Resistance exercise is recommended to individuals following high-protein diets in order to augment changes in body composition. However, alterations in macronutrient composition may compromise physical performance. The present study investigated the effects of an isoenergetic high-protein diet on upper and lower limb strength and fatigue during high-intensity resistance exercise. Ten recreationally active women, aged 25-40 years, followed a control diet (55, 15 and 30 % of energy from carbohydrate, protein and fat, respectively) and a high-protein diet (respective values, 30, 40 and 30) for 7 d each in a random counterbalanced design. Each participant underwent strength testing of upper limb (isometric handgrip strength and endurance) and lower limb (four sets of sixteen maximal knee flexions and extensions on an isokinetic dynamometer) before and after applying each diet. Body weight, body fat and RER were significantly reduced following the high-protein diet (P, 0·05). No differences were found between diets in any of the strength performance parameters (handgrip strength, handgrip endurance, peak torque, total work and fatigue) or the responses of heart rate, systolic and diastolic arterial pressure, blood lactate and blood glucose to exercise. Women on a short-term isoenergetic high-protein, moderate-fat diet maintained muscular strength and endurance of upper and lower limbs during high-intensity resistance exercise without experiencing fatigue earlier compared with a control diet.High-protein diet: Fatigue: Resistance exercise: Handgrip: Women A considerable percentage of the population has reported using a low-carbohydrate, high-protein diet for weight loss and/or maintenance (1) . Although low-carbohydrate ketogenic diets have produced favourable effects on body weight, they have raised concerns on health issues, since these diets are accompanied by increased fat and protein intake. Another criticism of these dietary plans was that they did not recommend physical activity as an integral part of weight loss (1) .Recently proposed high-protein diets advocate the consumption of lean protein sources and allow unrefined carbohydrates from fruits and vegetables. Such high-protein (35 -45 % of energy), low-carbohydrate (20 -35 %) and moderate-fat (, 30 %) diets (high-protein diets) have attracted much attention and are frequently recommended for weight loss and maintenance to individuals with obesity or diabetes (2,3) . There is evidence that high-protein diets induce a number of favourable changes along with weight loss (reduction in body fat, improvement in lipidaemic and glycaemic profiles and resting blood pressure) (2,4) . The addition of exercise (aerobic and/or resistance) to a high-protein diet had additive effects on body composition during weight loss (5) .Despite the popularity of high-protein diets, few studies have investigated their effects on exercise performance (6,7) . These studies reported that a 7 d high-protein diet decreased endurance (aerobic) performance of recreational and trained athle...