A unique lectin composing of fibrinogen-like domain from Fenneropenaeus merguiensis contributed in shrimp immune defense and firstly found to mediate encapsulation

Senghoi, Wilaiwan; Thongsoi, Ratiporn; Yu, Xiao‐Qiang; Runsaeng, Phanthipha; Utarabhand, Prapaporn

doi:10.1016/j.fsi.2019.06.009

Cited by 16 publications

(3 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Because the Ca 2+ ion is not directly participating in ligand binding, it is possible that the Ca 2+ ion may only have structural and stability role for PmFREP. There are reports that other shrimp fibrinogen-related lectins require Ca 2+ ion for ligand binding [29][30][31][32][33] . However, several ficolins are also capable of Ca 2+ -independent ligand binding 19,[34][35][36] .…”

Section: Discussionmentioning

confidence: 99%

Biochemical and structural characterization of a recombinant fibrinogen-related lectin from Penaeus monodon

Singrang

Laophetsakunchai

Tran

et al. 2021

Sci Rep

View full text Add to dashboard Cite

Fibrinogen-related lectins are carbohydrate-binding proteins of the innate immune system that recognize glycan structures on microbial surfaces. These innate immune lectins are crucial for invertebrates as they do not rely on adaptive immunity for pathogen clearance. Here, we characterize a recombinant fibrinogen-related lectin PmFREP from the black tiger shrimp Penaeus monodon expressed in the Trichoplusia ni insect cell. Electron microscopy and cross-linking experiments revealed that PmFREP is a disulfide-linked dimer of pentamers distinct from other fibrinogen-related lectins. The full-length protein binds N-acetyl sugars in a Ca2+ ion-independent manner. PmFREP recognized and agglutinated Pseudomonas aeruginosa. Weak binding was detected with other bacteria, including Vibrio parahaemolyticus, but no agglutination activity was observed. The biologically active PmFREP will not only be a crucial tool to elucidate the innate immune signaling in P. monodon and other economically important species, but will also aid in detection and prevention of shrimp bacterial infectious diseases.

show abstract

Section: Discussionmentioning

confidence: 99%

Biochemical and structural characterization of a recombinant fibrinogen-related lectin from Penaeus monodon

Singrang

Laophetsakunchai

Tran

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…However, these proteins are known to have similar functions to lectins that regulate AMP activation. A broad‐spectrum FREP with CTL function was characterized in P. merguiensis that promoted encapsulation of V. parahaemolyticus by haemocytes as a cellular immune response, hence enacting the role of PRR in shrimp immune defence 221 . The FREP from M. rosenbergii also contributed to the host's innate immunity by acting as a PRR against bacterial pathogens as demonstrated by the clearance of V. parahaemolyticus 222 .…”

Section: Prrs In Crustaceansmentioning

confidence: 99%

“…A broad-spectrum FREP with CTL function was characterized in P. merguiensis that promoted encapsulation of V. parahaemolyticus by haemocytes as a cellular immune response, hence enacting the role of PRR in shrimp immune defence. 221 The FREP from M. rosenbergii also contributed to the host's innate immunity by acting as a PRR against bacterial pathogens as demonstrated by the clearance of V. parahaemolyticus. 222 FREPs such as the tachylectins from the horseshoe crab Tachypleus tridentatus, ficolin-like proteins from crayfish P. leniusculus, and fibrinogen-like protein from P. clarkii has been characterized to show Ca 2+ -dependent agglutination activities against bacteria such as A. hydrophila, E. coli, M. lysodeikticus and S. aureus.…”

Section: Fibrinogen-related Proteinsmentioning

confidence: 99%

The role of pattern recognition receptors in crustacean innate immunity

Patnaik

Baliarsingh

Sarkar³

et al. 2023

Reviews in Aquaculture

View full text Add to dashboard Cite

Crustacean species are among the most valued seafood with socioeconomic and ecological implications in the global fisheries sector. Microbial infections to the farming stock due to continued expansion and aquaculture puts global crustacean production at risk. Understanding and reviewing the complex innate immune system of crustaceans would be pivotal in formulating informed decisions for the control and management of epidemic diseases, thus making the global crustacean aquaculture industry sustainable. Under the purview of innate immunity in crustaceans, pattern recognition receptors (PRRs) represent the sensors capable of discriminating non‐self pathogenic modules and invoking the activation of immune responses. The immunity to infections can be manifested at cellular (via phagocytosis, apoptosis, nodulation and encapsulation) and humoral (effected by prophenoloxidase cascade, lectins, antimicrobial proteins) levels leading to the elimination of pathogens or subversion of the pathogenic implications. In this review, we discuss the participatory role of 12 types of PRRs identified from crustacean species with an exhaustive focus on structural and functional specificities in binding to pathogens and possible execution of the immune activity. Moreover, we have elaborated on the PRRs‐associated or possible signalling cascades (Toll‐NFκB, IMD‐NFκB and JAK‐STAT) in crustacean species that could bridge our understanding of the resistance or susceptibility of the host to varied pathogenic infections thus providing clues to the regulatory mechanisms involved in pathogenesis of infectious diseases and mechanisms of immunity.

show abstract

Lectins in Penaeid Shrimps: Purification, Characterization, and Biological Significance

Johnson,

Thiraviam,

Chellathangam

et al. 2022

Aquatic Lectins

View full text Add to dashboard Cite

A unique lectin composing of fibrinogen-like domain from Fenneropenaeus merguiensis contributed in shrimp immune defense and firstly found to mediate encapsulation

Cited by 16 publications

References 44 publications

Biochemical and structural characterization of a recombinant fibrinogen-related lectin from Penaeus monodon

Biochemical and structural characterization of a recombinant fibrinogen-related lectin from Penaeus monodon

The role of pattern recognition receptors in crustacean innate immunity

Lectins in Penaeid Shrimps: Purification, Characterization, and Biological Significance

Contact Info

Product

Resources

About