No abstract

Oxidative stress, caused by the excessive production of reactive oxygen species (ROS), results in cellular damage. Therefore, functional materials with antioxidant properties are necessary to maintain redox balance. Turmeric leaves (Curcuma longa L. leaves; TL) are known to have antioxidant properties, including 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2′-Azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS), and Hydrogen peroxide (H2O2) radical scavenging activity in several studies. The antioxidant effects of TL come from distinct bioactive compounds, such as curcumin, total phenolic compounds, and flavonoids. Therefore, in this study, the antioxidant effects of a water extract of TL (TLE) against H2O2 treatment were assessed in vitro Vero cells and in vivo zebrafish models. The intracellular ROS generation and the proportion of sub-G1 phase cells were evaluated in H2O2- or/and TLE-treated Vero cells to measure the antioxidant activity of TLE. TLE showed outstanding intracellular ROS scavenging activity and significantly decreased the proportion of cells in the sub-G1 phase in a dose-dependent manner. Furthermore, cell death, ROS generation, and lipid peroxidation in the H2O2-treated zebrafish model were attenuated as a consequence of TLE treatment. Collectively, the results from this study suggested that TLE may be an alternative material to relieve ROS generation through its antioxidant properties or a suitable material for the application in a functional food industry.

No abstract

Radish (Raphanus sativus L.) is used for dietary source and its greens have reported functionalities. This study aimed to investigate the effects of a water-soluble extract from radish greens (WERG)...

Radish (Raphanus sativus) has been reported to exert antioxidant, anti-microbial, anti-coagulant, anti-hyperlipidemic, and anti-hypertensive effects. However, there have been no reports so far on the comparison of active ingredients and adipogenesis inhibitory activity between various parts of the radish. In this study, we analyzed the glucoraphenin and ferulic acid content in radish root and leaf extracts using a UPLC TQ-MS system. Also, their adipogenesis inhibitory effects were examined using 3T3-L1 preadipocytes. The results of this study showed that radish leaf extract has higher glucoraphenin (11,971.8 μg/g in EtOH extract) and ferulic acid (851.7 μg/g in EtOH extract) content than radish root extracts and the adipocyte differentiation suppressing activity was also higher than the radish root extracts. These results indicate that the radish leaf is rich in active ingredients and has a higher inhibitory effect on adipocyte differentiation than the radish root. Therefore, radish leaves could be used in the active compound extraction and development of functional material for adipogenesis suppression.