Comparison of Active Ingredient Contents and Adipocyte Differentiation Suppressing Effect in Radish Roots and Leaves

Abstract

Radish (Raphanus sativus) has been reported to exert antioxidant, anti-microbial, anti-coagulant, anti-hyperlipidemic, and anti-hypertensive effects. However, there have been no reports so far on the comparison of active ingredients and adipogenesis inhibitory activity between various parts of the radish. In this study, we analyzed the glucoraphenin and ferulic acid content in radish root and leaf extracts using a UPLC TQ-MS system. Also, their adipogenesis inhibitory effects were examined using 3T3-L1 preadipocytes. The results of this study showed that radish leaf extract has higher glucoraphenin (11,971.8 μg/g in EtOH extract) and ferulic acid (851.7 μg/g in EtOH extract) content than radish root extracts and the adipocyte differentiation suppressing activity was also higher than the radish root extracts. These results indicate that the radish leaf is rich in active ingredients and has a higher inhibitory effect on adipocyte differentiation than the radish root. Therefore, radish leaves could be used in the active compound extraction and development of functional material for adipogenesis suppression.