A type III-like restriction endonuclease functions as a major barrier to horizontal gene transfer in clinical
            <i>Staphylococcus aureus</i>
            strains

Corvaglia, Anna Rita; François, Patrice; Hernández, David; Perron, Karl; Linder, Patrick; Schrenzel, Jacques

doi:10.1073/pnas.1000489107

Cited by 113 publications

(122 citation statements)

References 43 publications

Supporting

Mentioning

111

Contrasting

Unclassified

Order By: Relevance

“…This may be particularly relevant for the CC398 lineage. Our sequencing findings clearly indicate that CC398 does not harbor any genes for a type IV RM system similar to those previously described (Corvaglia et al, 2010). In addition, the 296 bp fragment of the sauI-hsdSI gene, a reliable discriminative marker of S. aureus CC398 (Stegger et al, 2011), is present and strictly conserved in our isolates.…”

Section: Discussionsupporting

confidence: 60%

“…In addition, the 296 bp fragment of the sauI-hsdSI gene, a reliable discriminative marker of S. aureus CC398 (Stegger et al, 2011), is present and strictly conserved in our isolates. However, our transformation and transduction experiments clearly indicate that the presence of this sauIhsdSI gene, a component of a type I RM system, is not associated with protection against uptake of foreign DNA (Waldron and Lindsay, 2006;Corvaglia et al, 2010).…”

Section: Discussionmentioning

confidence: 91%

See 1 more Smart Citation

Analysis of prophages harbored by the human-adapted subpopulation of Staphylococcus aureus CC398

Mee-Marquet

Corvaglia

Valentin

et al. 2013

Infection, Genetics and Evolution

Self Cite

View full text Add to dashboard Cite

a b s t r a c tStaphylococcus aureus clonal complex 398 is a livestock-associated pathogen that poses a worldwide threat because of its ability to colonize and infect both humans and animals. We used high-resolution whole-genome microarrays, prophage profiling, immune evasion cluster characterization and wholegenome sequencing to investigate the roles of prophages in the emerging human-adapted subpopulation of CC398 that has been associated with invasive infections in humans living in animal-free environments.We characterized one phage and two prophages specifically harbored by CC398 isolates belonging to the emerging subpopulation. We introduced the phage into permissive prophage-free isolates. We investigated the effects of lysogeny on the host ability to resist further phage infection and transformation, to acquire the capacity to invade human cells, and to express virulence factors encoded by prophages. We report evidence of a defective /MR11-like helper prophage, named StauST398-5pro, specifically associated with the emerging non-LA CC398 subpopulation. StauST398-5pro confers substantial protection against horizontal genetic transfer to its host. It interacts with a human-associated b-converting prophage encoding immune-modulating proteins such that virulence genes are expressed during stress situations.Our findings provide insight into the role of phages in the expression of virulence and in the spread of genetic information among new host-adapted S. aureus isolates. We demonstrate that functional prophage elements can condition host specificity and confer new virulence traits on emerging intra-species clones of bacteria.

show abstract

Section: Discussionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 91%

Analysis of prophages harbored by the human-adapted subpopulation of Staphylococcus aureus CC398

Mee-Marquet

Corvaglia

Valentin

et al. 2013

Infection, Genetics and Evolution

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, it is not in agreement with the observation that pairs of genomes encoding cognate R-M systems coexchange more DNA. It is also hardly reconcilable with the notorious deleterious effect of R-M systems on bacterial genetic transformation in the laboratory (35). Although R-M systems have been shown to favor intragenomic HR events (12), the overall effect of R-M systems on genetic exchange is to decrease both HR and HGT between bacteria encoding noncognate R-M systems.…”

Section: Discussionmentioning

confidence: 87%

Regulation of genetic flux between bacteria by restriction–modification systems

Oliveira

Touchon

Rocha

2016

Proc. Natl. Acad. Sci. U.S.A.

144

150

View full text Add to dashboard Cite

Restriction-modification (R-M) systems are often regarded as bacteria's innate immune systems, protecting cells from infection by mobile genetic elements (MGEs). Their diversification has been recently associated with the emergence of particularly virulent lineages. However, we have previously found more R-M systems in genomes carrying more MGEs. Furthermore, it has been suggested that R-M systems might favor genetic transfer by producing recombinogenic double-stranded DNA ends. To test whether R-M systems favor or disfavor genetic exchanges, we analyzed their frequency with respect to the inferred events of homologous recombination and horizontal gene transfer within 79 bacterial species. Genetic exchanges were more frequent in bacteria with larger genomes and in those encoding more R-M systems. We created a recognition target motif predictor for Type II R-M systems that identifies genomes encoding systems with similar restriction sites. We found more genetic exchanges between these genomes, independently of their evolutionary distance. Our results reconcile previous studies by showing that R-M systems are more abundant in promiscuous species, wherein they establish preferential paths of genetic exchange within and between lineages with cognate R-M systems. Because the repertoire and/or specificity of R-M systems in bacterial lineages vary quickly, the preferential fluxes of genetic transfer within species are expected to constantly change, producing time-dependent networks of gene transfer.homologous recombination | horizontal gene transfer | bacterial evolution

show abstract

“…Thus, type I gene complexes appear to function in controlling the evolution of S. aureus strains. Recent studies of type III-like enzymes also revealed the role of these REases as a major barrier to HGT in clinical strains of methicillin-resistant S. aureus (115). Strains deficient in these enzymes were hypersusceptible to the horizontal acquisition of DNA from other species, such as E. coli, and could easily acquire a vancomycin resistance gene from enterococci (115).…”

Section: Immigration Control Maintenance Of Species Identity and Comentioning

confidence: 99%

“…Recent studies of type III-like enzymes also revealed the role of these REases as a major barrier to HGT in clinical strains of methicillin-resistant S. aureus (115). Strains deficient in these enzymes were hypersusceptible to the horizontal acquisition of DNA from other species, such as E. coli, and could easily acquire a vancomycin resistance gene from enterococci (115). Subsequent studies, however, indicated that the type III-like enzyme is actually a type IV REase recognizing 5-methylcytosine/5-hydroxymethyl cytosine-modified DNA (116).…”

Section: Immigration Control Maintenance Of Species Identity and Comentioning

confidence: 99%

Diverse Functions of Restriction-Modification Systems in Addition to Cellular Defense

Vasu

Nagaraja

2013

Microbiol Mol Biol Rev

523

462

View full text Add to dashboard Cite

SUMMARY Restriction-modification (R-M) systems are ubiquitous and are often considered primitive immune systems in bacteria. Their diversity and prevalence across the prokaryotic kingdom are an indication of their success as a defense mechanism against invading genomes. However, their cellular defense function does not adequately explain the basis for their immaculate specificity in sequence recognition and nonuniform distribution, ranging from none to too many, in diverse species. The present review deals with new developments which provide insights into the roles of these enzymes in other aspects of cellular function. In this review, emphasis is placed on novel hypotheses and various findings that have not yet been dealt with in a critical review. Emerging studies indicate their role in various cellular processes other than host defense, virulence, and even controlling the rate of evolution of the organism. We also discuss how R-M systems could have successfully evolved and be involved in additional cellular portfolios, thereby increasing the relative fitness of their hosts in the population.

show abstract

A type III-like restriction endonuclease functions as a major barrier to horizontal gene transfer in clinical Staphylococcus aureus strains

Cited by 113 publications

References 43 publications

Analysis of prophages harbored by the human-adapted subpopulation of Staphylococcus aureus CC398

Analysis of prophages harbored by the human-adapted subpopulation of Staphylococcus aureus CC398

Regulation of genetic flux between bacteria by restriction–modification systems

Diverse Functions of Restriction-Modification Systems in Addition to Cellular Defense

Contact Info

Product

Resources

About