A validated high performance liquid chromatography method for the analysis of thymol and carvacrol in Thymus vulgaris L. volatile oil

Hajimehdipoor, Homa; Shekarchi, M.; Khanavi, Mahnaz; Adib, Noushin; Amri, Mohamad Shahreen

doi:10.4103/0973-1296.66927

Cited by 63 publications

(18 citation statements)

References 6 publications

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“…[3] Researchers have proved that these plants are source of compounds with antioxidant,[4] anti-inflammatory,[5] anti-allergic,[6] anti-depression,[7] anti-hyperglycemic[8] and antimicrobial[9–11] properties. These activities are mostly related to their phenolic compounds content especially rosmarinic acid (RA), an ester of caffeic acid and 3,4-dihydroxyphenyllactic acid [Figure 1][1] which was isolated for the first time from Rosmarinus officinalis L. leaves and later found in other species of Labiatae and Boraginaceae.…”

Section: Introductionmentioning

confidence: 99%

Comparative study of rosmarinic acid content in some plants of Labiatae family

Shekarchi¹,

Hajimehdipoor²,

Saeidnia³

et al. 2012

Phcog Mag

195

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Background:Plants of Labiatae are used in traditional medicine and phytotherapy. Rosmarinic acid (RA) is a phenolic compound which is found in many genus of Labiatae and exhibits important biological activities.Materials and Methods:In this investigation, RA contents of 29 species of Labiatae named Salvia officinalis, Salvia limbata, Salvia virgata, Salvia hypoleuca, Salvia macrosiphon, Salvia choloroleuca, Melissa officinalis, Origanum vulgare, Lavandula angustifolia, Rosmarinus officinalis, Thymus daenensis, Thymus citriodorous, Thymus pubescens, Thymus vulgaris, Zataria multiflora, Mentha piperita, Mentha pulegium, Mentha longifolia, Mentha spicata, Mentha aquatica, Mentha crispa, Perovskia artemisoides, Zhumeria majdae, Satureja hortensis, Satureja khuzistanica, Satureja bachtiarica, Satureja atropatana, Satureja mutica and Satureja macrantha were determined by using high-performance liquid chromatographic method.Results:The results showed that RA content in different species of Labiatae was 0.0-58.5 mg g-1 of dried plants. The highest amount of RA was found in Mentha species especially M. spicata.Conclusion:M. spicata can be considered as a new source of rosmarinic acid .

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Section: Introductionmentioning

confidence: 99%

Comparative study of rosmarinic acid content in some plants of Labiatae family

Shekarchi¹,

Hajimehdipoor²,

Saeidnia³

et al. 2012

Phcog Mag

195

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“…monograph (USP-38) was verificated for assay of aloin in AF tablets [19]. The method verification was performed through selectivity, linearity, precision, recovery, LOD and LOQ [24–26]. …”

Section: Methodsmentioning

confidence: 99%

Formulation of an aloe-based product according to Iranian traditional medicine and development of its analysis method

et al. 2017

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BackgroundCurrently, people are more interested to traditional medicine. The traditional formulations should be converted to modern drug delivery systems to be more acceptable for the patients. In the present investigation, a poly herbal medicine “Ayarij-e-Faiqra” (AF) based on Iranian traditional medicine (ITM) has been formulated and its quality control parameters have been developed.MethodsThe main ingredients of AF including barks of Cinnamomum zeylanicum Blume and Cinnamomum cassia J. Presl, the rhizomes of Nardostachys jatamansi DC., the fruits of Piper cubeba L.f., the flowers of Rosa damascena Herrm., the oleo gum resin of Pistacia terebinthus L. and Aloe spp. dried juice were powdered and used for preparing seven tablet formulations of the herbal mixture. Flowability of the different formulated powders was examined and the best formulations were selected (F6&F7). The tablets were prepared from the selected formulations compared according to the physical characteristics and finally, F7 was selected and coated. Physicochemical characters of core and coated AF tablets were determined and the HPLC method for quantitation of aloin as a marker of tablets was selected and verified according to selectivity, linearity, precision, recovery, LOD and LOQ.ResultsThe results showed that core and coated AF tablets were in agreement with USP requirements for herbal drugs. They had acceptable appearance, disintegration time, friability, hardness, dissolution behavior, weight variation and content uniformity. The amount of aloin in tablets was found 123.1 mg/tab. The HPLC method for aloin determination in AF tablets was verified according to selectivity, linearity (5–500 μg/ml, r2:0.9999), precision (RSD: 1.62%), recovery (108.0%), LOD & LOQ (0.0053 & 0.0161 μg/ml).ConclusionsThe formulated tablets could be a good substitute for powder and capsules of AF in ITM clinics with a feasible and precise method for its quality control.Graphical abstractAyarij-e-Faiqra formulation

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“…The determination coefficient ( r 2 ) was calculated by means of the least-square analysis [18,19]. The calibration lines were achieved through two replicates of each concentration of hyperoside, rutin, isorhamnetin and kaempferol (1–10, 10–60, 10–70 and 0.3-1.6 μg/mL), to identify the extent of the total variability of the response that could be explained by the linear regression model.…”

Section: Methodsmentioning

confidence: 99%

“…It ensures that no loss or uptake occurred during the process [18,19]. The determination of this parameter was performed during the method by studying the recovery after a standard addition procedure, with two additional levels.…”

Section: Methodsmentioning

confidence: 99%

Development of a validated HPLC method for the simultaneous determination of flavonoids in Cuscuta chinensis Lam. by ultra-violet detection

et al. 2012

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BackgroundCuscuta species known as dodder, have been used in traditional medicine of eastern and southern Asian countries as liver and kidney tonic. Flavonoids are considered as the main biologically active constituents in Cuscuta plants especially in C. chinensis Lam.ObjectiveIn the present study, a fast, simple and reliable method for the simultaneous determination and quantization of C. chinensis flavonols including hyperoside, rutin, isorhamnetin and kaempferol has been developed.Materials and methodsThe chromatographic separation was carried out on a reversed phase ACE 5 C18 with eluting at a flow rate of 1 ml/min using a gradient with O-phosphoric acid 0.25% : acetonitrile for 42 min. UV spectra were collected across the range of 200–900 nm, extracting 360 nm for the chromatograms. The method was validated according to linearity, selectivity, precision, recovery, LOD and LOQ.ResultsThe method was selective for determination of rutin, hyperoside, isorhamnetin and kampferol. The calibration graphs of flavonols were linear with r2 > 0.999. RSDs% of intra- and inter-day precisions were found 1.3&3.4 for rutin, 1.5&2.8 for hyperoside, 1.3&3.3 for isorhamnetin and 1.7 & 2.9 for kaempferol which were satisfactory. LODs and LOQs were calculated as 1.73 & 8.19 for rutin, 0.09 & 4.19 for hyperoside, 2.09 & 6.3 for isorhamnetin and 0.18 & 0.56 for kaempferol. The recovery averages of above-mentioned flavonols were 90.3%, 97.4%, 98.7% and 90.0%, respectively.ConclusionThe simplicity of the method makes it highly valuable for quality control of C. chinensis according to quantization of flavonols.

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A validated high performance liquid chromatography method for the analysis of thymol and carvacrol in Thymus vulgaris L. volatile oil

Cited by 63 publications

References 6 publications

Comparative study of rosmarinic acid content in some plants of Labiatae family

Comparative study of rosmarinic acid content in some plants of Labiatae family

Formulation of an aloe-based product according to Iranian traditional medicine and development of its analysis method

Development of a validated HPLC method for the simultaneous determination of flavonoids in Cuscuta chinensis Lam. by ultra-violet detection

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