Studies of the elements that make up the structure of a building have generally focused on topics related to their physical and structural capacities. Although research has been carried out into environmental impact during the life cycle stages, the environmental profile is far from established. This research aims to reduce the gap in the knowledge of this subject, offering useful information to professionals in the construction industry, which will enable them to consider environmental aspects when choosing the best construction systems. The present study applies the methodology of the life cycle assessment (LCA), to analyze and compare four floor construction systems in two different scenarios (“A” with a functional homogeneous unit of 1 m2 and “B” with 1 m² made up of the percentages of the floor system and the special areas of the building). The analysis is performed using the LCA Manager software, along with the Ecoinvent 3.1 database and with a cradle to handover perspective (A1–A5). Comparison was made using two environmental impact methodologies, Eco-indicator 99 and CML 2001. The results highlight the stages A1–A3 as those that generate the greatest environmental impact. Comparing the environmental profiles of the different floor systems, one-way floor systems I and II had the best environmental scores, 30% less than two-way floor system III and 50% less than slab floor system IV.