A validated dispersive liquid-liquid microextraction method for extraction of ochratoxin A from raisin samples

Karami-Osboo, Rouhollah; Miri, Ramin; Javidnia, Katayoun; Kobarfard, Farzad; AliAbadi, Mohammad Hossein Shojaee; Maham, Mehdi

doi:10.1007/s13197-013-1215-4

Cited by 21 publications

(11 citation statements)

References 20 publications

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“…As regards the retail unpacked dried vine fruits, 1 kg was purchased, from which 5 incremental samples weighing 100 g each were taken; consequently, the laboratory samples were 500 g that were homogenized in a Waring blender. Analytical method was similar to that described by Karami‐Osboo and others () with slight modifications.…”

Section: Methodsmentioning

confidence: 99%

Raisins and Currants as Conventional Nutraceuticals in Italian Market: Natural Occurrence of Ochratoxin A

Fanelli

Cozzi

Raiola

et al. 2017

Journal of Food Science

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The healthy consumers make a strong pressure to natural products that can prevent the chronic diseases and improve the general health status, and therefore an important aspect that have to be considered is the safe level of the nutraceuticals. This study reports the occurrence of Ochratoxin A (OTA) and associated fungal contamination in 35 samples of dried vine fruits imported in the European community potentially used for the development of new nutraceutical supplements. High pressure liquid chromatography analysis identified 18 samples as contaminated by OTA with an average level of 2.6 μg/kg. OTA was measured in 4 samples of currants (mean value of 6.6 μg/kg) and 13 samples of raisins (mean value of 1.4 μg/kg). In one sample of currants and one of raisins from Turkey OTA exceeded the limits set by European Commission of 10 μg/kg, being contaminated with 12.61 and 15.99 μg/kg, respectively. All the positive samples were confirmed by Orbitrap Q Exactive through their molecular weight and the corresponding fragmentation. The worldwide consumption of dried vine fruits contributed to OTA exposure in several group of consumers. In particular, considering the potential nutraceutical approach, this consumption may be represent a severe risk for healthy consumers that consider these products like healthy and salutistic for their contents in antioxidants, flavonoids, and polyphenols. Data reported in this study confirmed the need to regularly monitor mycotoxin levels in these food products and optimize the process of fruits drying in order to reduce the development of toxigenic molds.

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Section: Methodsmentioning

confidence: 99%

Raisins and Currants as Conventional Nutraceuticals in Italian Market: Natural Occurrence of Ochratoxin A

Fanelli

Cozzi

Raiola

et al. 2017

Journal of Food Science

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“…The comparison of the developed microextraction method with other microextraction methods for the extraction and quantification of OTA is summarized in Table . As it is shown, the developed method has lower LOD value than the other methods.…”

Section: Resultsmentioning

confidence: 99%

“…DLPME has been used in the extraction of various organic and inorganic analytes from real samples . In DLPME, a mixture of extraction solvent (e.g.…”

Section: Introductionmentioning

confidence: 99%

Syringe‐to‐syringe dispersive liquid‐phase microextraction solidified floating organic drop combined with high‐performance liquid chromatography for the separation and quantification of ochratoxin A in food samples

Asadi¹

2017

J of Separation Science

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A new, simple, and rapid syringe-to-syringe dispersive liquid-phase microextraction with solidified floating organic drop was used for the separation and preconcentration of ochratoxin A from grain and juice samples before its quantification using high-performance liquid chromatography and fluorescence detection. Factors influencing the microextraction efficiency of ochratoxin A, such as sample solution pH, type and volume of organic extractant, salt concentration, number of injections, and volume of the sample, were studied and optimized. Under the optimum properties, the calibration graph showed linearity in the range of 65.0-700.0 ng/L (coefficient of determination = 0.9991). The limit of detection was 20.0 ng/L. The inter-day and intra-day relative standard deviations were in the range of 5.0-8.5%. This method was successfully applied for the quantification of ochratoxin A in grain and juice samples.

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“…A miniaturised liquid-liquid extraction procedure with some modifications was employed [26,27]. The first clean-up method involved the addition of Carrez I (0.451 mol L −1 ) and Carrez II (1.339 mol L −1 ) solutions (500 µL) to the fruit drink sample (40 mL), followed by centrifugation.…”

Section: Preparation Of Fruit Drink Samplesmentioning

confidence: 99%

Simultaneous Quantitative Assessment of Ochratoxin A, Patulin, 5-Hydroxymethylfurfural, and Bisphenol A in Fruit Drinks Using HPLC with Diode Array-Fluorimetric Detection

Hassan

Othman

et al. 2020

Foods

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The analysis of regulated contaminants in fruit drinks often requires suitable validated and rapid analytical methods for cost-effective food control, and is of considerable interest among the fruit beverage industry. This study demonstrated a rapid and sensitive high-performance liquid chromatography approach for the simultaneous determination of ochratoxin A (OTA), patulin (PAT), 5-hydroxymethylfurfural (HMF), and bisphenol A (BPA) in various fruit drinks. The separations were achieved using a C18 core-shell column with both photo-diode array and fluorimetric detections connected in series. A gradient system consisting of methanol and 0.1% formic acid at a flow rate of 1.2 mL min−1, thermostated at 35 °C, provided fast elution with run time <9 min. Sample pretreatment was optimised to enable extraction of all analytes from fruit drink matrices. The optimised method was validated. Correlation coefficients of R > 0.99 were achieved with detection limits of 0.5 ng mL−1 (OTA), 1.1 ng mL−1 (PAT), 7.9 ng mL−1 (HMF), and 1.0 ng mL−1 (BPA). Recoveries ranged from 82% to 99%. Good relative standard deviations for intraday retention times (≤3.54%) and peak area (≤3.5%) were achieved. The developed multi-contaminants analysis method was successfully applied to determine OTA, PAT, HMF, and BPA in various fruit drinks.

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A validated dispersive liquid-liquid microextraction method for extraction of ochratoxin A from raisin samples

Cited by 21 publications

References 20 publications

Raisins and Currants as Conventional Nutraceuticals in Italian Market: Natural Occurrence of Ochratoxin A

Raisins and Currants as Conventional Nutraceuticals in Italian Market: Natural Occurrence of Ochratoxin A

Syringe‐to‐syringe dispersive liquid‐phase microextraction solidified floating organic drop combined with high‐performance liquid chromatography for the separation and quantification of ochratoxin A in food samples

Simultaneous Quantitative Assessment of Ochratoxin A, Patulin, 5-Hydroxymethylfurfural, and Bisphenol A in Fruit Drinks Using HPLC with Diode Array-Fluorimetric Detection

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