A validated capillary gas chromatography method for guaco (Mikania glomerata S.) quality control and rastreability: from plant biomass to phytomedicines

Abstract

A validated capillary gas chromatography method for guaco (Mikania glomerata S.) quality control and rastreability: from plant biomass to phytomedicines ABSTRACT: This work describes a full validation of a capillary gas chromatography analytical methodology using internal standardization for the quantification of coumarin (1,2-benzopyrone) in guaco (Mikania glomerata Spreng -Asteraceae) products: syrup, plant and its extract, including the stability study of the phytomedicine. For the analysis, it was used an HP-5 capillary column (30 m x 0.32 mm x 0.25 µm), hydrogen at a flow rate of 1.8 mL/min and the increasing temperature gradient was: 100 °C to 250 °C, 15 ºC/min. The temperature of injector (split 1:20) and detector were kept at 250 °C and 270 °C, respectively. The retention times of the standards for the above conditions were 2.86 minutes for 1, 2, 3, 4-tetramethylbenzene, 4.45 min for piperonal (internal standards), and 5.36 minutes for coumarin. After extraction procedure, the recovery of coumarin determined for plant raw material was 101.6 %, while for syrup it was 100.8 %. Detection and quantification limits were 0.5 µg/mL and 1.5 µg/mL, respectively. Precision was determined for all samples and the results were lower than 2.5 %. The total amount of coumarin in plant raw material, its extract and syrup were 0.38 % w/w, 1.33 mg/mL and 0.143 mg/mL, respectively.

Determinação do teor de cumarina no xarope de guaco armazenado em diferentes temperaturas

“…O gênero Mikania contém cerca de 300 espécies as quais são usadas em medicina popular (Agra et al, 2007;. O estudo fitoquímico de diversas espécies de Mikania conduziu ao isolamento de monoterpenos, lactonas sesquiterpênicas, diterpenos e cumarinas (Christo et al, 2006;Reis et al, 2008;Bolina et al, 2009;Bueno & Bastos, 2009).…”

Determinação de taninos e metilxantinas no guaraná em pó (Paullinia cupana Kunth, Sapindaceae) por cromatografia líquida de alta eficiência

“…Análises de controle de qualidade de matérias-primas vegetais e do fitoterápico são complexas, porém imprescindíveis para contribuir para a qualidade do medicamento (Ushirobira et al, 2004b;Bara et al, 2004;Bara et al, 2006;Chaves & Costa 2008;Engel et al, 2008;Paula et al, 2008;Alvarenga et al, 2009;Bueno & Bastos, 2009;Couto et al, 2009).…”

Mikania glomerata and M. laevigata: Clinical and Toxicological Advances

“…Thus, using different analytical procedures, the presence of a large number of compounds has been described, including stigmasterol, phytol, 1-ethoxy-1-phenylethanol, 4-hydroxy-3,5-dimethoxybenzaldehyde, hexanoic acid, ethyl hexadecanoate, ethyl linoleoate, kaurenol, an isomer of kaurenoic acid, spathulenol, hexadecanoic acid, 9,12,15-octadecatrienoic acid, cupressenic acid, isopropiloxigrandifloric acid, 2-5-ciclohexadiene-1,4-dione,2,6-bis, 1-octadecene, octadecanoic acid, ester diterpenic, caryophyllene oxide, 10, 13-octadecadienoic acid, isobutiloxigrandifloric acid, trans-cariofileno, 8,11-octadecadienoic acid, lupeol, lupeol www.intechopen.com Toxicity and Drug Testing 300 acetate, benzoylgrandifloric and cinnamoylgrandifloric acids (Oliveira et al, 1993;Moura et al, 2002;Biavatti et al, 2004;Santos, 2005;Yatsuda et al, 2005;Bertolucci et al, 2008;Alves et al, 2009;Bolina et al, 2009;Muceneeki et al, 2009). In quantitative terms, the most prevalent metabolites of hydroalcoholic extracts are coumarin (1,2-benzopyrone) (Biavatti et al, 2004;Bueno & Bastos, 2009), o-coumaric acid (Santos, 2005), dihydrocoumarin (Alves et al, 2009), syringaldehyde (Muceneeki et al, 2009) and kaurenoic acid (Vilegas et al, 1997a;Vilegas et al, 1997b;Yatsuda et al, 2005;Bertolucci et al, 2008). These substances have been associated with the therapeutic effects of guaco because they have anti-inflammatory and bronchodilator properties.…”