A USPIO doped gel phantom for R2* relaxometry

Brown, G. A.; Cowin, Gary; Galloway, Graham J.

doi:10.1007/s10334-016-0576-x

Cited by 4 publications

(2 citation statements)

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“…However, the application of tissue engineering grafts is still a long way to clinics, facing various challenges [3,4]. In addition to the limitations and deficiencies of the artificial implant itself, it is largely due to the inability to longitudinally and non-destructively track the degradation of biological implementation, and the microenvironment in the human body is dynamic and complex [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of 3D porous conductive scaffolds with magnetic resonance enhancement in tissue engineering

Chen

Feng³

et al. 2019

Biomed. Mater.

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Magnetic resonance imaging (MRI), as a diagnostic tool in tissue engineering, has received widespread attention because of its ability to consistently provide degradation and absorption of implants in vivo. For some specific human tissues and organs, such as nerves, muscles and myocardium, their regeneration requires tissue engineering scaffolds have a good electrical conductivity. Graphene oxide (GO) has been extensively studied as a conductive biomaterial having mechanical reinforcement. Based on the above, we propose an MRI conductive scaffold containing gelatin (Gel)/gelatin-polycaprolactone (Gel-PCL)/ultra-small paramagnetic iron oxide (USPIO)/graphene oxide (GO) (Gel/Gel-PCL/USPIO/GO). Their physical and chemical properties as well as biocompatibility are measured in vitro. The purpose of doping USPIO was developed for non-invasive monitoring of tissue engineered implants and tissue reconstruction. Functional modification of GO to match electrophysiological requirement. Co-culture with bone marrow mesenchymal stem cells showed good biocompatibility. Blood experiments have also demonstrated the feasibility of scaffolds as tissue engineered implants. The USPIO-labeled conductive scaffold, as an effective image-guided and electrically stimulating implant, appears to be a reconstruction platform for specific tissues and organs.

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Section: Introductionmentioning

confidence: 99%

Preparation and characterization of 3D porous conductive scaffolds with magnetic resonance enhancement in tissue engineering

Chen

Feng³

et al. 2019

Biomed. Mater.

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“…Gold-standard measurements were obtained using a single-slice inversion-recovery spin-echo pulse sequence (TR/TE/TI = 1550/11/[30, 330, 730, 1130, 1530] ms). Since there is no accepted reference method for measuring R 2 * and the multi-echo spoiled gradient echo sequence is regarded as the standard method, we did not evaluate this sequence in vitro; however, phantom experiments can be used to evaluate repeatability, reproducibility and relaxivity properties [30].…”

Section: Methodsmentioning

confidence: 99%

MRI Relaxometry for Quantitative Analysis of USPIO Uptake in Cerebral Small Vessel Disease

Thrippleton

Blair

Valdés-Hernández

et al. 2019

IJMS

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A protocol for evaluating ultrasmall superparamagnetic particles of iron oxide (USPIO) uptake and elimination in cerebral small vessel disease patients was developed and piloted. B1-insensitive R1 measurement was evaluated in vitro. Twelve participants with history of minor stroke were scanned at 3-T MRI including structural imaging, and R1 and R2* mapping. Participants were scanned (i) before and (ii) after USPIO (ferumoxytol) infusion, and again at (iii) 24–30 h and (iv) one month. Absolute and blood-normalised changes in R1 and R2* were measured in white matter (WM), deep grey matter (GM), white matter hyperintensity (WMH) and stroke lesion regions. R1 measurements were accurate across a wide range of values. R1 (p < 0.05) and R2* (p < 0.01) mapping detected increases in relaxation rate in all tissues immediately post-USPIO and at 24–30 h. R2* returned to baseline at one month. Blood-normalised R1 and R2* changes post-infusion and at 24–30 h were similar, and were greater in GM versus WM (p < 0.001). Narrower distributions were seen with R2* than for R1 mapping. R1 and R2* changes were correlated at 24–30 h (p < 0.01). MRI relaxometry permits quantitative evaluation of USPIO uptake; R2* appears to be more sensitive to USPIO than R1. Our data are explained by intravascular uptake alone, yielding estimates of cerebral blood volume, and did not support parenchymal uptake. Ferumoxytol appears to be eliminated at 1 month. The approach should be valuable in future studies to quantify both blood-pool USPIO and parenchymal uptake associated with inflammatory cells or blood-brain barrier leak.

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Quantitative MRI of diffuse liver diseases: techniques and tissue-mimicking phantoms

Tipirneni-Sajja

Brasher

Shrestha

et al. 2022

Magn Reson Mater Phy

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A USPIO doped gel phantom for R2* relaxometry

Abstract: The phantom provides a durable test object with controlled R2* relaxation behaviour, useful for a range of R2* relaxometry reference work.

Cited by 4 publications

References 50 publications

Preparation and characterization of 3D porous conductive scaffolds with magnetic resonance enhancement in tissue engineering

Preparation and characterization of 3D porous conductive scaffolds with magnetic resonance enhancement in tissue engineering

MRI Relaxometry for Quantitative Analysis of USPIO Uptake in Cerebral Small Vessel Disease

Quantitative MRI of diffuse liver diseases: techniques and tissue-mimicking phantoms

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