A two-step protocol for shoot regeneration from hypocotyl explants of oilseed rape and its application for Agrobacterium-mediated transformation

Tang, Guixiang; Knecht, Kerstin; Yang, X. F.; Qin, Ying; Zhou, Weijun; Cai, Daguang

doi:10.1007/s10535-011-0003-0

Cited by 11 publications

(8 citation statements)

References 28 publications

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“…Cotyledonary node explants infected for 15 min obtained the highest transformation efficiency in this study. In contrast to this result, Mashayekhi et al (2008), Khan et al (2010) and Tang et al (2011) reported that 5-10 min of infection showed the maximum transformation efficiency.…”

Section: Discussionmentioning

confidence: 72%

Establishment of a High-efficiency Genetic Transformation System of Cucumber (Cucumis sativus) using Csexpansin 10 (CsEXP10) Gene

Sun¹,

Luo²,

Li³

et al. 2017

IJAB

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Cucumber is one of the most important vegetable crops in the world. To establish an efficient genetic transformation system in cucumber, the role of different factors such as pre-cultivation time, acetosyringone concentration, infection time and cocultivation time that can influence the transformation, was evaluated. In addition, Csexpansin 10 (CsEXP10) gene was transformed into the cucumber genome to produce transgenic lines. The results showed that various optimal parameters such as 100 mg L -1 of kanamycin concentration for selection of transformants, 2 d of pre-cultivation time, 100 μmol L -1 of acetosyringone concentration, 15 min of infection time and 2 d of co-cultivation time were obtained using cotyledonary node explants. The rooting frequency observed on Murashige and Skoog (MS) medium supplemented with 0.2 mg L -1 indole acetic acid and 400 mg L -1 cefotaxime was found to be 100.00%. The positive transgenic cucumber lines were identified using PCR analysis and GUS staining assay. It suggests that the genetic transformation system developed using cotyledonary node explants is efficient and successful in cucumber.

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Section: Discussionmentioning

confidence: 72%

Establishment of a High-efficiency Genetic Transformation System of Cucumber (Cucumis sativus) using Csexpansin 10 (CsEXP10) Gene

Sun¹,

Luo²,

Li³

et al. 2017

IJAB

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“…Silver thiosulphate, an ethylene inhibitor, is often used to block the action of this hormone. Application of this compound has been shown to increase the stable transformation efficiency of black cherry (Liu and Pijut 2010) and oilseed rape (Tang et al 2011). Similarly, the presence of STS in selection media used for chestnut somatic embryos improved the transformation efficiency, although differences were not significant.…”

Section: Discussionmentioning

confidence: 97%

Agrobacterium-mediated transformation of European chestnut somatic embryos with a Castanea sativa (Mill.) endochitinase gene

et al. 2016

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Chestnut blight, caused by Cryphonectria parasitica, is a severe disease that has devastated chestnut stands in North America and Europe. Genes encoding hydrolytic enzymes such as chitinases, which can degrade fungal cell wall components, are attractive candidates for improving disease resistance. This report describes a reliable and efficient protocol for the Agrobacterium-mediated transformation of somatic embryos of European chestnut with the endogenous CsCh3 gene that codes for chitinase. The transformation efficiency, determined on the basis of the fluorescence of surviving explants, was genotype-dependent. Although somatic embryos of all three lines evaluated were transformed, the best results were obtained with somatic embryos derived from line CI-9 (20%). The addition of silver thiosulphate (20 or 40 μM) improved the transformation efficiency of somatic embryos derived from lines CI-3 and CI-9, although the differences were not significant. A total of 88 independent transformed lines were obtained. The presence of transgenes was confirmed by green fluorescent protein (GFP) expression, PCR and Southern blot analysis. Transgenic lines were maintained by secondary embryogenesis or cryopreservation following vitrification procedures. Maturation and germination of transformed somatic embryos yielded transgenic plants. Fluorescence indicating overexpression of the transgenes was observed in somatic embryos and also in shoots and leaves. No phenotypic differences were found relative to control plants, suggesting a lack of any cytotoxic effects of the GFP.

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“…Brassica carinata A. Br. (Yang et al, 1991), Arabidosis thaliana (Gendreau et al, 1997), Brassica napus L. (Tang et al, 2011), Brassica oleracea cv. (Gambhir et al, 2017) Zygophyllum potaninii (Bayarmaa et al, 2018), Solanum quitoense (Gutiérrez et al, 2019).…”

Section: Direct Organogenesismentioning

confidence: 99%

Seed Germination and Micropropagation Studies of Mammea Suriga Kosterm

Shastri¹,

Krishna²,

Sheshagiri³

2020

J microb biotech food sci

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Mammea suriga Kosterm. is a RET species distributed in Malnad region of the Western Ghats, which is over exploited for flowers, seeds and timbers, hence the population is declined. The study was conducted to evaluate the germination potency of the seeds in both in vivo and in vitro conditions to derive a regeneration protocol for the ex situ conservation. Among the pre-treated seeds, the decoated seeds pre-treated with 0.5 mg L-1 NAA for 24 h resulted in 86 % of germination. In in vitro condition, culture of decoated seeds on MS medium supplemented with 0.5 mg L-1 NAA and 0.1 mg L-1 TDZ resulted 91.67 % of germination. Micropropagation studies showed that the interaction of 2-3 mg L-1 BAP and 0.2 mg L-1 TDZ induced multiple shoots from cotyledonary and hypocotyl explants. Mass of callus induced from the leaf explants at the concentration of 1 mg L-1 2,4-D and 3 mg L-1 BAP. Later on the photosynthetic callus, organise in to shoot buds at the concentration of 3 mg L-1 BAP and 0.2 mg L-1 TDZ. The excised micro shoots showed rhizogenesis on the media fortified with 1.5 mg L-1 IBA. The regenerants derived from both direct and indirect organogenesis exhibited similar morphological features.

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A two-step protocol for shoot regeneration from hypocotyl explants of oilseed rape and its application for Agrobacterium-mediated transformation

Cited by 11 publications

References 28 publications

Establishment of a High-efficiency Genetic Transformation System of Cucumber (Cucumis sativus) using Csexpansin 10 (CsEXP10) Gene

Establishment of a High-efficiency Genetic Transformation System of Cucumber (Cucumis sativus) using Csexpansin 10 (CsEXP10) Gene

Agrobacterium-mediated transformation of European chestnut somatic embryos with a Castanea sativa (Mill.) endochitinase gene

Seed Germination and Micropropagation Studies of Mammea Suriga Kosterm

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