A versatile method for analysis of saliva, plasma and urine for total thiols using HPLC with UV detection

Stachniuk, Justyna; Kubalczyk, Paweł; Furmaniak, Paulina; Głowacki, Rafał

doi:10.1016/j.talanta.2016.04.031

Cited by 63 publications

(34 citation statements)

References 28 publications

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“…It is well-understood that the minimum concentration of an analyte that can be detected is known as the LOD, while the lowest amount of an analyte in a sample that can be quantitatively determined with suitable precision is the LOQ. In a previous study, the LOD and LOQ was determined as “the concentration that produces a detector signal that could be easily known from the baseline (three times larger than the baseline noise)” and the LOQ as “the concentration that gives a detector signal 10 times larger than the baseline noise” (Stachniuk et al, 2016 ). In our study, we followed the above guidelines, and the signal-to-noise ratio (S/N) determined for detection limit (LOD) is equal to 3:1 and for the LOQ was 10:1.…”

Section: Resultsmentioning

confidence: 99%

Development of a UPLC-FLD Method for Detection of Aflatoxin B1 and M1 in Animal Tissue to Study the Effect of Curcumin on Mycotoxin Clearance Rates

Cui

Ishfaq

Li³

et al. 2017

Front. Pharmacol.

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Aflatoxin B1 (AFB1) and its metabolite aflatoxin M1 (AFM1) are well-known carcinogens for humans and animals health. In this study, an ultra-high performance liquid chromatography linked with fluorescence detection (UPLC-FLD) method was optimized and validated. In addition, we investigated for the first time, the influence of curcumin on residue depletion of AFB1 and AFM1 in liver, kidney, and muscle tissues of broiler chickens and estimated a necessary clearance time required for AFB1 and AFM1 residues. The results showed that the average recoveries of AFB1 varied in liver, kidney, and muscles between 82.32–85.56, 85.34–88.45, and 84.88–89.73% respectively, while the average recoveries of AFM1 in liver, kidney, and muscles varied between 92.17–95.03, 94.12–97.21, and 95.32–98.51%, respectively. The detection limit of aflatoxin B1 was 0.008 ng/ml, while for aflatoxin M1 was 0.003 ng/ml. The limit of quantification (LOQ) for AFB1 and AFM1 was 0.02 and 0.01 ng/ml, respectively. Clearance time for AFB1 and AFM1 residues were analyzed in two experimental groups of broilers. One group fed with dietary AFB1 (5.0 mg/kg feed) and other with curcumin+AFB1 diet (curcumin; 300 mg/kg feed, AFB1; 5.0 mg/kg feed). AFB1 and AFM1 residues clearance time was calculated based on LOQ using withdrawal time calculation software (WT1.4). Clearance time analyzed for AFB1 ranged from 11 to 19 days and for AFM1 ranged from 10 to 12 days at 95% confidence level. Interestingly, curcumin supplementation in the diet reduced the clearance time of AFM1 in liver and kidney but not in muscle tissues. Conclusively, the developed method can be appropriately used for the quality control testing of commercial broiler-meat processing companies, food manufacturers, and quality control laboratories.

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Section: Resultsmentioning

confidence: 99%

Development of a UPLC-FLD Method for Detection of Aflatoxin B1 and M1 in Animal Tissue to Study the Effect of Curcumin on Mycotoxin Clearance Rates

Cui

Ishfaq

Li³

et al. 2017

Front. Pharmacol.

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“…These S-linked forms are converted to the reduced form and analyzed as the total Hcy. The disulfide can be reduced using sodium borohydride [25,26], tributylphosphine (TBP) [27,28], tris(2-carboxyethyl)phosphine (TCEP) [6,[29][30][31][32][33][34][35][36][37][38][39][40], dithiothreitol (DTT) [41][42][43][44], 1,4-dithioerythritol (DTE) [45][46][47], and mercaptoethanol [48]. Bai et al developed a unique online reduction quartz column packed with a Zn(II)-TCEP complex [33,49].…”

Section: Hplc Analysis Of Homocysteine and Related Compoundsmentioning

confidence: 99%

“…As mentioned above, this is addressed by derivatization using reagents such as those shown in Figure 2. The halopyridine-type derivatization reagents 2-chloro-1-methylquinolium tetrafluoroborate (CMQT) [27,39,40], 2-chloro-1-methyllepidinium tetrafluoroborate (CMLT) [6,30], and 1-benzyl-2-chloropyridinium bromide (BCPB) [26] react with Hcy and related compounds to form stable S-quinolinium or S-pyridinium derivatives with intense UV absorption. Stachniuk et al developed an HPLC-UV/Vis method using CMLT derivatization for the determination of Hcy, Cys, GSH, GluCys, CysGly, and NAC in human saliva, plasma, and urine [30].…”

Section: Uv/vis Detectionmentioning

confidence: 99%

HPLC Analysis of Homocysteine and Related Compounds

Wada¹,

Nakamura²,

Nakashima³

2018

Non-Proteinogenic Amino Acids

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Homocysteine (Hcy), a sulfur-containing amino acid, is a representative intermediate metabolite of methionine (Met) to cysteine (Cys) via several intermediates. An elevated level of Hcy in plasma plays an important role in diseases such as neural tube defects and Down syndrome. Homocystinuria is the most common inborn error of sulfur metabolism and is caused by mutations in the metabolic enzymes of Hcy. These errors can be caused by abnormal levels of Met metabolites and classified on the basis of plasma Met levels. Additionally, Hcy and related compounds such as glutathione play an important role in maintaining homeostasis. Therefore, the simultaneous determination of Hcy and/or related compounds is required for appropriate clinical management of several diseases. The sulfur-containing amino acids and their derivatives in biological samples are quantified sensitively using high-performance liquid chromatography methods coupled with various detection methods such as UV/Vis, fluorescence, chemiluminescence, electrochemical, mass spectrometry, and tandem mass spectrometry. In this chapter, we review recent advances in these analytical methods and their applications.

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“…Mercapto compounds, including cysteine, homocysteine, and glutathione, play an essential role in the daily activity of lives. The alteration of the level of the mercapto compounds in bio-system may forecast some critical diseases, such as cancer or cardiovascular disease [ 34 , 35 , 36 , 37 , 38 ]. Here, a kind of mercapto compound, p -mercapto benzoic acid ( p -MBA), was involved in the test.…”

Section: Introductionmentioning

confidence: 99%

Label-Free and Reproducible Chemical Sensor Using the Vertical-Fluid-Array Induced Optical Fiber Long Period Grating (VIOLIN)

Long

et al. 2020

Sensors

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Fiber optical refractometers have gained a substantial reputation in biological and chemical sensing domain regarding their label-free and remote-operation working mode. However, the practical breakthrough of the fiber optical bio/chemosensor is impeded by a lack of reconfigurability as well as the explicitness of the determination between bulk and surface refractive indices. In this letter, we further implement the highly flexible and reproducible long period grating called “VIOLIN” in chemical sensing area for the demonstration of moving those obstacles. In this configuration, the liquid is not only leveraged as the chemical carrier but also the periodic modulation of the optical fiber to facilitate the resonant signal. The thiol compound that is adsorbed by the fluidic substrate can be transduced to the pure alteration of the bulk refractive index of the liquid, which can be sensitively perceived by the resonant drift. Taking advantage of its freely dismantled feature, the VIOLIN sensor enables flexible reproduction and high throughput detection, yielding a new vision to the fiber optic biochemical sensing field.

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A versatile method for analysis of saliva, plasma and urine for total thiols using HPLC with UV detection

Cited by 63 publications

References 28 publications

Development of a UPLC-FLD Method for Detection of Aflatoxin B1 and M1 in Animal Tissue to Study the Effect of Curcumin on Mycotoxin Clearance Rates

Development of a UPLC-FLD Method for Detection of Aflatoxin B1 and M1 in Animal Tissue to Study the Effect of Curcumin on Mycotoxin Clearance Rates

HPLC Analysis of Homocysteine and Related Compounds

Label-Free and Reproducible Chemical Sensor Using the Vertical-Fluid-Array Induced Optical Fiber Long Period Grating (VIOLIN)

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