A novel naphthalimide molecule, 4-(2-methoxyethoxy)-N-butyl-1,8-naphthalimide (MEBN), has been synthesized and characterized. The UV-visible absorption and fluorescence emission spectral properties in n-hexane, toluene, CHCl 3 , and CH 3 OH were studied. Its optical properties depend on solvent polarity. The application of MEBN in cell imaging was investigated.