A unique Golgi apparatus distribution may be a marker for osteogenic differentiation of hDP‐MSCs

Kasap, Murat; Karaöz, Erdal; Akpınar, Gürler; Aksoy, Aziz; Erman, Gülay

doi:10.1002/cbf.1776

Cited by 7 publications

(1 citation statement)

References 30 publications

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“…Sperm samples were placed on glass cover slips and were fixed with formaldehyde and permeabilized with 0.5% Triton X-100 as described in Kasap et al, (Kasap, 2011). Cover slips were mounted in Mowiol before analysis.…”

Section: Immunofluorescence Stainingmentioning

confidence: 99%

Aneuploidy Rate, DNA Fragmentation, Tubulin and Centrin Content in Different Groups of Male Infertility

Budak¹,

Ceylan²,

Toprak³

et al. 2020

JMPB

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In this study, we aimed to compare DNA fragmentation ratios, centrosomal protein concentrations (centrin and tubulin) and numerical chromosomal abnormalities between four groups including asthenozoospermia, teratozoospermia, oligoasthenozoospermia and normozoospermia patients, and to emphasize the necessity of investigating genetic and protein content of the spermatozoa also in patients with a semen analysis within normal reference range. Evaluation of semen analysis of the patients was performed based on WHO 2010 criteria. As a result of the statistical analysis, it was observed that the sperm DNA fragmentation rate in the normozoospermia group (6.50 ± 3.46%) was significantly lower than those in the teratozoospermia (19.69 ± 9.86%) and oligoasthenoteratozoospermia group (32.47± 14.13%) (p<0.001). For centrin and tubulin proteins, highest concentrations were observed in normozospermia group (p<0.05). When incidence of numerical abnormalities were compared between four groups, it was found that there were statistically significant differences between groups in terms of nullisomies X, Y and 13, and 21 (p<0.05). In conclusion, we suggest that abnormalities in semen parameters seem to be associated with chromosomal abnormalities; and sperm aneuploidy rate is also at a considerable level even in normozoospermic patients.

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Section: Immunofluorescence Stainingmentioning

confidence: 99%

Aneuploidy Rate, DNA Fragmentation, Tubulin and Centrin Content in Different Groups of Male Infertility

Budak¹,

Ceylan²,

Toprak³

et al. 2020

JMPB

View full text Add to dashboard Cite

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Proteomics Approaches in the Identification of Molecular Signatures of Mesenchymal Stem Cells

Xiao

Chen

2012

Mesenchymal Stem Cells - Basics and Clinical Application I

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Mesenchymal stem cells (MSCs) are undifferentiated, multi-potent stem cells with the ability to renew. They can differentiate into many types of terminal cells, such as osteoblasts, chondrocytes, adipocytes, myocytes, and neurons. These cells have been applied in tissue engineering as the main cell type to regenerate new tissues. However, a number of issues remain concerning the use of MSCs, such as cell surface markers, the determining factors responsible for their differentiation to terminal cells, and the mechanisms whereby growth factors stimulate MSCs. In this chapter, we will discuss how proteomic techniques have contributed to our current knowledge and how they can be used to address issues currently facing MSC research. The application of proteomics has led to the identification of a special pattern of cell surface protein expression of MSCs. The technique has also contributed to the study of a regulatory network of MSC differentiation to terminal differentiated cells, including osteocytes, chondrocytes, adipocytes, neurons, cardiomyocytes, hepatocytes, and pancreatic islet cells. It has also helped elucidate mechanisms for growth factor-stimulated differentiation of MSCs. Proteomics can, however, not reveal the accurate role of a special pathway and must therefore be combined with other approaches for this purpose. A new generation of proteomic techniques have recently been developed, which will enable a more comprehensive study of MSCs.

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The influence of nanotopography on organelle organization and communication

et al. 2016

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A unique Golgi apparatus distribution may be a marker for osteogenic differentiation of hDP‐MSCs

Cited by 7 publications

References 30 publications

Aneuploidy Rate, DNA Fragmentation, Tubulin and Centrin Content in Different Groups of Male Infertility

Aneuploidy Rate, DNA Fragmentation, Tubulin and Centrin Content in Different Groups of Male Infertility

Proteomics Approaches in the Identification of Molecular Signatures of Mesenchymal Stem Cells

The influence of nanotopography on organelle organization and communication

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