A validated liquid chromatography–tandem mass spectrometry method for the determination of l‐hyoscyamine in human plasma: Application in a clinical study

Abstract

Atropine is a racemic mixture of D-and L-hyoscyamine, but only L-hyoscyamine is the effective ingredient. In this study, a new, sensitive, stable, and selective LC/MS assay was developed for the determination of L-hyoscyamine and applied to a clinical study.The parent-product (m/z) transition pair of L-hyoscyamine was 290.1 ! 124.1. Chromatographic separations were performed using a chiral MZ column (250 mm  4.6 mm, 5.0 μm) by a stepwise gradient elution mode with n-hexane, isopropanol, and diethylamine as mobile phases. L-Hyoscyamine in human plasma was extracted by liquidliquid extraction. This assay displayed a good linearity over a concentration range of 20.0-400 pg/mL for L-hyoscyamine. The accuracy of the validation assay for L-hyoscyamine ranged from À2.7% to 4.5%, and the precision was within 6.3% coefficient of variation. L-Hyoscyamine in human plasma remained stable at different storage conditions. The method has been successfully applied to plasma samples obtained from a safety study in humans.

A Review of Patents and Innovative Biopolymer-Based Hydrogels

“…Patent CN106556654B, belonging to the agricultural product quality and safety testing field, relates to a method for kappa-carrageenan detecting in livestock meat, especially t application of a liquid chromatography-tandem mass spectrometry method [172].…”