A universal label-free fluorescent aptasensor based on Ru complex and quantum dots for adenosine, dopamine and 17β-estradiol detection

Huang, Hailiang; Shi, Shuo; Gao, Xing; Gao, Ruru; Zhu, Ying; Wu, Xuewen; Zang, Ruimin; Yao, Tianming

doi:10.1016/j.bios.2015.12.024

Cited by 101 publications

(33 citation statements)

References 64 publications

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“…Hormones, which are the signaling molecules secreted from glands, play an important role in the regulation of human physiological activities . Among them, multiple steroid hormones (including androgens, estrogens, and progestogens) exhibit synergistic effects on the body for its development, growth, and functions . It has been suggested that the occurrence and development of many deadly diseases are closely related to the deregulation of multiple hormones, rather than an individual steroid hormone.…”

Section: Introductionmentioning

confidence: 99%

“…[1] Amongt hem, multiple steroid hormones (including androgens, estrogens, and progestogens) exhibit synergistic effects on the body for its development, growth, and functions. [2][3][4][5] It has been suggested that the occurrencea nd development of many deadly diseases are closely related to the deregulation of multiple hormones, rather than an individual steroidh ormone. For example, previous studies have strongly suggested that the synchronousv ariation of estrogen and progestogenl evels is relatedt ot he development of gynecological cancers such as ovarian, uterine,a nd breast cancer.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous Visualization and Quantitation of Multiple Steroid Hormones Based on Signal‐Amplified Biosensing with Duplex Molecular Recognition

Tan

et al. 2017

Chemistry A European J

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The simultaneous quantitation of multiple steroid hormones in real time is of great importance in medical diagnosis. In this study, a portable hormone biosensor based on duplex molecular recognition coupled with a signal-amplified substrate was successfully developed for the simultaneous visualization and quantitation of multiple steroid hormones. Aptamer-functionalized upconversion nanoparticles (UCNPs) with different emission peaks are immobilized on the photonic crystal (PC) substrate as the nanoprobes, leading to the specific and simultaneous assay of multiple steroid hormones. Coupled with the luminescence-enhanced effect of the PC substrate, nanomolar quantification limits of multiple hormones are achieved. This well-designed biosensor is also promising in the quantification of multiple hormones in serum samples. The amplified luminescence signals can be visualized with the naked eye and captured by an unmodified phone camera. This hormone quantitation biosensor exhibits the advantages of multi-detection, visualization, high sensitivity, and selectivity for potential applications in clinical disease diagnosis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Simultaneous Visualization and Quantitation of Multiple Steroid Hormones Based on Signal‐Amplified Biosensing with Duplex Molecular Recognition

Tan

et al. 2017

Chemistry A European J

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“…67 Based on the specific binding properties of an aptamer, Yao and co-workers reported a method used to detect three different chemicals; adenosine, dopamine and 17b-estradiol. 68 In the study, Ru complexes and quantum dots (QDs) were used as fluorescent probes. Ru complexes can quench the fluorescence of QDs, and QDs also preferentially bind to the adaptive DNA, resulting in an increase of the fluorescence of QDs.…”

Section: High Affinity and Specificitymentioning

confidence: 99%

Aptamer-functionalized nanomaterials for biological applications

Ren

Lu³

et al. 2020

Mater. Chem. Front.

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“…Besides the biosensor-based techniques based on colorimetric, 17,18 fluorogenic, [19][20][21] and electrochemical properties, 22 the magnetic relaxation switch (MRS) sensor has attracted increasing attention. In MRS detection, the presence of a target substance will change the dispersed and aggregated states of nanoparticles (NPs), resulting in the change of the spin-spin relaxation time (T 2 ) of surrounding water protons.…”

Section: Introductionmentioning

confidence: 99%

A magnetic relaxation switch sensor for determination of 17β‐estradiol in milk and eggs based on aptamer‐functionalized Fe₃O₄@Au nanoparticles

et al. 2021

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BACKGROUND: A simple and rapid detection method for 17⊎-estradiol (E 2 ) in complex food matrix is greatly desirable. A magnetic relaxation switch (MRS) sensor for detecting E 2 based on the aptamer-functionalized gold-coated iron oxide (Fe 3 O 4 @Au) nanocomposite was designed in this study. Fe 3 O 4 @Au nanoparticles (NPs) played as a 'switch' between dispersed and aggregated states, while aptamer served as the recognition unit.RESULTS: According to the sensing effect of monocomponent relaxation time (T 2W ) for E 2 , the volume ratio of aptamers to Fe 3 O 4 @Au, the sodium chloride (NaCl) concentration, the concentration of Fe 3 O 4 @Au@Apt, and reaction time were optimized to be 4:1, 0.03 mol L -1 , 4 ∼mol L -1 and 15 min, respectively. For the analysis of food sample, the E 2 was quantified over a concentration range of 1 to 100 nmol L -1 with a detection limit of 7.6 nmol L -1 for milk samples, while a linearity range of 20 to 100 nmol L -1 and a detection limit of 8.57 nmol L -1 for egg samples.CONCLUSION: These results exhibited that the MRS sensor could be a promising platform for the rapid detecting of E 2 in food sample.

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A universal label-free fluorescent aptasensor based on Ru complex and quantum dots for adenosine, dopamine and 17β-estradiol detection

Cited by 101 publications

References 64 publications

Simultaneous Visualization and Quantitation of Multiple Steroid Hormones Based on Signal‐Amplified Biosensing with Duplex Molecular Recognition

Simultaneous Visualization and Quantitation of Multiple Steroid Hormones Based on Signal‐Amplified Biosensing with Duplex Molecular Recognition

Aptamer-functionalized nanomaterials for biological applications

A magnetic relaxation switch sensor for determination of 17β‐estradiol in milk and eggs based on aptamer‐functionalized Fe₃O₄@Au nanoparticles

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A universal label-free fluorescent aptasensor based on Ru complex and quantum dots for adenosine, dopamine and 17β-estradiol detection

Cited by 101 publications

References 64 publications

Simultaneous Visualization and Quantitation of Multiple Steroid Hormones Based on Signal‐Amplified Biosensing with Duplex Molecular Recognition

Simultaneous Visualization and Quantitation of Multiple Steroid Hormones Based on Signal‐Amplified Biosensing with Duplex Molecular Recognition

Aptamer-functionalized nanomaterials for biological applications

A magnetic relaxation switch sensor for determination of 17β‐estradiol in milk and eggs based on aptamer‐functionalized Fe3O4@Au nanoparticles

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A magnetic relaxation switch sensor for determination of 17β‐estradiol in milk and eggs based on aptamer‐functionalized Fe₃O₄@Au nanoparticles