-lactide) has good biocompatibility [47,48,53] because it degrades into lactic acid, a natural metabolite of glycolysis via hydrolytic chain scission [54].…”

Section: Prevention Of Graft Thrombosismentioning

confidence: 99%

“…Diffusion based controlled antithrombotic release can be achieved by incorporation of a drug loaded polymer matrix within the vascular graft. Degradable polymers are good candidates for controlled release matrices in vascular grafts because.they facilitate endothelial healing by acting as a temporary scaffold for cellular ingrowth [47][48][49]. While numerous types of degradable polymers have been used for the controlled release of pharmacological agents [50], lactide and glycolide polyesters (as well as their copolymers) have been the most thoroughly investigated [51].…”

Section: Prevention Of Graft Thrombosismentioning

confidence: 99%

Effect of Controlled Local Acetylsalicylic Acid Release on in vitro Platelet Adhesion to Vascular Grafts

1994

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Thrombosis is the most serious acute problem for small diameter arterial bypass grafts. In this research, small diameter expanded polytetrafluoroethylene (e-PTFE) vascular grafts were coated with acetylsalicylic acid (ASA) loaded poly (d,l-lactide) (PLA) by a solvent casting method. The feasibility and efficacy of this approach were evaluated by ASA release studies and platelet adhesion tests. First, the ASA release kinetics were evaluated from the ASA/PLA coated vascular grafts in an in vitro steady flow loop model. ASA release was measured by a spectrophotometric technique. Finally, the efficacy of local ASA release to reduce in vitro canine platelet adhesion to grafts was determined with epifluorescent video microscopy and quantitative image analysis. The steady state release rates from the 5%, 10%, and 15% ASA/PLA coated grafts were 13.2 x 10(-5), 32.0 x 10(-5), and 41.5 x 10(-5) micrograms/cm2.sec, respectively. Platelet adhesion to 10% and 15% ASA/PLA coated grafts was reduced with respect to the control and 5% grafts for 10 days. Platelet adhesion to 5% ASA/PLA coated grafts was reduced with respect to controls at 2 and 10 days, but not initially.

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“…Biodegradable synthetic materials have been used, because they are relatively easy to process and often match the required mechanical properties [4,5]. Many polylactide-or polyglycolide-based scaffolds have thus been produced [4,[6][7][8][9], but achieving the necessary compliance, cell adhesion, proliferation and matrix synthesis is still a challenge [10].…”

Section: Introductionmentioning

confidence: 99%

Physical characterization of vascular grafts cultured in a bioreactor

et al. 2006

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Tubular scaffolds of collagen and elastin (weight ratio 1:1) with interconnected pores were prepared by freeze drying and crosslinked with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) in the presence or absence of a Jeffamine spacer (poly(propylene glycol)-bis-(2-aminopropyl ether), J230). The crosslinked and uncrosslinked matrices had porosities of 90% and average pore sizes of 131-151 microm. Smooth muscle cells (SMC) were cultured in the crosslinked and uncrosslinked tubular scaffolds under pulsatile flow conditions (mean flow rate 9.6 ml/min, 120 beats/min, pressure 80-120 mmHg). All the constructs could withstand cyclic mechanical strain in the absence of any mechanical support without cracking or suffering permanent deformation. After 7d, SMC were homogeneously distributed throughout the uncrosslinked and EDC/NHS crosslinked constructs, whereas hardly any cell was observed on the luminal side of J230/EDC/NHS crosslinked matrices. Considering the better mechanical performance of EDC/NHS crosslinked matrices compared to non-crosslinked constructs after 7d of culture, SMC were dynamically cultured in the former scaffolds for 14d. During this period, the high strain stiffness of the constructs increased more than two-fold to 38+/-2 kPa, whereas the low strain stiffness doubled to 8+/-2 kPa. The yield stress and yield strain were 30+/-10 kPa and 120+/-20%, respectively. SMC were homogeneously distributed throughout the EDC/NHS crosslinked collagen/elastin constructs and collagen fibres tended to orient in the circumferential direction.

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A two-ply artificial blood vessel of polyurethane and poly(L-lactide)

Cited by 28 publications

References 17 publications

Dynamic culture conditions to generate silk-based tissue-engineered vascular grafts

Dynamic culture conditions to generate silk-based tissue-engineered vascular grafts

Effect of Controlled Local Acetylsalicylic Acid Release on in vitro Platelet Adhesion to Vascular Grafts

Physical characterization of vascular grafts cultured in a bioreactor

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