A validated HPLC analytical method for the analysis of solasonine and solamargine in in vitro skin penetration studies

Tiossi, Renata Fabiane Jorge; Costa, Juliana C. Da; Miranda, Mariza Abreu; Praça, Fabíola Silva Garcia; Bentley, Maria Vitória Lopes Badra; Bastos, Jairo Kenupp; McChesney, James D.

doi:10.1590/s0100-40422012001100038

Cited by 5 publications

(6 citation statements)

References 12 publications

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“…Validation was undertaken following the parameters described by the Brazilian Sanitary Vigilance Agency, 38 Food and Drug Administration (FDA), 39 Ribani and collaborators 40 and Tossi and collaborators, 41,42 including linearity, limits of detection (LOD) and quantitation (LOQ), selectivity, precision (intra-assay and inter-assay), accuracy and recov-ery. Thus, the analytical method was validated to quantify boldine samples obtained from plant extracts, and commercialized tablet, capsule and dragee formulations.…”

Section: Validation Of Hplc Methodsmentioning

confidence: 99%

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Study of Quality Assurance For Peumus Boldus M Products By Botanic Profiling, Extraction Optimization, HPLC Quantification And Antioxidant Assay

Teixeira¹,

Cabral²,

Sousa³

et al. 2016

Phcogj

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Introduction:The boldo leaf has several traditional folk medicinal uses, such as for gallbladder, hepatic problems, digestive disorders, rheuma tism and others. In the work reported herein, botanic profiling, extraction optimization by Soxhlet, quantification of boldine by an easy/simple to run HPLC method and antioxidant assay are proposed for the quality assurance of boldo dried leaves, standardized extracts, dried extracts, tablets and capsules. Materials and Methods: In this present work we are studying a systematic approach in the quality assurance study of quality assurance for Peumus boldus M products by the ascertainment of pharmacobotanic parameters for boldo identification, investigation of the extraction para meters by Soxhlet method, development and validation of an easy/simple to run HPLC method to quantify boldine in the raw drug, extracts, commercial tablets, capsules and coated tablets, and antioxidant assay. Results: The plant material was submitted to a pharmacognostic evaluation through morphoanatomical diagnosis, showing that starlike trichomes can be used for boldo authentication. The HPLC validated analytical method is reliable, accurate and precise for boldine quantification. Furthermore, the Soxhlet extraction conditions were optimized. Conclusion: The methods proposed in this paper can be used for the quality assurance of boldo dried leaves,

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Section: Validation Of Hplc Methodsmentioning

confidence: 99%

“…The results were expressed as mean recovery percentage (R) according to Equation (Eq. (4)): [40][41][42] …”

Section: Accuracy and Recovery Of Boldine From Plant Extractmentioning

confidence: 99%

Study of Quality Assurance For Peumus Boldus M Products By Botanic Profiling, Extraction Optimization, HPLC Quantification And Antioxidant Assay

Teixeira¹,

Cabral²,

Sousa³

et al. 2016

Phcogj

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“…Regardless of the technique, an adequate analytical method to assay the drug, which should be specific, precise, and accurate, must be used [21]. When choosing a method, it is important to consider that a small amount of drug is expected in the different skin layers.…”

Section: Introductionmentioning

confidence: 99%

Liquid chromatography method to assay tretinoin in skin layers: validation and application in skin penetration/retention studies

Oliveira

Andrade

Oliveira

et al. 2020

Heliyon

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A B S T R A C TA liquid chromatography (LC) method for the quantification of tretinoin (TTN) in different matrices (adhesive tape, cotton and porcine skin layers, stratum corneum, viable epidermis, and dermis) was validated and applied in in vitro porcine skin penetration/retention studies. This study proposes, for the first time, a method for assaying TTN in separated porcine skin layers (stratum corneum, viable epidermis, and dermis). The skin studies were carried out using tape stripping and cutaneous retention techniques. The procedures for the extraction of TTN from dermatological formulations (creams and gels) and biological and non-biological matrices used with the tape stripping and retention techniques were also evaluated. The LC method consisted of a mobile phase composed of a mixture of methanol, water, and glacial acetic acid (85:15:1, v/v); a C18 column used as the stationary phase; a flow rate of 1.0 mL min À1 ; an injection volume of 100 μL; and TTN detection at 342 nm. The method was linear in the range of 0.05-15.00 μg mL À1 (r ¼ 0.9999), and it was precise and accurate. The limit of detection (LOD) and limit of quantification (LOQ) were 0.0165 μg mL À1 and 0.0495 μg mL À1 , respectively. TTN was extracted from different matrices, showing good precision [relative standard deviation (RSD) of <5%] and accuracy (89.4-113.9%). This method was successfully applied in the evaluation of TTN skin retention/permeation from dermatological formulations (cream and gel). A higher penetration of TTN through the skin was achieved with the gel rather than the cream, showing the influence of the dosage form. Therefore, the developed method can easily be applied in porcine skin penetration/retention studies of dermatological formulations containing TTN, and it is able to discriminate the behaviours of the different formulations.

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“…18 It is also necessary to develop and validate an analytical method for this test, i.e., to predict whether an adequate amount of the drug is released from the formulation. 19 Numerous methods of analysis have been developed for profiling green tea constituents. Reversed-phase high performance liquid chromatography (RP-HPLC) followed by UV detection is considered the gold standard.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, to develop a phytotherapic compound, it is important to validate an analytical method with accuracy, selectivity, precision, and linearity over the specific range in which an analyte is analyzed. 19 In this context, the present work aims to: (i) develop and validate an RP-HPLC method for simultaneous quantification of EGCG, CAF, and GA in transdermal formulation of green tea and (ii) determine the in vitro flux rate of EGCG across a synthetic membrane.…”

Section: Introductionmentioning

confidence: 99%

GREEN TEA IN TRANSDERMAL FORMULATION: HPLC METHOD FOR QUALITY CONTROL ANDIN VITRODRUG RELEASE ASSAYS

Alves¹,

Almeida²,

Polonini³

et al. 2014

Química Nova

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RP-HPLC based analytical method for use in both quality control of green tea in a semisolid formulation and for in vitro drug release assays was developed and validated. The method was precise (CV < 5%), accurate (recovery between 98% and 102%), linear (R 2 > 0.99), robust, and specific for the determination of epigallocatechin 3-gallate (EGCG), caffeine (CAF), and gallic acid (GA). In a diffusion cell chamber, the release rate of EGCG was 8896.01 μg cm −2 . This data showed that EGCG will be able to exert its systemic activity when delivered though the transdermal formulation, due to its good flux rates with the synthetic membrane.

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A validated HPLC analytical method for the analysis of solasonine and solamargine in in vitro skin penetration studies

Cited by 5 publications

References 12 publications

Study of Quality Assurance For Peumus Boldus M Products By Botanic Profiling, Extraction Optimization, HPLC Quantification And Antioxidant Assay

Study of Quality Assurance For Peumus Boldus M Products By Botanic Profiling, Extraction Optimization, HPLC Quantification And Antioxidant Assay

Liquid chromatography method to assay tretinoin in skin layers: validation and application in skin penetration/retention studies

GREEN TEA IN TRANSDERMAL FORMULATION: HPLC METHOD FOR QUALITY CONTROL ANDIN VITRODRUG RELEASE ASSAYS

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