A Versatile Fiber‐Optic Fluorescence Sensor Based on Molecularly Imprinted Microstructures Polymerized in Situ

Abstract

Seeing molecules: A method using laser‐induced photopolymerization was developed to generate highly selective fiber optic sensors in a few seconds that are based on molecularly imprinted polymer (MIP) microtips. The fluorescence detection signal was enhanced using gold nanoparticles. The sensor also detects nonfluorescent analytes when a fluorescent signaling monomer is incorporated into the MIP.

Molecularly Imprinted Polymer Coated Quantum Dots for Multiplexed Cell Targeting and Imaging

“…At the same time,w ev erified that there was no self-initiated polymerization, ap henomenon frequently observed in the presence of numerous monomers and under lower-wavelength UV light. [17,18] MIPs are tailormade synthetic antibody mimics that can recognize and bind target molecules specifically.They are synthesized by copolymerizing functional and cross-linking monomers in the presence of am olecular template,t hus resulting in the formation of binding sites with affinities and specificities comparable to those of natural antibodies.T heir molecularrecognition properties,c ombined with ah igh chemical and physical stability,m ake them interesting substitutes for antibodies in immunoassays, [19] biosensors, [20] bioseparation, [18,21] controlled drug release, [22] and bioimaging. [12][13][14][15] Consequences include effects on tumor growth, escape from apoptosis,m etastasis formation, and resistance to therapy.P olysaccharides involved in the glycosylation procedure have ah ighly conserved simple composition and are ubiquitously expressed in all animals that have ad eveloped immune response.T he natural production of antibodies that specifically recognize these "weak antigens" is difficult; [16] hence,traditional immunohistochemical methods for detecting glycosylations on cells are rare.A na lternative would be "plastic antibodies" or MIPs.…”

Fiber Optic Sensors Based on Nanostructured Materials

“…Following this idea, optical sensors have been developed to detect anthracene [92] and aluminum ions in aqueous media [93]. This idea has been applied to develop optical fiber sensors able to detect chemical solvents [94] and cocaine [95].…”

Efficient one-pot synthesis of hydrophilic and fluorescent molecularly imprinted polymer nanoparticles for direct drug quantification in real biological samples

“…Among them, fluorescent MIPs-based chemosensors are particularly interesting because fluorescence has proven to be a highly powerful transduction mechanism to report the chemical recognition event (due to the easy availability of many fluorescence techniques, their high sensitivity, broad linear range, little sampling, and high simplicity of operation) (Basabe-Desmonts et al, 2007;Canfarotta et al, 2013;Henry et al, 2005;Takeuchi et al, 2005). So far, many fluorescent MIPs-based chemosensors have been designed by incorporating fluorescent components (e.g., organic fluorescent moieties or inorganic quantum dots) into MIPs for the sensitive and label-free detection of a wide range of analytes (Awino and Zhao, 2014;Banerjee and König, 2013;Chao et al, 2014;Huy et al, 2014;Ivanova-Mitseva et al, 2012;Li et al, 2010;Tan et al, 2014;Ton et al, 2013;Turkewitsch et al, 1998;Wan et al, 2013;Wei et al, 2014;Wu et al, 2015;Yang et al, 2012;Zhang et al, 2014;Zhao et al, 2012). One of the main focuses in this field is to develop fluorescent MIPs that are able to directly detect small organic molecules in aqueous media because food, environmental, and clinical analyses are typically based on aqueous samples.…”