A versatile cell detector for cell volume measurements

Wb, Taylor

doi:10.1007/bf02477245

Cited by 12 publications

(4 citation statements)

References 5 publications

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“…Cell counts and cell volume determinations of the malignant T-lymphoblast cell lines MOLT-3 and Jurkat treated or untreated with TPA were determined by an electronic cell counter fitted with a pulse height analyzer as described previously by Taylor (1970). In short, MOLT-3 or Jurkat cells were cultured in a-medium supplemented with 10% fetal calf serum with or without 16 nM TPA.…”

Section: Cell Volume Determinationmentioning

confidence: 99%

Induction of human malignant T‐lymphoblastic cell lines MOLT‐3 and jurkat by 12‐O‐tetradecanoylphorbol‐13‐acetate: Biochemical, physical, and morphological characterization

Nagasawa

Howatson

Mak

1981

Journal Cellular Physiology

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The process of induction of human malignant T-lymphoblastic cell line MOLT-3 by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) was examined. It was found that the induction process by TPA, which included increase in cells with receptors to sheep red blood cells (E--rosette positive--E+) and decrease in the levels of the marker enzyme terminal deoxynucleotidyl transferase (TdT) was not affected by the presence of DNA synthesis inhibitor arabinofuranosylcytosine (Ara-C). The exposure time to TPA required to elicit these changes was found to be short, in the order of 1 hour or less. The kinetics of the increased in E+ cells, decrease in the levels of TdT in these cells, or decrease in the ability to proliferate as measured by colony formation were similar with exposure to TPA for 1, 6, 24, or 96 hours. We have examined the effect of antitumor promoter compounds on their ability to block induction of MOLT-3 cells by TPA. Results indicated that none of these compounds, dexamethasone, antipain, retinoic acid, and L-1-tosylamide-2-phenylethylchloromethyl ketone (TPCK), was effective in reducing the number of E+ cells induced by TPA. Examination of three other leukemic T-cell lines indicated that, in addition to MOLT-3, the leukemic T-cell line Jurkat also responded to TPA, whereas two other leukemic T-cells lines CCRF-CEM and CCRF-HSB-2 did not. Certain physical and morphological changes were also observed after stimulation of MOLT-3 cells and Jurkat cells by TPA. We found that, following the addition of TPA, the cell volumes of MOLT-3 cells decreased from an average of 1150 micrometers3 to about 500 micrometers3, whereas those of Jurkat were reduced to about 700 micrometers3 from 1100 micrometers3. Electron microscopic studies of these lymphoblasts also revealed that after treatment with TPA the induced cells were generally smaller in size with increase in the density of the nuclear materials and condensation of the chromatin structures.

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Section: Cell Volume Determinationmentioning

confidence: 99%

Induction of human malignant T‐lymphoblastic cell lines MOLT‐3 and jurkat by 12‐O‐tetradecanoylphorbol‐13‐acetate: Biochemical, physical, and morphological characterization

Nagasawa

Howatson

Mak

1981

Journal Cellular Physiology

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“…By the late 1960s, studies related to origins of volumetric artifacts were burgeoning, 28,30,44,45,49,65–78 and several groups were building experimental systems based on the Coulter Principle. 28–30,41,45–47,73,79 The troublesome sizing artifact arose when particles passed through the sensing aperture at different radial distances from its axis. 65 At low effective particle concentrations, apertures having lengths several times their diameter were shown to improve sizing resolution, 34,41,67,80 as did an auxiliary flow surrounding a smaller suspension stream so as to hydrodynamically focus particles through the aperture near its axis.…”

Section: Act IIImentioning

confidence: 99%

“…65 At low effective particle concentrations, apertures having lengths several times their diameter were shown to improve sizing resolution, 34,41,67,80 as did an auxiliary flow surrounding a smaller suspension stream so as to hydrodynamically focus particles through the aperture near its axis. [81][82][83][84][85] At typical particle concentrations, sampling signal pulses at aperture midpoint 67 or selecting them according to duration 79 substantially improved volumetric accuracy (see overviews 65,67,78,[86][87][88].…”

Section: Act IIImentioning

confidence: 99%

The Coulter Principle: Foundation of an Industry

Graham

2003

JALA: Journal of the Association for Laboratory Automation

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Introduced in the mid-1950s, the Coulter Principle became the foundation of an industry responding to the need for automated cell-counting instruments. The industry developed in three acts, as Wallace H. Coulter and his brother Joseph R. Coulter, Jr., developed the simple idea of passing cells through a sensing aperture. In Act I, Wallace's desire to automate the routine erythrocyte count led to a simple idea, the definition of the Coulter Principle, its patenting, its acceptance by the National Institutes of Health, and its description at a national conference. In Act II, the Coulter brothers addressed the practicalities of a commercial instrument and of a business organization to support its manufacture and sale. In Act III, a broad research effort developed regarding volumetric errors originating in functional characteristics of the sensing aperture, and the brothers' growing organization found solutions permitting introduction of increasingly automated hematology analyzers. Today the industry thrives, with several participants.

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“…Discrimination between the red and white cells, which measure approximately 8 and 20ju,m, respectively, is obtained from the different sizes of electrical signals. 10 3.4 3.4.1…”

Section: Techniquesmentioning

confidence: 99%

Biomedical engineering

Perkins¹

1971

Proc. Inst. Electr. Eng. UK

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The increasing sophistication of modern technology and its widening areas of application have established the need for multidisciplinary activities, and biomedical engineering is a typical example of this trend. The evolution of the subject from the engineering approach of earlier biomedical workers is described, and some of the lessons learned before and during the transition are considered. An approach to this now rather extensive subject is made by separating the application to biomedical research from that to clinical medicine, The research section is stratified according to the level of biological organisation with which it is concerned, that is, from the molecular systems of biochemistry to the organ systems of physiology. At each level, sufficient background is given to show the relevance of biomedical engineering, and this approach is continued with the clinical applications, which are considered within conventional medical categories. The principal contribution to medicine is found to lie with the innovation of the new methods made possible by advancing technology. The more recent technological ideas of organ and function replacement are discussed followed by that of computer usage in various areas of biomedicine. In conclusion, a critical assessment of present achievements is attempted, and the possible future of biomedical engineering is discussed.

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A versatile cell detector for cell volume measurements

Cited by 12 publications

References 5 publications

Induction of human malignant T‐lymphoblastic cell lines MOLT‐3 and jurkat by 12‐O‐tetradecanoylphorbol‐13‐acetate: Biochemical, physical, and morphological characterization

Induction of human malignant T‐lymphoblastic cell lines MOLT‐3 and jurkat by 12‐O‐tetradecanoylphorbol‐13‐acetate: Biochemical, physical, and morphological characterization

The Coulter Principle: Foundation of an Industry

Biomedical engineering

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