A water soluble extract of radish greens ameliorates high fat diet-induced obesity in mice and inhibits adipogenesis in preadipocytes

Oh, Min Seok; Lee, Hyun Hee L.; Lee, Hye-Bin; Ho, Moon; Park, Miri; Lee, Chang‐Hyun; Park, Ho-Young

doi:10.1039/d1fo04152e

Cited by 6 publications

(2 citation statements)

References 48 publications

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“…6-, 8-, 10-gingerol and 6-, 8-and 10-shogaol, could decrease the lipid content in both 3T3-L1 cells undergoing adipogenic differentiation and in mature adipocytes derived from this cell line. Several studies evaluated the ability of plant-derived extracts to reduce the lipid content in the 3T3-L1 cell line (8,(23)(24)(25)(26). These phenols were evaluated individually in different experimental models that support their antitumor (27,28), anti-inflammatory (29,30) and anti-obesogenic capacities (31).…”

Section: Discussionmentioning

confidence: 99%

A mix of ginger phenols exhibits anti‑adipogenic and lipolytic effects in mature adipocytes derived from 3T3‑L1 cells

Gembe‑Olivarez,

Preciado‑Ortiz,

Campos‑Perez

et al. 2023

Exp Ther Med

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The prevalence of obesity has increased rapidly worldwide. Obesity is characterized by excessive adipose tissue in the body, which is related to hyperplasia and hypertrophy in adipocytes. Ginger ( Zingiber officinale Roscoe ) is a medicinal plant that possesses an anti-obesogenic effect mostly attributed to gingerols, the most abundant bioactive compounds in ginger. The anti-adipogenic and lipolytic effects of these phenols have been shown when investigated individually. Therefore, the present study aimed to evaluate the lipolytic and anti-adipogenic activity of a mix of the main ginger phenols 6-gingerol, 8-gingerol, 10-gingerol, 6-shogaol, 8-shogaol and 10-shogaol on the 3T3-L1 cell line. A total of four study groups were designed: Negative control (3T3-L1 preadipocytes); positive control (mature 3T3-L1 adipocytes); phenols-pre (3T3-L1 cells stimulated with the phenols mix during adipogenic differentiation); and phenols-post (mature 3T3-L1 adipocytes stimulated with the phenols mix). MTT viability cell assay and Oil Red O staining were performed. Glycerol concentration supernatants were determined using the VITROS 350 Chemistry System. Expression of mRNA was measured using qPCR. The treatment with a 2 µg/ml ginger phenol dose reduced the lipid content by 45.52±7.8 and 35.95±0.76% in the phenols-pre and -post group, respectively, compared with that in the positive control group. The phenols-post group presented a higher glycerol concentration in the supernatant compared with that in the positive control and the phenols-pre groups. The mRNA expression levels of CCAAT/enhancer-binding protein alpha, peroxisome proliferator activated receptor- γ , fatty acid-binding protein 4 and fatty acid synthase were higher in the phenols-pre and lower in the phenols-post groups, compared with those in the positive control group. To the best of our knowledge, the current study demonstrated for the first time the anti-adipogenic and lipolytic effects of a mix of the main bioactive compounds found in ginger, and it also established the basis to use this mix of phenols in in vivo studies and clinical trials.

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Section: Discussionmentioning

confidence: 99%

A mix of ginger phenols exhibits anti‑adipogenic and lipolytic effects in mature adipocytes derived from 3T3‑L1 cells

Gembe‑Olivarez,

Preciado‑Ortiz,

Campos‑Perez

et al. 2023

Exp Ther Med

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“…However, their limited harvest season and lack of commercialization have hindered them from being traditional specialty foods. A previous study showed that a water-soluble radish green extract reduces body fat and improves the gut environment in mice [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Prebiotic and Anti-Adipogenic Effects of Radish Green Polysaccharide

Lee

Kim

et al. 2023

Microorganisms

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Radish (Raphanus sativus L.) greens are consumed as a source of nutrition, and their polysaccharides such as rhamnogalacturonan-I possess certain beneficial properties. This study investigated the prebiotic effects of a radish green polysaccharide (RGP) on gut health and obesity. The prebiotic activity of RGP was evaluated based on the pH changes and short-chain fatty acids (SCFAs) concentration. The results showed that 0.5% RGP had a higher prebiotic activity score than inulin and increased SCFAs production in all five prebiotic strains. Moreover, RGP inhibited fat accumulation in 3T3-L1 adipocytes, indicating its potential to reduce obesity. Overall, these findings suggested that the polysaccharide of radish greens has prebiotic effects and may serve as a beneficial prebiotic for gut health and obesity.

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Micro-grinding–based production for sulforaphene-enriched radish seeds extract via facilitating glucosinolates-myrosinase reaction, and evaluation of its anti-adipogenic effects

et al. 2023

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A water soluble extract of radish greens ameliorates high fat diet-induced obesity in mice and inhibits adipogenesis in preadipocytes

Abstract: Radish (Raphanus sativus L.) is used for dietary source and its greens have reported functionalities. This study aimed to investigate the effects of a water-soluble extract from radish greens (WERG)...

Cited by 6 publications

References 48 publications

A mix of ginger phenols exhibits anti‑adipogenic and lipolytic effects in mature adipocytes derived from 3T3‑L1 cells

A mix of ginger phenols exhibits anti‑adipogenic and lipolytic effects in mature adipocytes derived from 3T3‑L1 cells

Prebiotic and Anti-Adipogenic Effects of Radish Green Polysaccharide

Micro-grinding–based production for sulforaphene-enriched radish seeds extract via facilitating glucosinolates-myrosinase reaction, and evaluation of its anti-adipogenic effects

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