A wideband picosecond pulsed electric fields (psPEF) exposure system for the nanoporation of biological cells

Gao, Mingxiang; Wang, Siqi; Shang, Sen; Zhao, Jiping; Lu, Xiaoyun

doi:10.1016/j.bioelechem.2021.107790

Cited by 8 publications

(4 citation statements)

References 36 publications

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“…Various types of devices are used in the treatment of cells and tissues with the pulsed electric fields, such as pulsed focusing antennas and phased antenna arrays [Guo et al, 2014a], microwave horn antennas, point electrode systems [Schoenbach et al, 2008], flat and wire electrode systems [Kenaan et al, 2011], strip lines [Soueid et al, 2018], microstrip lines [Merla et al, 2018; Gao et al, 2021], and coaxial waveguides [Soueid et al, 2018]. There are systems with capacitive and resistive coupling between the high‐voltage electrodes and the cell culture medium.…”

Section: Introductionmentioning

confidence: 99%

The Action of the Pulsed Electric Field of the Subnanosecond Range on Human Tumor Cells

Petrov

Moraleva

Антипова

et al. 2022

Bioelectromagnetics

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The action of the pulsed electric field of the subnanosecond range on Jurkat, HEK 293, and U-87 MG human cell lines was studied. The cells were treated in a waveguide in 0.18 ml electrodeless Teflon cuvettes. The electric field strength in the cell culture medium was ~2 kV/cm, the pulse duration was ~1 ns, the leading edge was 150 ps, the frequency was 100 Hz, and the treatment time was 5 min. According to estimates, the change of the transmembrane potential during the pulse was ~20 mV and we assume that it was insufficient for electroporation. Jurkat and HEK 293 cells appeared to be more resistant to the treatment than U-87 MG cells. We have observed that the impulses with the above-mentioned parameters can cause a noticeable change in the mitochondrial activity of U-87 MG cells. Bioelectromagnetics. 43:327-335, 2022.

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Section: Introductionmentioning

confidence: 99%

The Action of the Pulsed Electric Field of the Subnanosecond Range on Human Tumor Cells

Petrov

Moraleva

Антипова

et al. 2022

Bioelectromagnetics

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“…The number of papers reporting the effects of electric and/or electromagnetic fields with picosecond rise-times on biological systems is quite low. Considering the cellular level, Gao et al [27] reported that an extremely high intensity pulsed electric field with 150 ps rise-time is able to permeabilize biological cells. In the works considering the influence of pulsed electromagnetic fields at the protein level, the studied range of pulse rise-times is limited to nanoseconds [28].…”

Section: Introductionmentioning

confidence: 99%

The Impact of Fast-Rise-Time Electromagnetic Field and Pressure on the Aggregation of Peroxidase upon Its Adsorption onto Mica

et al. 2021

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Our present study concerns the influence of the picosecond rise-time-pulsed electromagnetic field, and the impact of nanosecond pulsed pressure on the aggregation state of horseradish peroxidase (HRP) as a model enzyme. The influence of a 640 kV/m pulsed electromagnetic field with a pulse rise-time of ~200 ps on the activity and aggregation state of an enzyme is studied by the single-molecule atomic force microscopy (AFM) method. The influence of such a field is shown to lead to aggregation of the protein and to a decrease in its enzymatic activity. Moreover, the effect of a shock wave with a pressure front rise-time of 80 ns on the increase in the HRP aggregation is demonstrated. The results obtained herein can be of use in modeling the impact of electromagnetic and pressure pulses on enzymes and on whole living organisms. Our results are also important for taking into account the effect of pulsed fields on the body in the development of drugs, therapeutic procedures, and novel highly sensitive medical diagnosticums.

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“…Significant changes in the membrane conductance have been observed in RHN, NG108 and CHO cells treated with bursts of twenty-five pulses of 190 kV/cm and 500 ps duration at 1 kHz [3]. More recently, 260 ps duration pulses of about 10 kV/cm, applied for 3 seconds at a pulse repetition frequency (PRF) of 1 kHz have been shown to generate permeabilization to calcium ions in U937 lymphoma cells [4].…”

mentioning

confidence: 99%

Cells Electroporation With Subnanosecond Pulsed Electric Fields

Vallet,

Ibrahimi,

Ariztia

et al. 2023

Proceedings of the XXXVth URSI General Assembly and Scientific Symposium – GASS 2023

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A wideband picosecond pulsed electric fields (psPEF) exposure system for the nanoporation of biological cells

Cited by 8 publications

References 36 publications

The Action of the Pulsed Electric Field of the Subnanosecond Range on Human Tumor Cells

The Action of the Pulsed Electric Field of the Subnanosecond Range on Human Tumor Cells

The Impact of Fast-Rise-Time Electromagnetic Field and Pressure on the Aggregation of Peroxidase upon Its Adsorption onto Mica

Cells Electroporation With Subnanosecond Pulsed Electric Fields

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