A Validated RP-HPLC Method for Simultaneous Determination of Cefixime and Clavulanic Acid Powder in Pediatric Oral Suspension

Nepal, Utsav; Panthi, Vijay Kumar; Chaudhary, Narendra Kumar; Chaudhary, Samip

doi:10.1155/2022/8331762

Cited by 6 publications

(3 citation statements)

References 17 publications

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“…Linearity deals with the potentiality of analytical method to receive test findings that are correspondently dependent on the concentration over a certain range [ 33 ]. The average area received from HPLC analysis was organized in accordance with different concentrations of LCZ prior to achieving the calibration curve.…”

Section: Resultsmentioning

confidence: 99%

Formulation and Development of a Water-in-Oil Emulsion-Based Luliconazole Cream: In Vitro Characterization and Analytical Method Validation by RP-HPLC

Panthi

Nepal

2022

International Journal of Analytical Chemistry

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Luliconazole (LCZ) is a new antifungal agent containing imidazole moiety which revealed broad-spectrum antifungal activity. The aim of this research was to prepare water-in-oil (w/o) emulsion-based cream formulation of LCZ in addition to the development and validation of an analytical method by reverse-phase high-performance liquid chromatography (RP-HPLC). Cetostearyl alcohol (12.14%), light liquid paraffin (5.00%), white soft paraffin (2.75%), and Tween-80 (1.00%) appeared as the optimized concentration to give better consistency to the cream. Moreover, without adding pH adjusting agents the pH of the optimized formulation (F5) was obtained within the range of human skin pH throughout the stability period. The value of particle size, polydispersity index, and zeta potential was 187.90 ± 2.061 nm, 0.124 ± 0.026, and -10.553 ± 1.349 mV, respectively. In this study, an analytical C18 (4.6 mm × 25 cm), 5 μm column was used for chromatographic separation with a mixture of acetonitrile and water in the proportion of 50 : 50 v/v as the mobile phase at a flow rate of 1.0 mL/min. The calibration curve was obtained linear at 296 nm in the concentration range of 0.08–0.12 mg/mL. Furthermore, the limit of detection (LOD) and limit of quantification (LOQ) were 0.0013 and 0.0042 µg/mL, respectively. In addition, the observed results demonstrated that our developed method was linear (R2 = 0.999), precise (%RSD below than 2.0%), and accurate (mean recovery% = 100.18–100.91). The F5 showed no physical changes until 6th month analysis at room temperature and accelerated conditions. Similarly, the assay obtained 101.99% ± 0.27 and 99.89% ± 0.08 at room temperature and accelerated conditions, respectively. Additionally, all validated parameters were obtained within the acceptable limit as well. These findings conclude that both physically and chemically stable w/o cream formulation of LCZ can be formulated and assessed for their stability by applying the authenticated analytical procedure of RP-HPLC.

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Section: Resultsmentioning

confidence: 99%

Formulation and Development of a Water-in-Oil Emulsion-Based Luliconazole Cream: In Vitro Characterization and Analytical Method Validation by RP-HPLC

Panthi

Nepal

2022

International Journal of Analytical Chemistry

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“…Individual and average percent recovery had to be between 98.0% and 102.0%, and the % RSD had to be below 2.0% at each level. 28…”

Section: Methods Validationmentioning

confidence: 99%

Development and validation of the RP-HPLC method for quantification of tavaborole

Prajapati,

Jain,

Bajpai

2024

Anal. Methods

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“…The stability of the sample is executed by keeping the samples for 0, 24, and 48 hr at room temperature 37 o C ± 2 o C and evaluated for the changes (Table 1). 25…”

Section: Stability Of Analytical Solutionmentioning

confidence: 99%

Synthesis, Characterization, RP-HPLC Method Development and Validation for Qualitative Estimation of (4Z) 3 Methyl 1 (4 Nitrobenzoyl) 1H Pyrazole 4,5 Dione 4[(4fluorophenyl) Hydrazone]

Ashtekar

Aggarwal

Raviraj

et al. 2023

Int J. Pharm. Investigation

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Aim: A new simple, accurate, precise, Reverse Phase High-performance Liquid Chromatography method was developed and validated for the estimation of newly synthesized (4Z) 3 methyl 1 (4 nitrobenzoyl) 1H pyrazole 4,5 dione 4[(4fluorophenyl)hydrazone] derivative of pyrazolone. Materials and Methods: The chromatogram was run through Luna 5µ C 18 (2). 250 × 4.80 mm. 272817-7 HPLC column; mobile phase consisting of acetonitrile and water in the ratio 90:10 was pumped through the column at a flow rate of 0.8mL/min. Results: The optimized wavelength was 237 nm. The retention time of (4Z) 3 methyl 1 (4 nitrobenzoyl) 1H pyrazole 4,5 dione 4[( 4fluorophenyl)hydrazone] was found to be 7.3 min. The percentage relative standard deviation was obtained as 0.3%. Conclusion: This method was accurate, precise, and sensitive; therefore, could be used for the quality control and bioanalytical evaluation of (4Z) 3 methyl 1 (4 nitrobenzoyl) 1H pyrazole 4,5 dione 4[(4fluorophenyl)hydrazone] in drug testing laboratory.

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A Validated RP-HPLC Method for Simultaneous Determination of Cefixime and Clavulanic Acid Powder in Pediatric Oral Suspension

Cited by 6 publications

References 17 publications

Formulation and Development of a Water-in-Oil Emulsion-Based Luliconazole Cream: In Vitro Characterization and Analytical Method Validation by RP-HPLC

Formulation and Development of a Water-in-Oil Emulsion-Based Luliconazole Cream: In Vitro Characterization and Analytical Method Validation by RP-HPLC

Development and validation of the RP-HPLC method for quantification of tavaborole

Synthesis, Characterization, RP-HPLC Method Development and Validation for Qualitative Estimation of (4Z) 3 Methyl 1 (4 Nitrobenzoyl) 1H Pyrazole 4,5 Dione 4[(4fluorophenyl) Hydrazone]

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